Degradation of the Naphthalene

 

K. Thangavel

HOD, Department of Chemistry, AMET University, Chennai

 

ABSTRACT:

Naphthalene is one of the easy Polycyclic Aromatic Hydrocarbon (PAH). In the field of oil refineries and fossil fuels, the Polycyclic Aromatic Hydrocarbon is one of the main constraints and the contents are toxic and carcinogenic. In this work the bacterial strain was isolated and the isolates are determined by the biochemical measurements. By using the isolations, the naphthalene degradations are achieved. In this paper, the naphthalene degradations are performed.

 

 

 

 

INTRODUCTION:

Nowadays, one of the most important environmental issues is hydrocarbon defilement. This is the following from the movements recognized with oil refineries, accidental arrivals of oil based commodities and a number of attribute speculate are of cautious watchful in the earth[1-8]. The effects of the Naphthalene for human are listed below.

·        Inhalation

·        Ingestion

·        Dermal contact

·        Anemia

·        Liver Damage

 

RESOURCES AND METHODS:

Composition of media:

Contents: Yeast extract, Nacl, Agar, Casein, Distilled water.

 

Soil samples:

By using the five petrol pumps, the soil samples are collected. The soli collected from the ground is saved in the sterile polythene bags.

 

Agar plating method:

In the test tubes, the various soil samples are mixed with the saline contents. The sterile petri is poured and solidified. Then the soil samples is mixed and saved in the 38 degree for 24 hrs.

 

Culturing:

The tubes and media were sterilized and the media was poured. The culture plate was taken and incubated at 39 degree for 32 hrs.

 

SIMULATION RESULTS:

Table 1: Characteristics of the Bacteria

Description	Strain Of Bacteria
Gram Discoloration	Constructive
Character	Shaft
Oxygen constraint	Aerobic
Catalase	Constructive
Indole manufacture	Constructive
Methyl red Examination	Constructive

 

 

Table 2: Normal Degradations

Time Durations	Sample 1	Sample 2	Sample 3	Sample 4	Sample 5
12 Hours	3.98±0.006	2.81±0.001	4.81±0.008	3.00±0.001	2.58±0.003
24 Hours	5.48±0.012	1.86±0.002	6.61±0.004	6.59±0.001	5.65±0.001
48 Hours	9.50±0.658	6.66±0.002	8.65±0.004	10.28±1.423	7.88±0.001

 

Figure 1: Graphical Analysis of the Degradations

 

 

CONCLUSIONS:

Bacillus sp. was out-of-the-way and illustrious from the five characteristic soil tests. The expertise of the infinitesimal organisms to humiliate naphthalene was examined and it establishes to humiliate PAH. In this current evaluation it is established that biodegradation of naphthalene from Bacillus subtilis gathered from soil test it was documented as gram as Bacillus sp. by gram recoloring and by biochemical test. We can suggest Bacillus subtilis as appropriate life forms utilized as a part of zones infected by naphthalene. Albeit, many strains have been previously disconnected for naphthalene biodegradation, the ebb and flow walk around employment is projected that this living wage being turned out to be higher to the previously separated strains for the biodegradation of naphthalene.

 

REFERENCES:

1.       Meckenstock, R.U., Annweiler, E., Michaelis, W., Richnow, H.H. and Schink, B., Anaerobic naphthalene degradation by a sulfate-reducing enrichment culture. Applied and Environmental Microbiology, 2000, 66(7), pp.2743-2747.

2.       Bosch, R., Garcı́a-Valdés, E. and Moore, E.R., Genetic characterization and evolutionary implications of a chromosomally encoded naphthalene-degradation upper pathway from Pseudomonas stutzeri AN10. Gene, 1999, 236(1), pp.149-157.

3.       Manohar, S., C. K. Kim, and T. B. Karegoudar. "Enhanced degradation of naphthalene by immobilization of Pseudomonas sp. strain NGK1 in polyurethane foam." Applied Microbiology and Biotechnology (2001), 55, no. 3: 311-316.

4.       Daane, L.L., Harjono, I., Barns, S.M., Launen, L.A., Palleron, N.J. and Häggblom, M.M., PAH-degradation by Paenibacillus spp. and description of Paenibacillusnaphthalenovorans sp. nov., a naphthalene-degrading bacterium from the rhizosphere of salt marsh plants. International journal of systematic and evolutionary microbiology, 2002, 52(1), pp.131-139.

5.       Jeon, C.O., Park, W., Ghiorse, W.C. and Madsen, E.L.,. Polaromonasnaphthalenivorans sp. nov., a naphthalene-degrading bacterium from naphthalene-contaminated sediment. International Journal of Systematic and Evolutionary Microbiology, 2004, 54(1), pp.93-97.

6.       Garcia-Valdes E, Cozar E, Rotger R, Lalucat J, Ursing J. New naphthalene-degrading marine Pseudomonas strains. Applied and environmental microbiology. 1988 Oct 1;54(10):2478-85.

7.       Tsuda, M. and Iino, T., Naphthalene degrading genes on plasmid NAH7 are on a defective transposon. Molecular and General Genetics MGG, 1990, 223(1), pp.33-39.

8.       Liu, Z., Jacobson, A.M. and Luthy, R.G., Biodegradation of naphthalene in aqueous nonionic surfactant systems. Applied and environmental microbiology, 1995, 61(1), pp.145-151.

 

 

 

 

Accepted on 26.08.2017        © RJPT All right reserved