Author(s):
Priyanka M. Pandya, Pankaj P. Kapupara, Ketan V. Shah
Email(s):
ketan.shah@rku.ac.in
DOI:
10.5958/0974-360X.2018.00165.8 
Address:
Priyanka M. Pandya, Pankaj P. Kapupara, Ketan V. Shah*
School of Pharmacy, R K University, Kasturbadham, Rajkot, Gujarat, India-360020
*Corresponding Author
Published In:
Volume - 11,
Issue - 3,
Year - 2018
ABSTRACT:
A simple, efficient and reproducible method for the simultaneous determination of Clotrimazole and its Impurity (2-Chlorphenyl diphenyl methanol) has been developed using reversed phase high performance liquid chromatography. The separation was carried out using a mobile phase consisting of methanol: acetonitrile (95:5 % v/v). Column used was Zorbax C18 (100 × 4.6 mm internal diameter) 3.5 µm with flow rate of 1 ml/min. The detection wavelength used was 225 nm. The retention time of Clotrimazole and 2-Chlorophenyl diphenyl methanol were 2.81 and 2.14 respectively. Linearity of Clotrimazole and its Impurity are in range of 10-80 µg/mL and 2-12 µg/mL respectively. Analytical parameters were calculated and a statistical evaluation included.
Cite this article:
Priyanka M. Pandya, Pankaj P. Kapupara, Ketan V. Shah. Development and Validation of an Analytical Method for Simultaneous Estimation of Clotrimazole and its Impurity by RP-HPLC. Research J. Pharm. and Tech. 2018; 11(3): 894-898. doi: 10.5958/0974-360X.2018.00165.8
Cite(Electronic):
Priyanka M. Pandya, Pankaj P. Kapupara, Ketan V. Shah. Development and Validation of an Analytical Method for Simultaneous Estimation of Clotrimazole and its Impurity by RP-HPLC. Research J. Pharm. and Tech. 2018; 11(3): 894-898. doi: 10.5958/0974-360X.2018.00165.8 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2018-11-3-13