ABSTRACT:
Objective: we have synthesized the silver nanoparticles (AgNPs) by using the soil fungus Beauveria bassiana, the results recorded from UV-vis spectrophotometer and scanning electron microscopy (SEM) support the biosynthesis and characterization of AgNPs. The synthesized silver nanoparticles have also been tested against the larvae and pupae of Culex pipiens. Methods: The synthesized silver nanoparticles tested against larvae and pupae of Culex pipiens, the efficacy test was achieved at different concentrations (150, 200 and 250 ppm) The results: second larval instar of Cx. pipiens were high susceptible to synthesized AgNPs while, pupae were found less susceptible to synthesized AgNPs as the highest mortality rate were 98.88%, 95.55%, and 94.44% for second, third and fourth larval in star respectively at concentration 250ppm after 24 h, and 50.00% for pupa at the same concentration after 48 h. Conclusion: The results described in this study open the possibility for further investigations of the efficacy of larvicidal and properties of natural products, the beneficial point of silver nanoparticles as larvicides is that the insecticides resistance, environmental pollution, toxic hazards to humans and other non-target organism due to the overuse of pesticides can be overcome