Author(s):
Survi Mishra, S. T. Narenderan, B. Babu, Kuntal Mukherjee, S. N. Meyyanathan
Email(s):
babu@jssuni.edu.in
DOI:
10.5958/0974-360X.2019.00938.7 
Address:
Survi Mishra, S. T. Narenderan, B. Babu*, Kuntal Mukherjee, S. N. Meyyanathan
Department of Pharmaceutical Analysis, JSS College of Pharmacy, Udhagamandalam, Tamil Nadu,
JSS Academy of Higher Education & Research, India.
*Corresponding Author
Published In:
Volume - 12,
Issue - 11,
Year - 2019
ABSTRACT:
A simple, sensitive and rapid high performance liquid chromatographic method has been developed for the determination of Gemcitabine. Chromatographic separation was achieved on anInertsil ODS-3V column (250mm x 4.6mm; 5µm) with a mobile phase consisting of water: acetonitrile (90:10; pH 3.5 adjusted with orthophosphoric acid) with a flow rate of 1.0ml/min (UV detection at 270nm). Linearity was observed over the concentration range of 1-120µg/ml with a regression equation y=19773x+ 86063 and having a regression value (R2)= 0.998. The limit of detection (LOD) and limit of quantification (LOQ) values found to be 10ng/ml and 20ng/ml, respectively. The percentage recovery of the marketed formulation was found to be 100.08%. Validation revealed that the preferred method was specific, accurate, precise, reliable, robust, reproducible and suitable for the quantitative analysis.
Cite this article:
Survi Mishra, S. T. Narenderan, B. Babu, Kuntal Mukherjee, S. N. Meyyanathan. Validated Analytical Method for the Estimation of Gemcitabine from its Pharmaceutical Formulation by RP-HPLC. Research J. Pharm. and Tech. 2019; 12(11): 5407-5412. doi: 10.5958/0974-360X.2019.00938.7
Cite(Electronic):
Survi Mishra, S. T. Narenderan, B. Babu, Kuntal Mukherjee, S. N. Meyyanathan. Validated Analytical Method for the Estimation of Gemcitabine from its Pharmaceutical Formulation by RP-HPLC. Research J. Pharm. and Tech. 2019; 12(11): 5407-5412. doi: 10.5958/0974-360X.2019.00938.7 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2019-12-11-50