Author(s):
Vaishali P. Patel, Krishna A. Bhatt, Mrunali M. Shah, Dharti M. Patil, Rakshita S. Patel, Divya M. Desai, Prachi H. Parmar
Email(s):
vp42468@gmail.com
DOI:
10.5958/0974-360X.2021.00012.3 
Address:
Vaishali P. Patel*, Krishna A. Bhatt, Mrunali M. Shah, Dharti M. Patil, Rakshita S. Patel, Divya M. Desai, Prachi H. Parmar
Department of Quality Assurance, Parul Institute of Pharmacy and Research, Parul University, Waghodia Road - 390019 Vadodara, Gujarat, India.
*Corresponding Author
Published In:
Volume - 14,
Issue - 1,
Year - 2021
ABSTRACT:
Scientifically estimation of Vildagliptin in bulk and pharmaceutical dosage forms by 3-methyl-2-bezothiazoline hydrazone reagent using longer wavelength give strong absorbance. The purpose of this analytical validation procedure is to validate using ICH guideline.3-methyl-2-bezothiazoline hydrazone reacts with ferric chloride in the presence of amine group, Resulting green coloured species scanned in uv-visible spectrophotometric with the range 400-800nm. Linearity concentration ranges10 to 50µg/ml and it given absorbance at 620nm, Correlation coefficient value 0.998, Recovery studies obtain within range of 98.41 to 99.75% at maximum absorbance 620nm. This method can be successfully applied for the estimation of drug content in pharmaceutical formulations. Resulting Analytical Validate method suitable for its intended use.
Cite this article:
Vaishali P. Patel, Krishna A. Bhatt, Mrunali M. Shah, Dharti M. Patil, Rakshita S. Patel, Divya M. Desai, Prachi H. Parmar. Development and Validation of Spectrophotometric Estimation of Vildagliptin through Oxidative Coupling Reaction using MBTH Reagent in Pharmaceutical Dosage Form. Research J. Pharm. and Tech. 2021; 14(1):62-66. doi: 10.5958/0974-360X.2021.00012.3
Cite(Electronic):
Vaishali P. Patel, Krishna A. Bhatt, Mrunali M. Shah, Dharti M. Patil, Rakshita S. Patel, Divya M. Desai, Prachi H. Parmar. Development and Validation of Spectrophotometric Estimation of Vildagliptin through Oxidative Coupling Reaction using MBTH Reagent in Pharmaceutical Dosage Form. Research J. Pharm. and Tech. 2021; 14(1):62-66. doi: 10.5958/0974-360X.2021.00012.3 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2021-14-1-12
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