Author(s): Kunjal Vegad, Nimisha Kakadia, Niranjan Kanaki

Email(s): , ,

DOI: 10.5958/0974-360X.2021.00016.0   

Address: Kunjal Vegad, Nimisha Kakadia, Niranjan Kanaki
Sharda School Pharmacy, Pethapur-Mahudi Road, Pethapur, Gandhinagar. K.B. Institute of Pharmaceutical Education and Research Centre, Gandhinagar, Gujarat, India.
*Corresponding Author

Published In:   Volume - 14,      Issue - 1,     Year - 2021

Osteoporosis is a silent disease leading to complications with the progression in the disease. Osteoporosis is characterized by a decrease in bone mass and microarchitectural deterioration of bone tissue, leading to enhanced bone fragility and a consequent increase in fracture risk. During the early menopausal stage in women, there is reduction in circulating estrogen which leads to an increase in the rate of bone resorption. Hence, there is an imbalance between bone formation and resorption which lead to osteoporosis. Many traditional plants and phytoconstituents have been used as potential therapy for the treatment of the osteoporosis. However, the scientific approach for the validation of the use of phytoconstituents or herbs in the treatment of osteoporosis is the need of an hour. In present work five ethnomedicinal plants, Asparagus racemosus, Hemidesmus indicus, Berberis aristata, Emblica officinalis, and Nigella sativa were selected on the basis of their use in bone diseases in literature. The selected plants were studied for the positive effect on cell proliferation which is one of the important targets for the bone remodeling. Cell proliferative activity of aqueous and ethanolic extracts of selected plants was screened using MTT assay. Osteosarcoma cells (MG-63 cell line) and primary mesenchymal stem cells (MSCs) isolated from the rat femur were used for the MTT assay. The results of the above study showed significant (P < 0.001)cell proliferative activity of aqueous extract of seeds of Nigella sativaon MG-63 cells and MSCs and hence, supporting its ethnomedicinal use in the management of osteoporosis.

Cite this article:
Kunjal Vegad, Nimisha Kakadia, Niranjan Kanaki. Cell Proliferative Potential of Ethnomedicinal plants for the management of Osteoporosis. Research J. Pharm. and Tech. 2021; 14(1):85-90. doi: 10.5958/0974-360X.2021.00016.0

Kunjal Vegad, Nimisha Kakadia, Niranjan Kanaki. Cell Proliferative Potential of Ethnomedicinal plants for the management of Osteoporosis. Research J. Pharm. and Tech. 2021; 14(1):85-90. doi: 10.5958/0974-360X.2021.00016.0   Available on:

1.    Das BG, et al. Prevalence and risk factors of osteopenia and osteoporosis in Indian women. vol. 2016; 15:15-18.
2.    Strukov V, et al. The use of Bone Tissue Non-Steroid Anabolizators in Treatment of Osteoporosis. Research Journal of Pharmacy and Technology. 2019;12(5):2195-99.
3.    Sakat BT, et al. Osteoporosis: The Brittle Bone. Asian Journal of Pharmaceutical Research. 2018;8(1):39-43.
4.    Jalil A, et al. Role of medicinal plants and natural products on osteoporotic fracture healing. Evidence-Based Complementary and Alternative Medicine. 2012;2012.
5.    Shirwaikar A, Khan S. Medicinal plants for the management of post menopausal osteoporosis: A review. The Open Bone Journal. 2010;2:1-13.
6.    Orsini LS, et al. Health care utilization and expenditures in the United States: a study of osteoporosis-related fractures. Osteoporosis International. 2005;16(4):359-71.
7.    Iqbal MM. Osteoporosis: epidemiology, diagnosis, and treatment. Southern Medical Journal. 2000;93(1):2-18.
8.    Kenny AM, Prestwood KM. Osteoporosis: pathogenesis, diagnosis, and treatment in older adults. Rheumatic Disease Clinics of North America. 2000; 26(3): 569-91.
9.    Anderson JJ, et al. Calcium intake from diet and supplements and the risk of coronary artery calcification and its progression among older adults: 10‐year follow‐up of the Multi‐Ethnic Study of Atherosclerosis (MESA). Journal of the American Heart Association. 2016;5(10): e003815.
10.    Wanjari AS, Wanjari DS. An Overview on Herbal Medicine. Research Journal of Pharmacognosy and Phytochemistry. 2019;11(1):14-17.
11.    Medicine IDoISo, Homoeopathy. The ayurvedic pharmacopoeia of India: Govt. of India, Ministry of Health and Family Welfare, Dept. of ISM & H.; 2007.
12.    Strober W. Trypan blue exclusion test of cell viability. Current Protocols in Immunology. 2015;111(1): A3. B. 1-A3. B. 3.
13.    Altman SA, et al. Comparison of trypan blue dye exclusion and fluorometric assays for mammalian cell viability determinations. Biotechnology Progress. 1993; 9(6): 671-74.
14.    Louis KS, Siegel AC. Cell viability analysis using trypan blue: manual and automated methods. Mammalian Cell Viability: Springer; 2011. pp. 7-12.
15.    Huang S, et al. An improved protocol for isolation and culture of mesenchymal stem cells from mouse bone marrow. Journal of Orthopaedic Translation. 2015;3(1):26-33.
16.    Nadri S, et al. An efficient method for isolation of murine bone marrow mesenchymal stem cells. International Journal of Developmental Biology. 2002;51(8):723-29.
17.    Potu BK, et al. Petroleum ether extract of Cissus quadrangularis (Linn.) enhances bone marrow mesenchymal stem cell proliferation and facilitates osteoblastogenesis. Clinics. 2009; 64(10): 993-98.
18.    Zhu H, et al. A protocol for isolation and culture of mesenchymal stem cells from mouse compact bone. Nature protocols. 2010; 5(3): 550.
19.    Ferrari M, et al. MTT colorimetric assay for testing macrophage cytotoxic activity in vitro. Journal of Immunological Methods. 1990;131(2):165-72.
20.    Jiao L, et al. Antiosteoporotic activity of phenolic compounds from Curculigo orchioides. Phytomedicine. 2009;16(9):874-81.
21.    Pavithra S, Banu N. A First Report on the Antiproliferative activity of Sodium Copper Chlorophyllin from Endangered Medicinal Plant Rhinacanthus nasutus on HepG2 and HeLa Cell Lines. Research Journal of Pharmacy and Technology. 2017; 10(1): 325-29.
22.    Curtis EM, et al. Reprint of: the impact of fragility fracture and approaches to osteoporosis risk assessment worldwide. International Journal of Orthopaedic and Trauma Nursing. 2017; 26: 7-17.
23.    Khoshnood Z, et al. A Description of Osteoporosis Preventive Behaviors in Iranian Adolescent Girls. Asian Journal of Nursing Education and Research. 2016;6(1):1-4.
24.    General R. Census Commissioner, India. Census of India. 2011;2000.
25.    Shah KK. Survey on Relation between Menopause and Oral Health. Research Journal of Pharmacy and Technology. 2015; 8(8): 1150-52.
26.    Suman V, et al. Effect of variable diet and physical activity on bone mineral density in adults using peripheral-Dexa scan. Research Journal of Pharmacy and Technology. 2018;11(6):2404-07.
27.    Padmanabhan K, et al. Which is more prevalent among the female population-Osteopenia or Osteoporosis? A cross sectional study. Research Journal of Pharmacy and Technology. 2019;12(3):1163-68.
28.    Helmberg A. Bone Metabolism. Dostopno na URL: http://www helmberg at/bone-metabolism pdf Pridobljeno. 2013;12.
29.    Radhakrishna B, et al. Current and Future Trends of Drugs Used in Osteoporosis. Research Journal of Pharmacology and Pharmacodynamics. 2011;3(6):329-33.
30.    Boivin G, Meunier P. Effects of bisphosphonates on matrix mineralization. Journal of Musculoskeletal and Neuronal Interactions. 2002;2(6):538-43.
31.    Czekanska EM, et al. A phenotypic comparison of osteoblast cell lines versus human primary osteoblasts for biomaterials testing. Journal of biomedical materials research Part A. 2014;102(8):2636-43.
32.    De Mattei M, et al. Correlation between pulsed electromagnetic fields exposure time and cell proliferation increase in human osteosarcoma cell lines and human normal osteoblast cells in vitro. Bioelectromagnetics: Journal of the Bioelectromagnetics Society, The Society for Physical Regulation in Biology and Medicine, The European Bioelectromagnetics Association. 1999;20(3):177-82.
33.    Manolagas SC. Birth and death of bone cells: basic regulatory mechanisms and implications for the pathogenesis and treatment of osteoporosis. Endocrine Reviews. 2000;21(2):115-37.
34.    Tiwari P, et al. Nigella sativa: Phytochemistry, Pharmacology and its Therapeutic Potential. Research Journal of Pharmacy and Technology. 2019;12(7):3111-16.

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