Author(s): Prerana Venkatachalam, Varalakshmi Kilingar Nadumane


DOI: 10.5958/0974-360X.2021.00027.5   

Address: Prerana Venkatachalam, Varalakshmi Kilingar Nadumane*
Department of Biotechnology, School of Sciences, JAIN (Deemed-to-be University), 18/3, 9th Main, 3rd block, Jayanagar, Bengaluru – 560011, India.
*Corresponding Author

Published In:   Volume - 14,      Issue - 1,     Year - 2021

As a pink coloured bioactive fraction, B5, produced by B. endophyticus JUPR15 was showing promising anti-cancer properties, response surface methodology (RSM) was employed to enhance its yield. Various carbon, nitrogen, amino acids, vitamins and metal ions along with varying pH, temperature and incubation time were screened independently for enhancing pigment and biomass yield. Central Composite Design (CCD) of RSM was applied to evaluate the optimal conditions for the chosen factors – glycerol, mannitol and malt extract, which were studied at five levels. The R2 value for biomass was 0.9874 and that for pigment yield was 0.9931. The observed responses were in close agreement with the predicted responses. Through CCD, a 14.31-fold (12.02 g/L) increase in biomass and 7.77-fold (202.2 mg/L) increase in pigment yields were achieved as opposed to the 0.84 g/L and 26.03 mg/L of biomass and pigment yields obtained through control conditions. The model was found to be significant (P<0.01) for both the responses. Besides this, the addition of mannitol alone gave 191.36 mg/L of pigment, closer to that obtained via optimized conditions. It can be concluded that both single factor and RSM can be employed to increase the anti-cancer pigment production.

Cite this article:
Prerana Venkatachalam, Varalakshmi Kilingar Nadumane. Enhanced Production of an Anti-Cancer Pigment from Bacillus endophyticus JUPR15: Single Factor System Vs RSM. Research J. Pharm. and Tech. 2021; 14(1):153-161. doi: 10.5958/0974-360X.2021.00027.5

Prerana Venkatachalam, Varalakshmi Kilingar Nadumane. Enhanced Production of an Anti-Cancer Pigment from Bacillus endophyticus JUPR15: Single Factor System Vs RSM. Research J. Pharm. and Tech. 2021; 14(1):153-161. doi: 10.5958/0974-360X.2021.00027.5   Available on:

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