G Nivedha Kumari, S.S.M Umamageswari
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G Nivedha Kumari1, S.S.M Umamageswari2
1Principal Investigator, MBBS Student, Saveetha Medical College, Saveetha Institute of Medical and Technical Science, Thandalam, Chennai 602105.
2Professor, Department of Microbiology, Saveetha Medical College, Saveetha Institute of Medical and Technical Science, Thandalam, Chennai 602105.
Volume - 14,
Issue - 1,
Year - 2021
CONS are one of the most frequent causes of nosocomial outbreaks in hospitals and are reservoirs of multiple antimicrobial resistant determinants. Identification and speciation of CONS has become important due to the growing recognition and medical importance to define their clinical significance. S.caprae has the ability to produce a biofilm in vitro which itself serves as a virulent factor for many infections. The study was to screen MRSA in nasal swabs among health care workers. Routinely isolation of genus staphylococci was done based on catalase test. For differentiating coagulase positive and coagulase negative staphylococci were done based on slide coagulase and tube coagulase test. CONS species was identified by Vitek 2 system Most important virulence factor in CONS spcies was biofilm production. So we checked biofilm production in cons species. Out of 82 nasal swabs that were collected from both right and left nostril, organisms were found in 72(87.8%) samples of both right and left nostril. Staphylococcus caprae was found predominantly in 56(77.7%) of 72 samples. 8(11.1%) samples showed the growth of Staphylococcus lugdunensis. Staphylococcus schleiferi and Staphylococcus cohnii were found in four sample each. Out of 56 S.caprae, 12(21.4%) samples showed strong biofilm production, 28(50%) showed moderate and 16(28.5%) showed weak. Our study shows a large number Staphylococcus caprae from the nasal swabs, that has got the significant property of biofilm production which itself serves as a virulent factor for causing infections. So, some of the CONS are considered pathogenic and it should be speciated to avoid deaths caused by infections
Cite this article:
G Nivedha Kumari, S.S.M Umamageswari. Speciation of Cons Isolated from Nasal Swabs among Health Care Workers from A Tertiary Care Centre with special Reference to S. caprae and its Virulence Factor – Biofilm Production. Research J. Pharm. and Tech. 2021; 14(1):385-388. doi: 10.5958/0974-360X.2021.00070.6
G Nivedha Kumari, S.S.M Umamageswari. Speciation of Cons Isolated from Nasal Swabs among Health Care Workers from A Tertiary Care Centre with special Reference to S. caprae and its Virulence Factor – Biofilm Production. Research J. Pharm. and Tech. 2021; 14(1):385-388. doi: 10.5958/0974-360X.2021.00070.6 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2021-14-1-70
1. AA L, Chincholkar VV, Nilekar SL, More SR. Identification of Coagulase Negative Staphylococci and Their Antibiogram Isolated from Various Clinical Specimen. JMRR. 2017:63.
2. Singh S, Banerjee G, Agarwal SK, Kumar M, Singh RK. Simple method for speciation of clinically significant coagulase negative Staphylococci and its antibiotic sensitivity/resistant pat-tern in NICU of tertiary care centre.
3. von Eiff C, Peters G, Heilmann C. Pathogenesis of infections due to coagulasenegative staphylococci. The Lancet infectious Diseases. 2002 Nov 1; 2(11):677-85.
4. Becker K, Heilmann C, Peters G. Coagulase-negative staphylococci. Clinical Microbiology reviews. 2014 Oct 1; 27(4): 870-926.
5. Seng P, Barbe M, Pinelli PO, Gouriet F, Drancourt M, Minebois A, Cellier N, Lechiche C, Asencio G, Lavigne JP, Sotto A. Staphylococcus caprae bone and joint infections: a re-emerging infection?. Clinical Microbiology and Infection. 2014 Dec 1; 20(12): O1052-8.
6. Kini GD, Parris AR, Tang JS. A rare presentation of sepsis from Staphylococcus caprae. The Open Microbiology Journal. 2009; 3:67.
7. Yaron S, Römling U. Biofilm formation by enteric pathogens and its role in plant colonization and persistence. Microbial Biotechnology. 2014 Nov;7(6):496-516.
8. Collee JG, Marr W. Specimen collection, culture containers and media. Mackie & McCartney Practical Medical Microbiology. Collee JG, Fraser AG, Marmion BP, Simmons A (ed): Churchill Livingstone, New York. 1996:85-111.
9. Umamagewari SS, Sree PN, Mukerjee M. Comparative Study between Conventional Methods over Automated Methods for Speciation of Coagulase Negative Staphylococcus in a Tertiary Care Center. Int. J. Curr. Microbiol. App. Sci. 2016; 5(10):639-47.
10. Mathur T, Singhal S, Khan S, Upadhyay DJ, Fatma T, Rattan A. Detection of biofilm formation among the clinical isolates of staphylococci: an evaluation of three different screening methods. Indian Journal of Medical Microbiology. 2006 Jan 1;24(1):25.
11. Soumya KR, Thomas SA, Sugathan S, Mathew J, Radhakrishnan EK. Antibiotic susceptibility and multiplex PCR analysis of coagulase negative staphylococci isolated from laboratory workers. Int J Curr Microbiol Appl Sci. 2013; 2:266-72.
12. Eng RH, Wang C, Person A, Kiehn TE, Armstrong D. Species identification of coagulase-negative staphylococcal isolates from blood cultures. Journal of Clinical Microbiology. 1982 Mar 1;15(3):439-42.
13. Kawamura Y, Hou XG, Sultana F, Hirose K, Miyake M, Shu SE, Ezaki T. Distribution of Staphylococcus Species among Human Clinical Specimens and Emended Description of Staphylococcus caprae. Journal of Clinical Microbiology. 1998 Jul 1;36(7):2038-42.
14. Jakribettu RP, Mustaq SA, Ashthami VC, Anju MM, Safeera MI. Biofilm: Comparison between the Staphylococcus aureus and coagulase negative staphylococcus species isolated from a rural medical college hospital in North Kerala, India. Int. J. Curr. Microbiol. App. Sci. 2014; 3(1):23-9