Author(s):
Deepthi Yada, T. Sivakkumar, Nimmagadda Srinivas
Email(s):
yada.deepthi@gmail.com
DOI:
10.5958/0974-360X.2021.00074.3
Address:
Deepthi Yada1, Dr. T. Sivakkumar2, Dr. Nimmagadda Srinivas3
1Department of Pharmaceutical Chemistry, Malla Reddy Institute of Pharmaceutical Sciences, Maisammaguda, Dhulapally, Secunderabad-14.TS.
2Department of Pharmacy, Annamalai University, Annamalai Nagar, Chidambaram TN.
3Department of Pharmaceutical Chemistry, Bharat Institute of Technology- Pharmacy, Ibrahimpatnam, Hyderabad.
*Corresponding Author
Published In:
Volume - 14,
Issue - 1,
Year - 2021
ABSTRACT:
The present study was to investigate total phenol and flavonoid content, the antioxidant potential of various extracts of Hyptis suaveolens whole plant using various in vitro systems were quantified by colorimetric methods. The Chloroform extract exhibited potent antioxidant activity as determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH), nitric oxide scavenging and ferric reducing antioxidant power assays (FRAP). The total phenolic content and flavonoid content of chloroform extract of plant was found to 86.16±0.877 and 64.66±1.201mg of GAE and Quercetin equivalents respectively.
Cite this article:
Deepthi Yada, T. Sivakkumar, Nimmagadda Srinivas. Phytochemical Evaluation and in-vitro Antioxidant Potential of Whole Plant of Hyptis suaveolens. Research J. Pharm. and Tech. 2021; 14(1):409-412. doi: 10.5958/0974-360X.2021.00074.3
Cite(Electronic):
Deepthi Yada, T. Sivakkumar, Nimmagadda Srinivas. Phytochemical Evaluation and in-vitro Antioxidant Potential of Whole Plant of Hyptis suaveolens. Research J. Pharm. and Tech. 2021; 14(1):409-412. doi: 10.5958/0974-360X.2021.00074.3 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2021-14-1-74
REFERENCES:
1. Cao YH, Cao RH. Angiogenesis inhibited by drinking tea. Nature. 1999; 398(6726):381.
2. Kahkonen MP, Hopia AI, Rauha JP, Kujala TS et.al., Antioxidant activity of plant extracts containing phenolic compounds. Journal of Agriculture and Food Chemistry.1999; 47:3954–3962.
3. Marnett LJ (2000) Oxyradicals and DNA damage. Carcinogenesis. 2000; 2:361–370.
4. Sastri BN, editors. Wealth of India, New Delhi: CSIR, Vol. V (HK); 1959.
5. Singleton VL, Orthofer R, Lamuela-Raventos RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of Foline Ciocalteu reagent. Methods of Enzymology. 1999; 299:152-78.
6. Chang C, Yang M, Wen H, Chern JC. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of Food Drug Analysis. 2002; 10:178-82.
7. Ilahi I, Samar S, Khan I, Ahmad I. In vitro antioxidant activities of four medicinal plants on the basis of DPPH free radical scavenging. Pakistan Journal of Pharmaceutical Sciences. 2013; 26: 949-952.
8. Garrat, D.C., The Quantitative Analysis of drugs. Chapman and Hall, Japan. Vol (3) ; PP: 456 - 458.
9. Oyaizu, M., Studies on products of browning reactions: Antioxidant activity of products of browning reaction prepared from glucosamine. Japan Journal of Nutrition.1986; 44: 307-315.