Author(s):
Jeenathunisa. N, Rajan. S
Email(s):
jeenathunisa22@gmail.com
DOI:
10.5958/0974-360X.2021.00008.1 
Address:
Jeenathunisa. N1*, Rajan. S2
1Research Scholar, PG and Research Department of Microbiology, M.R. Government Arts College, Mannargudi-614001, Thanjavur (Dt.), Tamil Nadu, India. Affiliated to Bharathidasan University, Tiruchirappalli-620024.
2Assistant Professor, PG and Research Department of Microbiology, M.R. Government Arts College, Mannargudi-614001, Thanjavur (Dt.), Tamil Nadu, India. Affiliated to Bharathidasan University, Tiruchirappalli-620024.
*Corresponding Author
Published In:
Volume - 14,
Issue - 1,
Year - 2021
ABSTRACT:
Asoka-Saraca asoca (Roxb.) de Wilde is one of the traditional plants with well-known medicinal values prescribed widely to treat haematuria, dyspepsia, fever, tumor, burning sensation, visceromegaly, colic, ulcers, menorrhagia, metropathy leucorrhoea, skin infection along with many other pharmacological activities. As globally, we look towards the use of non-toxic plant based products with conventional medicinal usage and the developing countries depends on herbal medicines for their primary health care needs. And so, the aim of this study is to explore the the in vitro cytotoxicity of the bark extracts of Saraca asoca on HT-29 cancer cell line using MTT and SRB assays. The results show a significant inhibition percentage on HT-29 cancer cell line was observed in both assays. In MTT assay the IC50 values was 174.1 and 163.2µg/ml on alcohol and aqueous bark extracts, respectively. And the SRB assay shows the IC50 values 94.76 and 200.5µg/mL on the crude ethanolic and aqueous bark extracts, respectively. Thus, both MTT and SRB assays can be used for cytotoxic screening of Saraca asoca herb towards the development of modern drug.
Cite this article:
Jeenathunisa. N, Rajan. S. In vitro Cytotoxic studies of Saraca asoca bark extracts on HT-29 cancer cell Line. Research J. Pharm. and Tech. 2021; 14(1):42-46. doi: 10.5958/0974-360X.2021.00008.1
Cite(Electronic):
Jeenathunisa. N, Rajan. S. In vitro Cytotoxic studies of Saraca asoca bark extracts on HT-29 cancer cell Line. Research J. Pharm. and Tech. 2021; 14(1):42-46. doi: 10.5958/0974-360X.2021.00008.1 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2021-14-1-8
REFERENCE:
1. WHO (2005). World Health Organization, National Policy on Traditional Medicine and Regulation of Herbal Medicines. Report of WHO global survey, Geneva, Switzerland.
2. Abou-Elella F and R. Mourad R. Anticancer and anti-oxidant potentials of ethanolic extracts of Phoenix dactylifera Musa acuminata and Cucurbita maxima. Research J Pharm Biol Chem Sci. 2015; 6(1): 710-720.
3. Kusari S, Singh S, Jayabaskaran C. Rethinking production of Taxol® (paclitaxel) using endophyte biotechnology. Trends Biotechnol. 2014; 32: 304-311.
4. Li JWH, Vederas JC. Drug discovery and natural products: End of an era or an endless frontier? Science. 2009; 325:161-5.
5. Prashanth R, Tagore S, Adam B. Epidemiology of colorectal cancer: incidence, mortality, survival, and risk factors. Gastroenterology Rev. 2019: 14(2): 89-103.
6. Barretina J, Caponigro G, Stransky N, Venkatesan K, Margolin AA, Kim S, et al. The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity. Nature 2012; 483:603-7.
7. Garnett MJ, Edelman EJ, Heidorn SJ, Greenman CD, Dastur A, Lau KW, et al. Systematic identification of genomic markers of drug sensitivity in cancer cells. Nature 2010; 483:603 7
8. Wilding JL, McGowan S, Liu Y, Bodmer WF. Replication error deficient and proficient colorectal cancer gene expression differences caused by 3′UTR poly T sequence deletions. Proc Natl Acad Sci U S A. 2010; 107:21058–63.
9. Bracht K, Nicholls AM, Liu Y, Bodmer WF. 5-Fluorouracil response in a large panel of colorectal cancer cell lines is associated with mismatch repair deficiency. Br J Cancer 2010; 103:340-6
10. Ashraf SQ, Nicholls AM, Wilding JL, Ntouroupi TG, Mortensen NJ, Bodmer WF. Direct and immune mediated antibody targeting of ERBB receptors in a colorectal cancer cell-line panel. Proc Natl Acad Sci U S A. 2012; 109:21046–51
11. J¨org Weyermanna, Dirk Lochmanna, Andreas Zimmer, A practical note on the use of cytotoxicity assays. Int J Pharm. 2005; 288(2): 369-376.
12. Astalakshmi N and Sundara Ganapathy R. In vitro cytotoxicity studies on methanolic leaf extract of Mussaenda erythrophylla Schumach. and Thonn. Research J Pharm and Tech. 2020; 13(2): 831-839.
13. Merin Babu JJ, Thomas S, Joseph J. Evaluation of cytotoxic activity of Annona muricata fruits and leaves. Research J Pharm and Tech. 2019; 12:3802-3806.
14. H Kim, Yoon, SC, T Y Lee, Jeong D. Discriminative cytotoxicity assessment based on various cellular damages. Toxicology Letter. 2009; 184: 13-17.
15. Solanki N and Harish D. Reverse phase high-performance liquid chromatographic estimation and in vitro cytotoxicity of Boswellic acids on a-375 melanoma cancer cell lines. Asian J. Pharm. Ana. 2018; 8(1): 13-19.
16. Skehan P, Storeng R, Scudiero D, Monks A, McMahon J, Vistica D, Warren JT, Bokesch H, Kenney S, Boyd MR. New colorimetric cytotoxicity assay for anticancer-drug screening. J Natl Cancer Inst. 1990;82(13):1107–1112
17. Firoj A. Tamboli, Harinath N. More. In vitro antipsoriatic study of successive solvent extracts of Barleria gibsoni Dalz. using Ha Ca T keratinocyte cells. Research J Pharm and Tech. 2015; 8(11):1566-1569.
18. Rihana Fathima H and Neelakanta Reddy P. Effect of Asoka bark (Saraca indica) and custard apple pulp (Annona squamosa) on wound healing in female Albino Rats. Research J. Pharm. and Tech. 2011; 4(6): 928-931.
19. Vandana B . Asoka: Herbal boonto gynecological problems an overview of current research. Ayurveda Journal of Health. 2014; 12 (4): 58-6.
20. Karthiga M, Punnagai K, Darling Chellathai D. Cytotoxic effect of ethanolic extracts of Andrographis echioides in human colon cancer cell lines via apoptosis. Research J. Pharm. and Tech. 2020; 13(2):871-876.
21. Ardra Asokan, M. Thangavel. Invitro cytotoxic studies of crude methanolic extract of Saraca indica bark extract. J Pharm Bio Sci. 2014; 9(4):2319-7676.
22. Saranya M, Punnagai K, Darling Chellathai D, Anusha D. Evaluation of Anticancer Effects of Vasopressin Receptor blocker in Colon Cancer – An In vitro Study. Research J. Pharm. and Tech. 2020; 13(1):77-80.
23. Azwanida NN. A review on the extraction methods use in medicinal plants, principle, strength and limitation. J Med Aromat Plants. 2015; 4 (3): 196-201.
24. Odey MO, Iwara IA, Udiba UU, Johnson JT, Inekwe UV, Asenye M. et al. Preparation of plant extracts from indigenous medicinal plants. Int. J Sci. Technol. 2012; 1(12):688-692.
25. Sanganna B, Kulkarni AR. Antioxidant and anti-colon cancer activity of fruit peel of citrus reticulate essential oil on HT-29 cell line. Research J Pharm and Tech. 2013; 6(2):216-219.
26. Hattori N. et al. Enhanced microbial biomass assay using mutant luciferase resistant to benzalkonium chloride. Anal. Biochem. 2003; 319287-95.
27. Vichai V, Kirtikara K. Sulforhodamine B colorimetric assay for cytotoxicity screening. Nat Protoc 2006; 1(3): 1112-1116.
28. Hans Vogel. Drug discovery and evaluation: pharmacological assay. Springer Publisher. 2007:1334.
29. Gomathi D, Ravikumar G, Kalaiselvi M. HPTLC fingerprinting analysis of Evolvulus alsinoides (L.) L. Journal of Acute Medicine. 2012; 2: 77-82.
30. Nistane NT. Herbal nanoparticles against cancer. Res. J. Pharma. Dosage Forms and Tech.2019; 11(4):247-252
31. Benzie IFF and Wachtel-Galor S, editors. Herbal medicine: biomolecular and clinical aspects, Boca Raton (FL): CRC Press/Taylor & Francis. 2011; 2nd ed: Chapter .1
32. Slater T et al. Biochem. Biophys. Acta. 1963; 77:383.
33. van de Loosdrecht, AA et al. J. Immunol. Met. 1994; 174: 311-320.
34. Alley MC, Scudiero DA, Monks A et al. Feasibility of Drug Screening With Panels of Human Tumor Cell Lines Using a Microculture Tetrazolium Assay.Cancer Res. 1988; 48: 589-601.
35. Keepers YP, Pizao PE, Peters GJ, van Ark-Otte J, Winograd B, Pinedo HM. Comparison between SRB and MTT assays for in vitro chemosensitivity testing. Eur. J. Cancer. 1991; 27(7): 897-900.
36. Phang CW, Karsani SA, Adbd Malej SN. Induction of apoptosis and cell cycle arrest by flavokawain C on HT-29 human colon adenocarcinoma via enhancement of reactive oxygen species generation, upregulation of p21, p27 and Gadd153, and inactivation of inhibitor of apoptosis proteins. Phcog Mag. 2017; 13(Suppl S2): 321-8.
37. Priya V, Rao S. Evaluation of anticancer activity of Trideax procumbens leaf extracts on A549 and Hep G2 Cell Lines. Asian J Pharm Clin Res. 2015; 8(3):29-132.