Author(s):
Avinash Birajdar, Varsha Tegeli, Suyash Ingale, Gajanand Nangare
Email(s):
avibirajdar1998@gmail.com
DOI:
10.52711/0974-360X.2021.00951
Address:
Avinash Birajdar*, Varsha Tegeli, Suyash Ingale, Gajanand Nangare
D.S.T.S. Mandal’s College of Pharmacy, Solapur-413004, Maharashtra, India.
*Corresponding Author
Published In:
Volume - 14,
Issue - 10,
Year - 2021
ABSTRACT:
A Reverse Phase High Performance Liquid Chromatographic method has been developed and validated for estimation of Ritonavir in API and Tablet formulation. The chromatographic separation was carried out using ZOROBAX Bonus-RP C-18 column (250 x 4.6mm, 5µm) as stationary phase and Methanol: Acetonitrile: 0.1% Trifluoroacetic acid water (81:9:10) as mobile phase at 1.0 ml/min flow rate and detection was carried out at 250 nm. The method was validated accordance to the Guidelines of International Council for Harmonization (ICH). Ritonavir have linearity in the concentration range of 50-150µg/ml with correlation coefficient (r2=1) respectively. Ritonavir eluted at 3.05 min respectively. The method is sensitive, precise and accurate. So, the method can be successfully applied for the routine analysis of Ritonavir in pharmaceutical formulations.
Cite this article:
Avinash Birajdar, Varsha Tegeli, Suyash Ingale, Gajanand Nangare. Development and Validation of RP-HPLC method for the Estimation of Ritonavir in API and tablet Formulation. Research Journal of Pharmacy and Technology 2021; 14(10):5457-0. doi: 10.52711/0974-360X.2021.00951
Cite(Electronic):
Avinash Birajdar, Varsha Tegeli, Suyash Ingale, Gajanand Nangare. Development and Validation of RP-HPLC method for the Estimation of Ritonavir in API and tablet Formulation. Research Journal of Pharmacy and Technology 2021; 14(10):5457-0. doi: 10.52711/0974-360X.2021.00951 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2021-14-10-69
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