Author(s):
Sugunadevi Govindasamy, Suresh Kathiresan
Email(s):
g.devisuguna@gmail.com
DOI:
10.5958/0974-360X.2021.00147.5 
Address:
Sugunadevi Govindasamy1, Suresh Kathiresan2
1Assistant Professor and Head, Department of Biochemistry, Dhanalakshmi Srinivasan College of Arts and Science for Women (Autonomous), Perambalur -621212.
2Associate Professor, Department of Biochemistry and Biotechnology, Annamalai University, Annamalai Nagar.
*Corresponding Author
Published In:
Volume - 14,
Issue - 2,
Year - 2021
ABSTRACT:
The present study was investigated to judge the immune histochemical expression of inflammatory and angiogenic markers in DMBA induced hamster buccal pouch carcinogenesis. a complete variety of 24 golden Syrian hamsters were randomized into randomised into four teams of six animals in each. Group I animals were served as untreated control. Groups II and III animals were painted with 0.5% DMBA in liquid paraffin three times per week for 14 weeks on the left buccal pouches. Group III animals were orally administered with Mosinone-A (2mg kg-1 b.wt) starting one week before the exposure to the carcinogen and continued on days alternate to DMBA painting, until the sacrification of the animals. Group IV animals were received Mosinone-A alone throughout the experimental period. Topical application of DMBA for 14 weeks induced buccal pouch carcinomas associated with increased expression of, cytokeratin-8 and iNos where as decreased expression of Cytokeratin. Oral administration of Mosinone-A significantly inhibited the development of HBP carcinomas as revealed by decreased expression of, Cytokeratin-8 and iNos increased expression Cytokeratin. The result of this present study indicates that Mosinone-A will exerts protective effects against DMBA induced buccal pouch carcinogenesis. These findings counsel that Mosinone-A exerts its malignant neoplasm properties by inhibiting cell proliferation and inducement differentiation and caspase mediated cell death.
Cite this article:
Sugunadevi Govindasamy, Suresh Kathiresan. Inhibitory Effect of Mosinone - A on Immunohistochemical Expression of Inflammatory and Angiogenic markers in 7,12-dimethylbenz (a) Anthracene induced Hamster Buccal Pouch Carcinogenesis. Research J. Pharm. and Tech. 2021; 14(2):833-837. doi: 10.5958/0974-360X.2021.00147.5
Cite(Electronic):
Sugunadevi Govindasamy, Suresh Kathiresan. Inhibitory Effect of Mosinone - A on Immunohistochemical Expression of Inflammatory and Angiogenic markers in 7,12-dimethylbenz (a) Anthracene induced Hamster Buccal Pouch Carcinogenesis. Research J. Pharm. and Tech. 2021; 14(2):833-837. doi: 10.5958/0974-360X.2021.00147.5 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2021-14-2-43
REFERENCES:
1. Kupferman ME and Myers JN. Molecular Biology of oral cavity squamous cell carcinoma. Otolaryngol Clin North Am. 2006; 39(2): 229-47
2. Kornberg LJ, Villaret D and Popp M, Gene expression profiling in squamous cell carcinoma of the oral cavity shows abnormalities in several signaling pathways. Laryngoscope. 2005;115: 690-98.
3. Gimenez-Conti IB, Slaga TJ, The hamster cheek pouch carcinogenesis Model. J Cell Biochem. 1993; 17: 83-90
4. Jerina DM, Yagi H and Lehr RE. The bay-region theory of carcinogenesis by polycyclic aromatic hydrocarbons. In: Polycyclic aromatic hydrocarbons and cancer. Environment, chemistry and metabolism. New York: Academic Press. 1978.p.173-88.
5. Oshima RG. Apoptosis and keratin intermediate filaments. Cell Death Differ. 2002; 9:486-492.
6. Moll R, Franke WW, Schiller D, Geiger B, Kepler H. The catalog of human Cytokeratin patterns of expression in normal epithelia, tumors and cultured cells. Cell. 1982; 31: 1 l-24.
7. Ambs S, Hussain SP and Harris CC. Interactive effects of nitric oxide and the p53 tumor suppressor gene in carcinogenesis and tumor progression. FASEB J. 1997; 11: 443–48.
8. Hopp DC. Novel mono tetra hydro furan ring acetogenins from the bark of Annona squamosa, showing cytotoxic selectivities for the human pancreatic carcinoma cell line PAC-2. J Nat prod. 1997; 60:581-586.
9. Maclaughlin JL and Hoop DC. Selectivity cytotoxic acetogenin compound. United States patent, US6242483 242 (2004) 423.
10. Kalhor N, Ramirez PT, Deavers MT, Malpica A, Silva EG. Immunohistochemical studies of trophoblastic tumors. Am J Surg Pathol. 2009; 33:633-38.
11. Li P, Nijhawan D, Budihardjo I, Srinivasula SM, Ahmad M, Alnemri ES, Wang X, Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell, . 1997; 91: 479-89.
12. Wilkinson J and Clapper ML. Detoxification enzymes and chemoprevention. Proc Soc Exp Biol Med.1997; 216:192-200.
13. Ragers AE, Zeisel SH and Groopman J. Diet and carcinogenesis. Carcinogenesis. 1993; 14:2205–2217
14. Zeng L. Novel mono tetra hydro furan ring acetogenins from the bark of Annona squamosa, showing cytotoxic selectivities for the certain specific human tumor cell line PAC-2. J Nat prods. 1996; 59:1035-1042.
15. Barak V, Goike H and Panaretakis KW, Clinical utility of cytokeratins as tumor markers. Clin Biochem, 2004;37:529-540.
16. Lopes LF and Bacchi CE, Cytokeratin expression in gastrointestinal stromal tumor: a clinicopathologic and immunohistochemical study of 687 cases. Appl Immunohistochem Mol Morphol, 2012 ;20:8-12.
17. Franzen B, Linder S, Alaiya AA, Eriksson E, Uruy K, Hirano T, Okuzawa K, Auer G. Analysis of polypeptide expression in benign and malignant human breast lesions: down-regulation of cytokeratins. British Journal of Cancer. 1996;74: 1632–638.
18. Raul U, Sawant S, Dange P, Kalraiya R, Ingle A, Vaidya M. Implications of cytokeratin 8/18 filament formation in stratified epithelial cells: induction of transformed phenotype. Int J Cancer. 2004 : 111:662-668.
19. Chu PG and Weiss LM. Keratin expression in human tissues and neoplasms. Histopathology. 2002: 40:403-439.
20. Hansson A, Bloor BK and Sarang Z, Analysis of proliferation, apoptosis and keratin expression in cultured normal and immortalized human buccal keratinocytes. Eur J Oral Sci. 2003: 11:34-41.
21. Knowles RG and Moncada S. Nitric oxide synthases in mammals. Biochem J. 1994; 298: 249-258.
22. Kolb JP, Mechanisms involved in the pro- and anti-apoptotic role of NO in human. Leukemia. 2000; 14: 1685–1694.
23. Maricris Macabeo-Ong, Nusi Dekker, Richard C.K. Jordan, Brian L. Schmidt. Increased nitric oxide levels and iNOS over-expression in oral squamous cell carcinoma. Oral Oncology. 2005; 41: 261-267.