Author(s):
Beena P, Shithin Ann Varghese, Jinny Ann Alexander, Steffy P Raju, Sherin Koshy, Feba Elsa Mathew, Elessy Abraham
Email(s):
shithinv95@gmail.com
DOI:
10.5958/0974-360X.2021.00152.9 
Address:
Dr. Beena P1*, Shithin Ann Varghese2, Jinny Ann Alexander2, Steffy P Raju2, Sherin Koshy2, Feba Elsa Mathew2, Dr. Elessy Abraham3
1Professor, Nazareth College of Pharmacy, Othera P.O Thiruvalla, Kerala, India - 689546.
2M. Pharm Delegate, Nazareth College of Pharmacy, Othera P.O Thiruvalla, Kerala, India - 689546.
3Principal, Nazareth College of Pharmacy, Othera P.O Thiruvalla, Kerala, India - 689546.
*Corresponding Author
Published In:
Volume - 14,
Issue - 2,
Year - 2021
ABSTRACT:
The present research has been undertaken with the aim to develop a transdermal Cubosomal gel formulation of Ketoprofen, which would attenuate the gastrointestinal toxicities associated with oral administration. This research also aimed to encapsulate high drug pay load in Cubosomes for improved therapeutic efficiency. Cubosomes were prepared by Top- down technique. Different formulations (F1 – F9) were prepared and optimized for better performance in terms of drug content, SEM analysis, Zeta potential, entrapment efficiency and drug release. From F1 – F9 formulations, studies showed that F7 is better. Then it is formulated into gel using carbopol as gel base. The physical parameters like appearance, pH, viscosity, spredability, extrudability, ex- vivo drug release and in vitro skin irritation test using HET- CAM, were also evaluated. The cubosomal gel formulation (F7) was found to be clear without any aggregate indicating excellent homogenicity. The pH of the formulation was found close to neutral, indicating the absence of skin irritation. In vitro skin irritation study also reveals there is no skin irritation. The ex-vivo drug release study shows that the formulation (F7) has a good release rate when compared to other topical gels (87.2%). The kinetic study of the optimized formulation (F7) was also carried out and found that the formulation undergo zero order kinetics. The mechanism of drug release was found to be Higuchi model.
Cite this article:
Beena P, Shithin Ann Varghese, Jinny Ann Alexander, Steffy P Raju, Sherin Koshy, Feba Elsa Mathew, Elessy Abraham. Formulation and Evaluation of Cubosomal Gel of an Anti-inflammatory Agent. Research J. Pharm. and Tech. 2021; 14(2):857-862. doi: 10.5958/0974-360X.2021.00152.9
Cite(Electronic):
Beena P, Shithin Ann Varghese, Jinny Ann Alexander, Steffy P Raju, Sherin Koshy, Feba Elsa Mathew, Elessy Abraham. Formulation and Evaluation of Cubosomal Gel of an Anti-inflammatory Agent. Research J. Pharm. and Tech. 2021; 14(2):857-862. doi: 10.5958/0974-360X.2021.00152.9 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2021-14-2-48
REFERENCES:
1. Bhosale RR, Osmani RA, Harkare BR and Ghodake PP. Cubosomes. The Inimitable Nanoparticulate Drug Carriers. Scholars Academic Journal of Pharmacy. 2013; 2(6):481-486.
2. Anbarasan.B, Fatima Grace Shanmuganathn. An overview of Cubosomes: Smart drug delivery system. Sri Ramachandra journal of medicine. 2015; 8(1); 215-220.
3. Ansel, H. C., Popovich, N. G. and Allen, Loyd V., 2005. Pharmaceutical Dosage Forms and Drug Delivery Systems, 415-419.
4. Hundekar et al. Preparation and evaluation of Diclofenac sodium Cubosomes for percutaneous administration. Wjpps 2014;3(5): 523-539.
5. Pawar PD, Arane PM, Saindane HB, Talele SG, Shinde G, Chaudhari G. Review on pharmaceutical excipients. Am. J. Pharm health Res. 2015; 3(2): 32-50.
6. Verma N, Deshwal S. Design and in vitro evaluation of transdermal patches containing Ketoprofen. World J Pharma Res. 2014; 3(3): 3930- 44.
7. Ramani et al. Formulation and evaluation of mephenesin topical gel. World J Pharma Res. 2013; 2(3): 1475-1489.
8. Ben J. B. Characterisation of drug release from cubosomes using the pressure ultrafiltration method. Int J Pharm. April 2003; 260: 239-247.
9. Baboota, S., Al-azaki, A., Kohli, K., Ali, J., Dixit, N., 2007. In vivo assessment ofenhanced topical delivery of econazole nitrate to human stratum corneum, PDA journal of Pharmaceutical science and Technology. 1-10.
10. Indira. S et al. Formulation and evaluation of Aceclofenac loaded Cubosomal topical gel. Ijprs. 2015; 4(4): 27- 37.
11. Gulsu A, Ayhan F, Ayhan H. Preparation and characterization of Ketoprofen loaded albumin microspheres. Turk J Biochem. 2012; 37 (2): 120–128.
12. Indira. S et al. Formulation and evaluation of Aceclofenac loaded Cubosomal topical gel. Ijprs. 2015; 4(4): 27- 37.
13. Soumen Pattanayek et al. Formulation and evaluation of ketoprofen loaded nanoparticulate gel for topical delivery. ijppr.Human, 2018; 11(3): 250-260.
14. Kalyani B. Awale et al. Preparation and evaluation of herbal gel containing Clerodendrum serratum for anti-inflammatory activity. Der Pharmacia Sinica, 2014, 5(4):47-54.
15. Rakesh P. Patel. Formulation and Evaluation of Carbopol Gel Containing Liposomes of Ketoconazole. International Journal Of Applied Pharmaceutics. 2011; 3(3): 1-9.
16. Luzzati V, Husson F. The structure of the liquid-crystalline phases of lipid–water systems. J. Cell Biol. 1962; 12: 207-219.