Author(s): Anu S, Shiburaj S, Prabhakumari C


DOI: 10.5958/0974-360X.2021.00181.5   

Address: Anu S*, Shiburaj S, Prabhakumari C
Department of Biotechnology, CEPCI Laboratory and Research Institute, Kollam-691001, Kerala.
*Corresponding Author

Published In:   Volume - 14,      Issue - 2,     Year - 2021

In the present study 45 chitinsae producing streptomyces were isolated from the soil samples from Agasthyarkoodam region of Westen Ghat, Kerala. Molecular analysis technique together with traditional morphological and biochemical methods was used for the identification of strains and all strains identified as Streptomyces species of this fifteen identified as potent chitinase producers. forty five isolates out of fifteen showed chitinase activity according to a chitinolytic activity test and on colloidal chitin agar plates. Results indicated that TBG AMLA-5 was found to be the most prominent chitinase producer, giving 58 U/ml chitinase activity. The strains observed to have F-19 gene based on the primer designed from conserved regions of Fungal -19 chitinase gene of Streptomyces spp. Based on the 16S rDNA sequence analysis, these isolates were identified as Streptomyces champavatii (TBG AMLA-5). The identity percentages ranged from 97%. Based on the conserved regions among the family 19 chitinase genes of Streptomyces sp. two primers were used for detection of the chitinase (chiC) gene in the five isolates. A DNA fragment of 1.4 kb was observed only for the isolate TBG AMLA-5. In conclusion, five Streptomyces strains with potential chitinolytic activity were identified from the Agasthyaarkoodam region of Western Ghat. Of these isolates, one belong to family 19 chitinases. To our knowledge, this is the first reported presence of a Family19 chi gene of Streptomyces champavatii in this region.

Cite this article:
Anu S, Shiburaj S, Prabhakumari C. PCR-based screening and characterization of family-19 chitinase producing Streptomyces sp. from western Ghats forest soil. Research J. Pharm. and Tech. 2021; 14(2):1017-1021. doi: 10.5958/0974-360X.2021.00181.5

Anu S, Shiburaj S, Prabhakumari C. PCR-based screening and characterization of family-19 chitinase producing Streptomyces sp. from western Ghats forest soil. Research J. Pharm. and Tech. 2021; 14(2):1017-1021. doi: 10.5958/0974-360X.2021.00181.5   Available on:

1.    Priya CS. Jaganathan N. Kalaichelvan PT. 2011. Production of chitinase by Streptomyces hygroscopicus VMCH2 by optimization of cultural conditions. International Journal of Pharma and Bio Sciences. 2, 210-219.
2.    Anderson SA. Wellington EM. H. 2001. The taxonomy of Streptomyces and related genera. Inter. J. Sys.Evo. Microbiol.51, 797-814.
3.    Senol M. Nadaroglu H. Dikbas N. Kotan R. 2014.Purification of Chitinase enzymes from Bacillus subtilis bacteria TV-125, investigation of kinetic properties and antifungal activity against Fusarium culmorum. Annals of Clinical Microbiology and Antimicrobials. 13,35.
4.    Henrissat B.1991. A classification of glycosyl hydrolases based on amino acid sequence similarities. Biochem. J. 280, 309-316.
5.    Henrissat B. Bairoch A. 1993. New families in the classification of glycosyl hyrolases based on amino acid sequence similarities. Biochem. J. 293, 781-788.
6.    Davies G. Henrissat B. 1995. Structures and mechanisms of glycosyl hydrlases. Structure 3, 853-859.
7.    Ohno T. Armand S. Hata T. Nikaidou N. Henrissat B. Mitsutomi M. and Watanabe T. 1996. A modular family 19 chitinase found in the prokariyotic organism Streptomyces griseus HUT 6037. J. Bacteriol. 178, 5065-5070.
8.    Murray MG. Thompson WF. 1980. Rapid isolation of high molecular weight plant DNA. Nucl. Aci. Res. 8, 4321-4325.
9.    Thompson JD. Gibson TJ. Plewniak F. Jeanmougin F. and Higgins DG. 1997. The Clustal X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res.24, 4876-4882.
10.    Kimura M. 1980. A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. J Mol Evol. 16, 111-120.
11.    Saitou N. Nei M. 1987. The neighbor- joining method : a new method for reconstructing phylogenetic trees. Mol.Biol.Evol.4, 406-425.
12.    Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein- dye binding. Anal. Biochem. 72, 248-254.
13.    Miller GL.1959. Use of dinitrosalicylic acid reagent for determination of reducing sugars. Anal. Chem.31, 426-428.
14.    Pridham TG. Gottlieb D. 1948. The utilization of carbon compounds by some actinomycetales as an aid for species determination. Journal of Bacteriology. 56, 107-114.
15.    Shirling EB. Gottlieb D.1966.Methods for characterization of Streptomyces species. Inst. J. Syst. Bacteriol. 16, 313-340.
16.    Park HS. Jhon J. Kilbane II. 2006. Rapid detection and high- resolution discrimination of the genus Streptomyces based on 16S rRNA spacer region and denaturing gradient gel electrophoresis. J. Ind. Microbiol. Biotechnol. 33, 289-297.
17.    Narayana, K.J.P and Vijayalekshmi, M.(2008).Production of extracellular α-amylase by Streptomyces albidofavus. Asian.J..Biochem.,3:194-197.
18.    Kim K. 2001. Effect of chitin sources on production of chitinase and chitosanase by Streptomyces griseus HUT 6037. Biotechnol Bioprocess Eng.6, 18-24.
19.    Watanabe T. Kanai R. Kawase T. Misutomi M. Sakuda S. and Miyashita K.1999. Family19 chitinase of Streptomyces species : characterization and distribution . Microbiology. 145, 3353-3363.
20.    Kawase T. Saito A. Sato T. Kanai R. Fujii T. Nikaidou N. Miyashita K. and Watanabe T. 2004. Distribution and phylogenetic analysis of family 19 chitinases in Actinobacteria. Appl. Environ. Microbiol.70, 1135-1144.

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