ABSTRACT:
Chlorpheniramine Maleate (CPM) and Glyceryl Guaiacolate (GUA) are the ß-lactum antibiotic drug. Sensitive, precise, accurate and simple, UV spectrophotometric methods have been developed for the simultaneous estimation of Chlorpheniramine Maleate (CPM) and Glyceryl Guaiacolate (GUA) in dosage form. Two spectrophotometric methods (simultaneoue equations and Q-Absorbance ratio) were applied for the determination of the drugs as mixture. The maximum absorbance of drug in solvent mixture composed of water – acetontrile – methanol in a ratio of (80% H2O – 10% ACN – 10% MOH) was found to be at (261.4 nm and 273 nm) for CPM, GUA respectively, and the Q – isosbestic point was found at 270.4 nm. These wavelengths were selected for the analysis of drugs as mixtures standard and in the manufactured samples using the two developed methods. The methods were linear in the range of (1- 100) µg/mL for (CPM, GUA), with an R2 of (0.9996) for CPM and GUA respectively in the mixture. Recovery means were found to be (99.79 % - 100.30 %) for the standard drugs CPM and GUA respectively and in formulating drugs was found to be (99.71 – 100.41 %). LOD and LOQ were established and found to be (0.1 and 0.33) for CPM and GUA respectively. The method was applied for the estimation of the active gradient of the drugs in different samples of manufactured dosage. The accuracy of method was validated by mean percentage recovery, which was found to be in the acceptable range.
Cite this article:
Nada Ghaaeb Hussein, Ahmed Mahdi Saeed. Simultaneous Estimation of Chlorpheniramine Maleate and Glyceryl Guaiacolate in Pure and Capsule Dosage form by using different Spectrophotometric Methods. Research Journal of Pharmacy and Technology. 2021; 14(5):2608-2. doi: 10.52711/0974-360X.2021.00459
Cite(Electronic):
Nada Ghaaeb Hussein, Ahmed Mahdi Saeed. Simultaneous Estimation of Chlorpheniramine Maleate and Glyceryl Guaiacolate in Pure and Capsule Dosage form by using different Spectrophotometric Methods. Research Journal of Pharmacy and Technology. 2021; 14(5):2608-2. doi: 10.52711/0974-360X.2021.00459 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2021-14-5-43
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