Author(s):
A Rajasekaran, V Abirami, S Arunkumar, G Bharani Priya, S Gugapriya, R Arivukkarasu
Email(s):
rsekaran2001in@yahoo.co.in
DOI:
10.52711/0974-360X.2021.00460 
Address:
A Rajasekaran*, V Abirami, S Arunkumar, G Bharani Priya, S Gugapriya, R Arivukkarasu
Department of Pharmaceutical Analysis, KMCH College of Pharmacy, Coimbatore.
*Corresponding Author
Published In:
Volume - 14,
Issue - 5,
Year - 2021
ABSTRACT:
Cardiospermum halicacabum is one of the most potent medicinal plants used in Indian traditional systems of medicine for the treatment of various diseases, mainly for arthritis. Apigenin is one of the major constituent present in Cardiospermum halicacabum. The present study mainly aimed to estimate the content of major constituent apigenin present in Cardiospermum halicacabum collected from 21 districts of Tamil Nadu by HPTLC method using the marker compound apigenin. The HPTLC method was performed using HPTLC aluminium sheets precoated with Silica Gel 60 GF254 as stationary phase and Toluene: Ethyl acetate: formic acid: methanol (3:6:1.6:0.4 v/v) as the mobile phase. The developed chromatogram was scanned at 254nm using Camag Scanner III. The Rf value of standard apigenin and apigenin in the leaf extract of Cardiospermum halicacabum was found to be in the range of 0.80 to 0.89. Plant collected from Cuddalore district of Tamil Nadu was found to contain relatively high amount of marker compound apigenin than other regions.
Cite this article:
A Rajasekaran, V Abirami, S Arunkumar, G Bharani Priya, S Gugapriya, R Arivukkarasu. A Study on the variation of Apigenin content in Cardiospermum halicacabum from 21 districts of Tamil Nadu by HPTLC. Research Journal of Pharmacy and Technology. 2021; 14(5):2613-8. doi: 10.52711/0974-360X.2021.00460
Cite(Electronic):
A Rajasekaran, V Abirami, S Arunkumar, G Bharani Priya, S Gugapriya, R Arivukkarasu. A Study on the variation of Apigenin content in Cardiospermum halicacabum from 21 districts of Tamil Nadu by HPTLC. Research Journal of Pharmacy and Technology. 2021; 14(5):2613-8. doi: 10.52711/0974-360X.2021.00460 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2021-14-5-44
REFERENCES:
1. R.N. Chopra, S.L. Nayar and I.C. Chopra. Glossary of Indian Medicinal Plants, New Delhi; Council for Scientific Industrial Research. 1980; 51-55.
2. S.G. Newmaster, S. Ragupathy, R.F. Ivanhoff. and C.B. Nirmala. Mechanisms of Ethnobiological Classification. Ethnobotany. 2006; 18: 4-26.
3. A. Kumaran, R.J. Karunakaran. Antioxidant activities of the methanol extract of Cardiospermum halicacabum. Pharmaceutical Biology. 2006; 44: 146–151.
4. K.C. Venkatesh Babu, S. Krishnakumari, Cardiospermum halicacabum suppresses the production of TNF-α and NO by human peripheral blood mononuclear cells. African Journal of Biomedical Research. 2006; 9: 95–99.
5. S. Ragupathy, SG Newmaster, G Paliyath, CB Newmaster. Exploring ethnobiological classifications for novel alternative medicine: A case study of Cardiospermum halicacabum L. (Modakathon, balloon vine) as a traditional herb for treating rheumatoid arthritis. Ethnobotany. 2007; 19: 1–20.
6. Balick, M.J., Cox, P.A. Plants, People and Culture: The Science of Ethnobotany. Journal of Natural Products. 1997; 60(4): 428-429.
7. Ghorbani.A. Studies on Pharmaceutical Ethnobotany in the region of Turkmen Sahra, North of Iran (part-1) Journal of Ethnopharmacology. 2005; 102(1): 58-68.
8. Indian Pharmacopoeia, Govt. of India, Ministry of Health and Family welfare, Alimentary Pharmacology and Therapeutics, 1996.
9. WHO Quality Control Methods for Medicinal Plant Materials. AITBS Publishers and Distributors, New Delhi. 1998.