Cardiospermum halicacabum is conventionally used in the treatment of stiffness of limbs, chronic bronchitis, and snakebites. The whole plant of Cardiospermum halicacabum has been extracted using ethanol and aqueous solvent and estimation of their antioxidant activity done for predicted current research work. Both ethanol and aqueous extracts illustrate important antioxidant activity in the DPPH method, nitric oxide method, and hydroxyl radical-induced In-vitro assay method. The DPPH radical inhibition (%) was 53.13, 57.13, and 79.12 for EECH, AECH, and Standard ascorbic acid respectively. The Cardiospermum halicacabum extracts (EECH and AECH) showed significant free radical scavenging action against nitric oxide (NO) induced release of free radicals at the concentrations 250µg/ml, showing 29.22% and 63.32% of nitric oxide inhibition, respectively. The EECH and AECH extracts (25-400µg/ml) significantly scavenged the hydroxyl radical generated by the EDTA/HO system, when compared to that of ascorbic acid. The plant Cardiospermum halicacabum has been used to treat different ailments. The present study clearly showed that both the extracts viz. EECH and AECH of the plant possess significant antioxidant activity. Moreover, ethanolic extract was proved as more potent candidature as compared to aqueous extract. The molecular level mechanism is the future interest of the researchers to prove the traditional use of the plant.
Cite this article:
M.V. Kumudhavalli, V. Nandhinipriya. A Comparative Study of Antioxidant Activity between Ethanolic and Aqueous Extract of Cardiospermum halicacabum. Research Journal of Pharmacy and Technology. 2021; 14(9):4715-8. doi: 10.52711/0974-360X.2021.00820
M.V. Kumudhavalli, V. Nandhinipriya. A Comparative Study of Antioxidant Activity between Ethanolic and Aqueous Extract of Cardiospermum halicacabum. Research Journal of Pharmacy and Technology. 2021; 14(9):4715-8. doi: 10.52711/0974-360X.2021.00820 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2021-14-9-34
1. Anonymous (1992): The Wealth of India—A Dictionary of Indian Raw Materials and Industrial Products, Raw Materials Vol. 3. New Delhi, CSIR, pp. 269–271
2. Kirthikar KR, Basu BD, An ICS (1969): Indian Medicinal Plants, Vol. 1, 2nd ed. New Delhi, Periodical Experts, p. 623.
3. V. Velmurugan, T. Sundarrajan, A. Chandran, G. Arunachalam. Evaluation of Diuretic Activity on Leaves Extract of Cardiospermum halicacabum Linn. Research J. Pharm. and Tech. 2019; 12(4): 1607-1609.
4. Vandana Gautam, Dhriti Kapoor, Saroj Arora, Renu Bhardwaj. Characterization and Antioxidant Activity of oil Extract of a Gymnosperm - Araucaria cunninghamii Aiton ex D. Don. Research J. Pharm. and Tech. 2016; 9(7): 875-879.
5. Krishna Kondragunta. V, Karuppuraj. V, Perumal. K. Antioxidant activity and Folic acid content in indigenous isolates of Ganoderma lucidum. Asian J. Pharm. Ana. 2016; 6(4): 213-215.
6. Priyanga Rangasamy, Vadakkenchery Salimudheen Hansiya, Palanisamy Uma Maheswari, Thamburaj Suman, Natesan Geetha. Phytochemical Analysis and Evaluation of In vitro Antioxidant and Anti-urolithiatic Potential of various fractions of Clitoriaternatea L. Blue Flowered Leaves. Asian J. Pharm. Ana. 2019; 9(2): 67-76.
7. Sherin A. Hameed, Joyamma Varkey, P. Jayasekhar. Schiff bases and Bicyclic derivatives comprising 1, 3, 4-thiadiazole moiety- A Review on their Pharmacological activities. Asian J. Pharm. Res. 2019; 9(4): 299-306.
8. Shivani Sharma, Manpreet Rana, Hitesh Kumar, Bharat Parashar. It’s era to move towards nature for getting beneficial effects of plants having Antioxidant activity to fight against deleterious diseases. Asian J. Pharm. Res. 3(2): April- June 2013; Page 103-106.
9. 9.Ghanshyam B. Jadhav, Ravindranath B. Saudagar. Free radical Scavenging and Antioxidant Activity of Punicagranatum Linn. Asian J. Res. Pharm. Sci. 4(2): April-June 2014; Page 51-54.
10. 10.Amit Roy, Dayananda Bhoumik, Ram Kumar Sahu, Jaya Dwivedi. Phytochemical Screening and Antioxidant Activity of Sesbania grandiflora Leaves Extracts. Asian J. Res. Pharm. Sci. 4(1): Jan.-Mar. 2014; Page 16-21.
11. 11.A. Rajasekaran, R. Arivukkarasu, Joyce Mathew P, Raja Bonagiri, Ramadasu Partha Saradhi. Antimicrobial evaluation and quantification of apigenin content by HPTLC in methanol stem extract of Cardiospermum halicacabum L. Research J. Pharm. and Tech. 7(5): May, 2014; Page 537-543
12. 12.Nitin H. Indurwade, Sunil B. Chaudhari, Rina G. Maskare, Juhi U. Bandre, Nikhil Bhivgade. Comparative Study of Antimicrobial and Antioxidant Activity between Seeds and Leaves of Crude Drug Moringa oleifera. Research J. Pharm. and Tech. 2019; 12(4): 1843-1848.
13. 13.Shashi Singh, Swati R. Dhande, Sneha M. Aggarwal, Avinash Suryawanshi, Vilasrao Kadam. In vitro Antioxidant Activity of 70% Methanolic Extracts of Roots of Hemidesmus indicus. Research J. Pharm. and Tech. 5(9): September 2012; Page 1241-1245.
14. 14.Dima Al Diab, Nour Al Asaad. Comparative Analysis of Ascorbic Acid Content and Antioxidant Activity of Some Fruit Juices in Syria. Research J. Pharm. and Tech 2018; 11(2): 515-520.
15. 15.Huda K. Al-Bazaz, Ibrahim S. Al-jubori, Rasha Eldalawy, Noor M. Nasser. Antimicrobial and Antioxidant Activity Pinus halepensis Miller. Cone Extract which grown in Iraq. Research J. Pharm. and Tech 2018; 11(11): 4977-4980.
16. 16.Pradeep Kumar Samal. Evaluation of Antioxidant activity of Ardisia solanacea in albino rats. Research J. Pharm. and Tech. 6(5): May 2013; Page 585-588.
17. 17.Rekha Rajendran, R Hemachander, T Ezhilarasan, C Keerthana, DL Saroja, KV Saichand, Mohamed Gasim Abdullah. Phytochemical Analysis and In-Vitro Antioxidant Activity of Mimosa pudica Lin., Leaves. Research J. Pharm. and Tech. 3(2): April- June 2010; Page 551-555.
18. 18.Andana Gautam, Dhriti Kapoor, Saroj Arora, Renu Bhardwaj. Characterization and Antioxidant Activity of oil Extract of a Gymnosperm - Araucaria cunninghamii Aiton ex D. Don. Research J. Pharm. and Tech. 2016; 9(7): 875-879.
19. T. Yokozawa, C. P. Chen, E. Dong, T. Tanaka, G. I. Nonaka, I. Nishioka. Study on the inhibitory effect of tannins and flavonoids against the 1, 1- diphenyl-2picrylhydrazyl radical. Biochemical Pharmacology 1998, 56: 213-222.
20. E. M. Conner, M. B. Grisham. Inflammation, Free Radicals and Antioxidants Nutrition; 1996, 12:274.
21. M. Comporti. Three models of free radical induced cell injury, Chem Biol Interact; 1989, 72:1-56.
22. J. M. C. Gutteridge, Age pigments and free radicals; Fluorescent lipid complexes formed by iron and copper containing proteins, Biochem Biophys Acta; 1985, 834:144.
23. K. Z. Guyton, M. Gorospe, N. J. Holbrook. Oxidative stress and the molecular biology of antioxidant defenses, Cold Spring Harbor laboratory Press, New York; 1997, 242-272.
24. S. Bolton. In Pharmaceutical Statistics-Practical and Clinical Applications. New York: Marcel Dekker; 1997
25. Babu KV, Krishnakumari S. Anti-inflammatory and antioxidant compound, rutin in Cardiospermum halicacabum leaves. Ancient Science of Life. 2005; 25(2): 47.
26. Mani RP, Awanish P, Shambaditya G, Poonam T, Kumudhavalli V, Pratap SA. Phytochemical Screening and In-vitro Evaluation of Antioxidant Activity and Antimicrobial Activity of the Leaves of Sesbaniasesban (L) Merr. Free Radicals and Antioxidants. 2011; 1(3): 66-9.
27. Uttara B, Singh AV, Zamboni P, Mahajan RT. Oxidative stress and neurodegenerative diseases: a review of upstream and downstream antioxidant therapeutic options. Current Neuropharmacology. 2009; 7(1): 65-74.
28. Mittal M, Siddiqui MR, Tran K, Reddy SP, Malik AB. Reactive oxygen species in inflammation and tissue injury. Antioxidants and Redox Signaling. 2014; 20(7): 1126-67.
29. Gangwar M, Gautam MK, Sharma AK, Tripathi YB, Goel RK, Nath G. Antioxidant capacity and radical scavenging effect of polyphenol rich Mallotusphilippenensis fruit extract on human erythrocytes: an in vitro study. The Scientific World Journal. 2014; 2014.
30. Kedare SB, Singh RP. Genesis and development of DPPH method of antioxidant assay. Journal of Food Science and Technology. 2011 ;48(4): 412-22.