Author(s): Sahar Amer Ali, Hussein O. M. Al-Dahmoshi

Email(s): amersahar575@gmail.com , dr.dahmoshi83@gmail.com

DOI: 10.52711/0974-360X.2022.00765   

Address: Sahar Amer Ali, Hussein O. M. Al-Dahmoshi*
Biology Department, College of Science, University of Babylon, Iraq.
*Corresponding Author

Published In:   Volume - 15,      Issue - 10,     Year - 2022


ABSTRACT:
Background: Klebsiella pneumoniae can be defined as one of the clinically relevant pathogens that is a common cause of community-acquired and hospital-acquired urinary tract infections (UTI). Objective: The current study was conducted to investigate most common members of 5 classes of efflux pumps among K. Pneumonia isolates. Methodology: K. Pneumonia isolates was diagnosed on EMB and confirmed by tyrB gene. Antibiotic susceptibility test has been done based on the CLSI-2019. Efflux pumps genes were examined via PCR. Results: All isolates were high resist to ceftazidime, Amoxicillin, cefotaxime, ceftriaxone, Cefixime, cefepime, streptomycin and trimethoprime. Moderate resistance were showed to nitrofurantion, Aztreonam, Kanamycin, Cefoxitin, Gentamycine and Tobramycine. Low resistance was exhibited to Ciprofloxacine, Azithromycin, Doxycycline, piperacillin, Nalidixic acid, Imipenem and Amikacine. High sensitivity were exploited to Levofloxacine, ofloxacine, Meropenem and Netilamicin Concern antibiotic resistance patterns PDR, XDR and MDR), the results revealed that (10%) isolates were non-MDR while MDR compile (90%). Results of molecular investigation of efflux pumps in K pneumonia revealed that, AcrAB-TolC, AcrAD-TolC and AcrFE-TolC genes, EmrAB-TolC, EmrD and MdfA, EmrE, YnfA and TehA, MacAB-TolC and MdlAB-TolC, MdtK and DinF) genes were investigated for K. pneumoniae. Results of biofilm formation revealed that 100% were biofilm former (40% weak biofilm, 44% moderate and 16% strong biofilm former). Conclusion: The study concludes that, all efflux pumps may be highly associated with resistance to penicillins and cephalosporins and moderately with streptomycin, trimethoprime, nitrofuraniton, Aztreonam, Kanamycin. Additionally, biofilm formation was highly related to presence of studied pumps.


Cite this article:
Sahar Amer Ali, Hussein O. M. Al-Dahmoshi. Molecular study on Efflux pumps of Klebsiella pneumonia Isolated from patients with Cystitis. Research Journal of Pharmacy and Technology 2022; 15(10):4559-4. doi: 10.52711/0974-360X.2022.00765

Cite(Electronic):
Sahar Amer Ali, Hussein O. M. Al-Dahmoshi. Molecular study on Efflux pumps of Klebsiella pneumonia Isolated from patients with Cystitis. Research Journal of Pharmacy and Technology 2022; 15(10):4559-4. doi: 10.52711/0974-360X.2022.00765   Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2022-15-10-38


REFERENCES:
1.    Levison ME, Kaye D. Treatment of complicated urinary tract infections with an emphasis on drug-resistant gram-negative uropathogens. Curr Infect Dis Rep. 2013; 152): 109-15. doi: 10.1007/s11908-013-0315-7, PMID 23378123.
2.    Kumar MS, Arunagirinathan N, Ravikumar M. Antibiotic susceptibility profile of extended spectrum β-lactamase producing Escherichia coli, Klebsiella pneumoniae and Klebsiella oxytoca from Urinary tract infections. Research Journal of Pharmacy and Technology. 2021; 14(8):4425-8.
3.    Samer A. MH. Al-Hilali, Zainab Jaber Hadi, Kreem G. Aljayashi. Prevalence of Plasmid-mediated quinolone resistance genes among Ciprofloxacin-nonsusceptible Escherichia coli and Klebsiella pneumoniae isolated from clinical isolates in Najaf, Iraq. Research Journal of Pharmacy and Technology. 2021; 14(4):1966-2.
4.    Hooton TM. Clinical practice. Uncomplicated urinary tract infection. N Engl J Med. 2012; 366(11): 1028-1037. doi: 10.1056/NEJMcp1104429, PMID 22417256.
5.    Bao J, Wu N, Zeng Y, Chen L, et al. Non- active antibiotic and bacteriophage synergism to successfully treat recurrent urinary tract infection caused by extensively drug-resistant Klebsiella pneumoniae. Emerg Microbes Infect. 2020; 9(1): 771-774. doi: 10.1080/22221751.2020.1747950,
6.    Anes J, McCusker MP, Fanning S, Martins M. The ins and outs of RND efflux pumps in Escherichia coli. Front Microbiol. 2015; 6: 587. doi: 10.3389/fmicb.2015.00587.
7.    Neupane S, Pant ND, Khatiwada S, Chaudhary R, Banjara MR. Correlation between biofilm formation and resistance toward different commonly used antibiotics along with extended spectrum beta lactamase production in uropathogenic Escherichia coli isolated from the patients suspected of urinary tract infections visiting Shree Birendra Hospital, Chhauni, Kathmandu, Nepal. Antimicrob Resist Infect Control. 2016; 5(1): 1-5.
8.    Shaymaa Husham Ahmed, Rand R. Hafidh. The Isolation of specifically lytic phages along with their extracted endolysins as antibacterial agents to MDR Enterococcus faecalis. Research Journal of Pharmacy and Technology. 2021; 14(9):4547-4.
9.    Jeong ES, Lee KS, Heo SH, Seo JH, Choi YK. Rapid identification of Klebsiella pneumoniae, Corynebacterium kutscheri, and Streptococcus pneumoniae using triplex polymerase chain reaction in rodents. Exp Anim. 2013;62(1):35-40. doi: 10.1538/expanim.62.35.
10.    https://clsi.org/
11.    Helaly GF, Shawky S, Amer R, El Sawaf G, El Kholy MA. Expression of Acrab efflux pump and role of mefloquine as efflux pump inhibitor in MDR E. coli. Am J Infect Dis. 2016; 4(1): 6-13. doi: 10.12691/ajidm-4-1-2.
12.    Prasanthi Boddu, Vijaya Ratna Jayanti. Glyceryl Monooleate Functionalized Matrix with an Inter Polyelectrolyte Complex System for the Management of Drug Resistance in Tuberculosis. Research J. Pharm. and Tech. 2020; 13(10):4839-4850.
13.    Yasufuku T, Shigemura K, Shirakawa T, Matsumoto M, et al. Correlation of overexpression of efflux pump genes with antibiotic resistance in Escherichia coli Strains clinically isolated from urinary tract infection patients. J Clin Microbiol. 2011; 49(1): 189-194. doi: 10.1128/JCM.00827-10.
14.    Mousa JJ, Bruner SD. Structural and mechanistic diversity of multidrug transporters. Nat Prod Rep. 2016; 33(11): 1255-1267. doi: 10.1039/c6np00006a.
15.    Nikaido H, Zgurskaya HI. AcrAB and related multidrug efflux pumps of Escherichia coli. J Mol Microbiol Biotechnol. 2001; 3(2): 215-218.
16.    Ma D, Cook DN, Alberti M, Pon NG, et al. Molecular cloning and characterization of acrA and acrE genes of Escherichia coli. J Bacteriol. 1993; 1751(9): 6299-12313. doi: 10.1128/jb.175.19.6299-6313.1993.
17.    T. Sharanya Nair, Meghana R, Shlini P.Antimicrobial Activity of the protein fraction obtained in the extraction of Curcumin. Asian J. Research Chem. 2019; 12(4):199-202.
18.    Chetri S, Dolley A, Bhowmik D, Chanda DD, et al. Transcriptional response of AcrEF-TolC against fluoroquinolone and carbapenem in Escherichia coli of clinical origin. Indian J Med Microbiol. 2018; 36(4): 537-540. doi: 10.4103/ijmm.IJMM_18_308.
19.    Heacock-Kang Y, Sun Z, Zarzycki-Siek J, Poonsuk K, McMillan IA, Chuanchuen R, Hoang TT. Two regulators, PA3898 and PA2100, modulate the Pseudomonas aeruginosa multidrug resistance MexAB-OprM and EmrAB efflux pumps and biofilm formation. Antimicrob Agents Chemother. 2018; 62(12): e01459-18  doi: 10.1128/AAC.01459-18.
20.    Gurudharshini Natarajan, Madhumitha Muthusamy, Muthusaravanan Sivaramakrishnan, Perianayaki Periasamy, Poornimmashree A, Kumaravel Kandaswamy. A Big Picture on Antimicrobial Strategies then and now. Research J. Engineering and Tech. 2017; 8(4): 361-364.
21.    Nikaido H. Multidrug resistance in bacteria. Annu Rev Biochem. 2009;78:119-46. doi: 10.1146/annurev.biochem.78.082907.145923, PMID 19231985.
22.    Schweizer HP. Efflux as a mechanism of resistance to antimicrobials in Pseudomonas aeruginosa and related bacteria: unanswered questions. Genet Mol Res. 2003;21):48-62. PMID 12917802.
23.    Bay DC, Rommens KL, Turner RJ. Small multidrug resistance proteins: a multidrug transporter family that continues to grow. Biochim Biophys Acta. 2008;17789):1814-38. doi: 10.1016/j.bbamem.2007.08.015.
24.    Jeya KR, VeeraPagu M. Screening for the Antimicrobial Activity of Lippia nodiflora Leaf Extract Against Selective Bacterial Population. Research J. Pharm. and Tech. 4(11): Nov. 2011; Page 1669-1672.
25.    Schuldiner S. EmrE, a model for studying evolution and mechanism of ion-coupled transporters. Biochim Biophys Acta. 2009; 1794(5): 748-762. doi: 10.1016/j.bbapap.2008.12.018.
26.    Hus N. ynfA, a novel Escherichia coli gene of the small multidrug resistance superfamily. University of Miami; 2005.
27.    Modali SD, Zgurskaya HI. The periplasmic membrane proximal domain of MacA acts as a switch in stimulation of ATP hydrolysis by MacB transporter. Mol Microbiol. 2011; 81(4): 937-951. doi: 10.1111/j.1365-2958.2011.07744.x.
28.    S. Niveditha, S.S.M. Umamageswari, D. Aruna, M. Kalyani. Study of Hand Carriage of Multi drug resistant bacteria using Glove Juice Technique in Health Care Workers. Research J. Pharm. and Tech. 2021; 14(2):650-656.
29.    Wasfi R, Elkhatib WF, Ashour HM. Molecular typing and virulence analysis of multidrug resistant Klebsiella pneumoniae clinical isolates recovered from Egyptian hospitals. Sci Rep. 2016: 6(1): 38929. doi: 10.1038/srep38929.
30.    Taghadosi R, Shakibaie MR, Masoumi S. Biochemical detection of N-Acyl homoserine lactone from biofilm-forming uropathogenic Escherichia coli isolated from urinary tract infection samples. Rep Biochem Mol Biol. 2015; 3(2): 56-61.
31.    Mustafa Jawad, Kadhim Hashim, Abbas Mahmood. Prevalence of Anti-Viral Drug Resistance Chronic Hepatitis B patients (CHB) in Iraq. Research J. Pharm. and Tech 2018; 11(8): 3503-3508.
32.    Jafarzadeh Samani R, Tajbakhsh E, Momtaz H, Kabiri Samani M. Prevalence of Virulence Genes and Antibiotic Resistance Pattern in Enterococcus Faecalis Isolated from Urinary Tract Infection in Shahrekord, Iran. Rep Biochem Mol Biol. 2021; 10(1): 50-59. doi:10.52547/rbmb.10.1.50
33.    Prakash B, Veeregowda BM, Krishnappa G. Biofilms: a survival strategy of bacteria. Curr Sci. 2003: 1299-307.
34.    Hisham A. Abbas, Ashraf A. Kadry, Ghada H. Shaker, Reham M. Goda. Resistance of Escherichia coli and Klebsiella pneumoniae isolated from different Sources to β-lactam Antibiotics. Research J. Pharm. and Tech. 2017; 10(2): 589-591.
35.    Naparstek L, Carmeli Y, Navon-Venezia S, Banin E. Biofilm formation and  susceptibility to gentamicin and colistin of extremely drug-resistant KPC-producing Klebsiella pneumoniae. J Antimicrob Chemother. 2014; 69(4): 1027-1034. doi: 10.1093/jac/dkt487.

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