Author(s):
Vidhi S. Dave, Paresh. U. Patel
Email(s):
vidhidave1988@gmail.com
DOI:
10.52711/0974-360X.2022.00778 
Address:
Vidhi S. Dave*, Paresh. U. Patel
Department of Quality Assurance, S. K. Patel College of Pharmaceutical Education and Research, Ganpat University, Ganpat Vidyanagar, Kherva, Mehasana, Gujarat.
*Corresponding Author
Published In:
Volume - 15,
Issue - 10,
Year - 2022
ABSTRACT:
A simple, precise and sensitive UV spectrophotometric method has been developed for the estimation of Canagliflozin and metformin in bulk and pharmaceutical dosage Form by Q Absorbance ratio method. Absorbance uses the ratio of absorbance at two selected wavelengths, one which is an iso absorptive point and other is the ?max of one of the two components. From the overlay spectra of two drugs, it is evident that Canagliflozin and Metformin show an isoabsorptive point at 260nm. The second wavelength used is 235nm which is ?max of Meformin HCl. Beer’s law was obeyed in range of 2-10µg/ml for both Canagliflozin and Metformin HCl. The correlation coefficient was found to be nearer to 0.9990 at both the wavelength for Canagliflozin and Metformin HCl. The result of interday and intraday precision shows standard deviation less than 2% for three concentration and three replicates. The accuracy found to be between 98% - 102%. The LOD and LOQ were found to be within the Linearity Range. The purposed method was novel and can apply for the determination of Canagliflozin and Metformin HCl in Tablet Dosage Form. The method was successfully validated as per the ICH guidelines.
Cite this article:
Vidhi S. Dave, Paresh. U. Patel. Development and Validation of Simultaneous Estimation of Canagliflozin and Metformin by Q Absorbance Ratio Method in its API and Tablet Formulation. Research Journal of Pharmacy and Technology 2022; 15(10):4637-0. doi: 10.52711/0974-360X.2022.00778
Cite(Electronic):
Vidhi S. Dave, Paresh. U. Patel. Development and Validation of Simultaneous Estimation of Canagliflozin and Metformin by Q Absorbance Ratio Method in its API and Tablet Formulation. Research Journal of Pharmacy and Technology 2022; 15(10):4637-0. doi: 10.52711/0974-360X.2022.00778 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2022-15-10-51
REFERENCES:
1. https.//www.drugbank.ca/drugs/DB12935, accessed on August 2019.
2. https.//pubchem.ncbi.nlm.nih.gov/compound/9871420, accessed on August 2019.
3. Beckett A H. and Stenlake J B. Practiacal Pharmaceutical Chemistry. part-II. 4th Edn. CBS publishers and distributors. India. 1997.pp 281.
4. Davidson A G. Ultra-visible absorption Spectrophotometry. Practical Pharmaceutical chemistry. 4th Edn, part-II. CBS publisher and distributors. 1997. Pp 275-337
5. ICH Guideline Q2B, Validation of Analytical Procedures, Methodology. 1996.1.
6. N. Patel. D. et al A Review on Chromatographic And Spectrophotometric Methods For Estimation Of Gliflozin In Bulk And In Different Dosage Forms. Journal of Global Trends In Pharmaceutical Sciences, JGTPS, 2015; Vol. 6(4):2920 – 2924
7. I. muzaffar et al A simple and sensitive high performance liquid chromatography assay with a fluorescence detector for determination of canagliflozin in human plasma. Anal. Methods. 2015;3028-3035. Accessed on 13th nonmember 2016. doi:10.1039/C5AY00074B
8. Ishpreet kaur et al Determination of canagliflozin in bulk and pharmaceutical Dosage form. Pharm methods, 2015; 6(2): 82-86. doi:10.5530/phm.2015.6.11
9. Deepak gaware. R. N. Patil and Mangesh harole. A validated stability indicating rp-HPLC method for simultanious determination of metformin and canagliflozin in pharmaceutical formulation.World journal of pharmacy and pharmaceutical sciences. 4(12): 631-640. doi:10.13040/IJPSR.0975-8232.8(8).3427-35
10. Uttam prasad panigrahy et al A novel validated rp-HPLC-dad method for the Simultaneous estimation of metformin hydrochloride and canagliflozin in bulk and pharmaceutical tablet Dosage form with forced degradation studies. Oriental journal of chemistry.2015; vol. 31(3):1489-1507.doi: 10.13005/ojc/310328
11. Maddu Suma et al RP-HPLC method development and validation for the estimation of canagliflozin in tablet dosage form .Int j pharm 2014;5(4): 1288-1292.
12. Mahawar Sheetal. A Simple Ultraviolet Spectrophotometric Method for the Estimation of Docetexel in Bulk Drug and Formulation. Asian Journal of Pharmaceutical Analysis.3 (2): 2013;48-52.
13. Patel Juhee J. Henal Chorawala, Zana R. Dedania, S.M. Vijendraswamy. Development and Validation of UV Spectroscopic Method for Simultaneous Estimation of Doxofylline and Terbutaline Sulphate in Combined Dosage Form. Asian Journal of Pharmaceutical Analysis.5 (2): 2015;74-78.
14. Suman Pattanayak et al Analytical UV Spectroscopic Method Development and Validation for the Estimation of Mycophenolate Mofetil. Asian Journal of Pharmaceutical Analysis. 5(4):2015;209-213.
15. Dave Vidhi. Paresh Patel. Method development and Validation of UV Spectrophotometric estimation of Remogliflozin Etabonate in bulk and its tablet dosage form. Research Journal of Pharmacy and Technology. 2021.14(4):2042-4.doi: 10.52711/0974-360X.2021.00362
16. Shubhangi C. Daswadkar et al Determination of Zolmitriptan in Solid Dosage Form by UV Spectroscopic Method Asian Journal of Pharmacy and Technology.2019;9(4):244-248. doi:10.5958/2231-5713.2019.00040.0
17. N. Jagruthi. Shalem K et al Development and Validation of UV Specroscopic Method for the estimation of Sofosbuvir in and Pharmaceutical Dosage Form. Asian Journal of Research in Chemistry.2018;11(3):563-568. doi: 10.5958/2231-5675.2020.00035.6
18. Dipti G. Phadtare et al Validated UV Spectroscopic Method for Estimation of Montelukast Sodium from Bulk and Tablet Formulations. Asian Journal of Research in Chemistry. 2016;9(7).335-338
19. Pankaj Savan et al A Review. Determination of Clopidogrel Bisulphate in Biological Fluid and Pharmaceutical Dosage Forms. Research Journal of Pharmaceutical Dosage Form and Technology. 8(1). 2016; 66-70.
20. Kumaraswamy G et al Spectrophotometric Determination of Tenatoprazole in Bulk Drug and Pharmaceutical Dosage Form. Research J. Pharm. and Tech. 4(4). April 2011: 574-577.
21. Prafulla M Sabale et al Simple Spectrophotometric Methods for the Determination of Zn-Sparfloxacin 1, 10 – Phenanthroline Mofetal complex. Research Journal of pharmacy and Technology.2013 ;6(6): 614-617.
22. Garlapati Mandeep et al Development and Validation of a UV Spectroscopic Method to Estimate Etoricoxib in Bulk and Tablet Formulation. Research Journal of pharmacy and Technology.2018;11(2):758-760.
23. DVS Roopa Sirisha et al Development and Validation of a UV Spectroscopic Method for the Determination of Ranolazine in Bulk and Formulation. Research Journal of pharmacy and Technology.2019;12(10):5007-5010.doi: 10.46624
24. D. Sridharan, Umarani et al Development and Validation of UV Spectrophotometric Method of Darifenacin Hydrobromide in Bulk and Tablet Dosage Form. Asian J. Pharm. Ana.2011; 1(11).43-45.
25. Asma Afroz, et al Spectrophotometric Estimation of Rosuvastatin Calcium and Glimepiride in Tablet Dosage Form. Asian J. Pharm. Ana. 2011; 1(4):74-78.
26. Ghanshyam B et al Spectrophotometric Determination and Validation of Zidovudine Concentration in Bulk and Dosage form. Asian J. Pharm. Ana. 2014;4(2): 51-53.doi: 10.2478/v10007-008-0017-0
27. Khandelwal Pankaj et al Absorbances Ratio Method (Isoabsorptive Point) for Determination of Drotaverine Hydrochloride and Ranitidine Hydrochloride from Pharmaceutical Dosage Forms. Asian J. Pharm. Ana. 2014; 4(3):113-115.
28. Manoranjan Sabat et al A New Analytical Method Development and Validation for Estimation of Ciprofloxacin in Bulk and Pharmaceutical Dosage Form. Asian J. Pharm. Ana. 2012; 2(4): 116-117.