Author(s): Amit Chaudhary, Bhuvnesh Kumar Singh

Email(s): bhuvneshiftm@gmail.com

DOI: 10.52711/0974-360X.2022.00791   

Address: Amit Chaudhary1, Bhuvnesh Kumar Singh1,2*
1School of Pharmacy, Abhilashi University, Mandi, Himachal Pradesh, India.
2Teerthanker Mahaveer University, Moradabad, Uttar Pradesh, India.
*Corresponding Author

Published In:   Volume - 15,      Issue - 10,     Year - 2022


ABSTRACT:
In this study, a novel technique was developed and validated for the simultaneous estimation of Remogliflozin (REMO) and Metformin (MET) in bulk and formulation. The separation was achieved using ODS, C18 (250mm × 4.6mm, i.d.2.5µm) column with isocratic elution. A mixture of acetonitrile: 20 mM ammonium formate buffer (pH 3.5) at the ratio of (60:40) as mobile phase with a flow rate of 1.0ml min-1 was used for the separation of analytes. The separated effluents were monitored with diode array detector at 243nm. The results showed good linearity over a range of 2.5–25µg ml-1 and 12.5–125µg ml-1 for REMO and MET respectively. Furthermore, the average percentage assay of marketed formulations of REMO and MET was found to be 100.30% and 100.52%, respectively. REMO limit of detection (LOD) and limit of quantitation (LOQ) were 0.42 and 1.28µg ml-1 while LOD and LOQ for MET were 1.97 and 5.96µg ml-1 respectively. The proposed method was simple, rapid and precise for the study of new formulations which has been approved for the cure of diabetes mellitus and applicable for the analysis of both drugs in pharmaceutical industries effectively.


Cite this article:
Amit Chaudhary, Bhuvnesh Kumar Singh. Method Development and Validation for simultaneous Quantification of Remogliflozin and Metformin in Bulk and Tablets by RP-HPLC. Research Journal of Pharmacy and Technology 2022; 15(10):4709-4. doi: 10.52711/0974-360X.2022.00791

Cite(Electronic):
Amit Chaudhary, Bhuvnesh Kumar Singh. Method Development and Validation for simultaneous Quantification of Remogliflozin and Metformin in Bulk and Tablets by RP-HPLC. Research Journal of Pharmacy and Technology 2022; 15(10):4709-4. doi: 10.52711/0974-360X.2022.00791   Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2022-15-10-64


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