ABSTRACT:
The study was carried out to prepare, characterize, and investigates the pharmacological evaluation of Bauhinia variegata lauha bhasma (BVLB). The Lauha bhasma (LB) was prepared according to the reported standard method that involved the destruction of metallic state followed by conversion of crude product into bhasma state. The purification was done using Triphala extract prepared in cow-urine. The destruction of the metallic test was carried out using process juice of BV. At the last, the preparation of BVLB is subjected to a closed crucible system to get the final product. The characterization of BVLB was done by some preliminary examination followed by specific methods viz. X-ray diffractometry (XRD), Infrared (IR) spectroscopy, and UV-visible spectrophotometry. The In-vitro anti-inflammatory, anti-arthritic and antioxidant activity was carried out for BVLB at different concentrations of 50, 100, 200, and 250µg/mL. The said In-vitro activities were screened using percent inhibition of heat-induced hemolysis, the percent inhibition of protein denaturation, proteinase inhibition, and DPPH free radical scavenging activity. BVLB preliminary investigation showed 1.53 % aqueous and 3.95 % HCL solubility whereas specific characterization methods showed the formation of micro and nanocrystals of F2CO3. The BVLB showed % inhibition of hemolysis of in between 5.26 ± 0.95 to 31.20±4.12 whereas percent inhibition of protein denaturation in between 12 ± 1.22 to 52.22 ± 4.69. The percent proteinase inhibition of BVLB at a different concentration from 50, 100, 200, and 250µg/mL was estimated and found in between 3.26 ± 0.86 to 51.22 ± 3.12. The free radical scavenging activity of BVLB was found from 10.11 1 ± 0.89 to 52.05 ± 2.05. From the current investigation, it can be conducted that BVLB has significant In-vitro anti-inflammatory, anti-arthritic and antioxidant activity. The formed nanocrystals and presence of BV active constituents would be the underlying intermediaries for significant activity.