Author(s):
Gajender Singh, Dheeraj Bisht, Rajeshwar Kamal Kant Arya, Sunil Kumar, Deepak Kumar, Ashutosh K. Dash
Email(s):
ashutosh.dash10@gmail.com , guptadeepak002@gmail.com
DOI:
10.52711/0974-360X.2022.00974 
Address:
Gajender Singh1, Dheeraj Bisht2, Rajeshwar Kamal Kant Arya2, Sunil Kumar1, Deepak Kumar1*, Ashutosh K. Dash3*
1Department of Pharmaceutical Chemistry, School of Pharmaceutical Sciences, Shoolini University, Solan - 173229, India.
2Department of Pharmaceutical Sciences, Sir J. C. Bose Technical Campus Bhimtal, Kumaun University, Nainital - 263136, Uttarakhand, India.
3Devsynthesis India Pvt. Ltd, Drug Discovery Chemistry CRO, Hyderabad, India – 500076.
*Corresponding Author
Published In:
Volume - 15,
Issue - 12,
Year - 2022
ABSTRACT:
Picrorhiza kurroa (kutki) is a hairy herb belonging to Plantaginaceae/ Scropulariacea family and found in some parts of India (Kashmir to Sikkim), Nepal, and China.They found application in various ailments such as inflammation, antioxidant activity, asthmatic activity, hepatic activity, etc. The compound constituents using HPLC retrieved the presence of eighteen compounds. HPTLC analysis confirmed and quantified the presence of six major compounds. The quantity of diosgenin was found to be (2.80%) maximum followed by chlorogenic acid (2.70% w/w of extract) in the DCM/Methanol leaf extract. Antibacterial activity was performed using the zone of inhibition method for eight different leaf extracts of Picrorhiza kurroa. Among the extracts, ethyl acetate extract showed good potency in terms of zone of inhibition (13.33±1.52) against S. aureus whereas n-butanol extract showed good potency (12.01±1.00) against S. mutans, acetone extracts showed good potency (16.66±.47) against E. coli, acetone extracts showed good potency (11.33±1.52) against K. pneumoniae and acetone extracts showed good potency (11.00±1.00) against P. aeruginosa. Methanol extract showed good antioxidant activity (41.3±3.1µg/ml) whereas n-hexane showed the least (389.5±1.3µg/ml). Chlormphenicol and Ascorbic acid were taken as standard in the antibacterial and antioxidant study. IC50 values were calculated from the known protocol.
Cite this article:
Gajender Singh, Dheeraj Bisht, Rajeshwar Kamal Kant Arya, Sunil Kumar, Deepak Kumar, Ashutosh K. Dash. Identification and Quantification of six natural compounds from Picrorhiza kurroa leaf extract and their Antibacterial and Antioxidant activity. Research Journal of Pharmacy and Technology 2022; 15(12):5774-8. doi: 10.52711/0974-360X.2022.00974
Cite(Electronic):
Gajender Singh, Dheeraj Bisht, Rajeshwar Kamal Kant Arya, Sunil Kumar, Deepak Kumar, Ashutosh K. Dash. Identification and Quantification of six natural compounds from Picrorhiza kurroa leaf extract and their Antibacterial and Antioxidant activity. Research Journal of Pharmacy and Technology 2022; 15(12):5774-8. doi: 10.52711/0974-360X.2022.00974 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2022-15-12-68
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