Author(s): Jai Bharti Sharma, Shailendra Bhatt, Vipin Saini, Rupesh K. Gautam, Manish Kumar

Email(s): shailu.bhatt@gmail.com

DOI: 10.52711/0974-360X.2022.00107   

Address: Jai Bharti Sharma1, Shailendra Bhatt1*, Vipin Saini2 , Rupesh K. Gautam3, Manish Kumar1
1MM College of Pharmacy, MM (Deemed to be) University, Mullana, Ambala, Haryana, India.
2Maharishi Markandeshwar University, Solan, HP, India.
3MM School of Pharmacy, MM University, Sadopur, Ambala, Haryana, India.
*Corresponding Author

Published In:   Volume - 15,      Issue - 2,     Year - 2022


ABSTRACT:
Background: Curcumin shows degradation in the solution of high pH. There is a need for development of a method which can estimate the drug release precisely and accurately. The UV spectroscopy due to its simplicity and specificity is the most popular for method development for the determination of drugs. Objective: The present study was aimed to develop the UV-Visible spectrophotometric method for the assessment of curcumin and tetrahydrocurcumin (THC) using a stable solvent system. Method: The solubility of curcumin and tetrahydrocurcumin was determined at various physiological pH levels. Non-ionic surfactant, tween 80 was used with selected media to avoid the degradation of drugs. The selected solvent system was further validated according to guidelines of the international conference on harmonization (ICH) and the analytical parameters like linearity, veracity, sensitivity and accuracy were studied. Results: The solvent systems Phosphate buffer of pH 6.8+2% tween 80 and Phosphate buffer of pH 6.8+1% tween 80 were found optimum for estimation of curcumin and THC respectively. All the validation parameters were found within the range for developed methods. Conclusion: The proposed method is very simple and can be used for routine quality control testing of curcumin and tetrahydrocurcumin.


Cite this article:
Jai Bharti Sharma, Shailendra Bhatt, Vipin Saini, Rupesh K. Gautam, Manish Kumar. UV-Visible Spectrophotometric Method Development and Validation for the Estimation of Curcumin and Tetrahydrocurcumin. Research Journal of Pharmacy and Technology. 2022; 15(2):650-4. doi: 10.52711/0974-360X.2022.00107

Cite(Electronic):
Jai Bharti Sharma, Shailendra Bhatt, Vipin Saini, Rupesh K. Gautam, Manish Kumar. UV-Visible Spectrophotometric Method Development and Validation for the Estimation of Curcumin and Tetrahydrocurcumin. Research Journal of Pharmacy and Technology. 2022; 15(2):650-4. doi: 10.52711/0974-360X.2022.00107   Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2022-15-2-27


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