Author(s): Rajamehala M, Renugaa Su, Muthu Kumara Pandian A, Gopalakrishnan B, Vijay Pradhap Singh M


DOI: 10.52711/0974-360X.2022.00115   

Address: Rajamehala M1*, Renugaa Su1, Muthu Kumara Pandian A1, Gopalakrishnan B2 , Vijay Pradhap Singh M1
1Department of Biotechnology, Vivekanandha College of Engineering for Women, Thiruchengode, Tamil Nadu, India.
2Department of Chemical Engineering, Annamalai University, Chidambaram, Tamil Nadu, India.
*Corresponding Author

Published In:   Volume - 15,      Issue - 2,     Year - 2022

In recent years, nanotechnology was an emerging field which has wide range of application in various science fields. Nanoparticles has significant approaches in medicinal field. The nanoparticles can be synthesized from various sources depending upon the applications. In this current study, the green synthesis of Titanium dioxide nanoparticles from Calotropis gigantae and its antifungal activity was studied. The precursor for titanium nanoparticles is titanium tetraisopropoxide. Usually the plant material assisted in reduction process. The green synthesis approach was eco-friendly, cost effective, non-toxic and renewable resources. Titanium dioxide nanoparticles were characterized by SEM, FTIR, XRD and EDAX. Scanning Electron Microscope analysis gives the details of TiO2 nanoparticles spherical shape and other structural properties. X-Ray Diffraction analysis gives the crystalline nature of Titanium dioxide Nanoparticles. Fourier Transform Infrared spectroscopy results indicates the presence of various bioactive compounds. Energy Dispersive X-ray Analysis brings about the elemental composition of titanium nanoparticles. Titanium dioxide nanoparticles shows the antifungal activity against particular microorganism.

Cite this article:
Rajamehala M, Renugaa Su, Muthu Kumara Pandian A, Gopalakrishnan B, Vijay Pradhap Singh M. Green Synthesis of Titanium Dioxide and its Application on Anti-Fungal Activity. Research Journal of Pharmacy and Technology. 2022; 15(2):695-0. doi: 10.52711/0974-360X.2022.00115

Rajamehala M, Renugaa Su, Muthu Kumara Pandian A, Gopalakrishnan B, Vijay Pradhap Singh M. Green Synthesis of Titanium Dioxide and its Application on Anti-Fungal Activity. Research Journal of Pharmacy and Technology. 2022; 15(2):695-0. doi: 10.52711/0974-360X.2022.00115   Available on:

1.    Happy Agarwal, S Venkat Kumar, and S Rajesh Kumar. A review on green synthesis of Zinc Oxide nanoparticles – An eco-friendly approach. Resource Efficient Technologies. 2017; 4(3): 406-413.
2.    Soumya Menon, Rajesh Kumar S, Venkat Kumar S. A review on biogenic synthesis of gold nanoparticles; characterization and its applications. Resource Efficient Technologies. 2017; 4(3): 516- 527.
3.    Rajesh Kumar S and Poonam Naik. Synthesis and biomedical applications of Cerium oxide nanoparticles – A Review. Biotechnology Reports. 2017; 17: 1-5.
4.    Kubacka A, Diez MS, Rojo D, Rafael Bargiela R, Ciordia S, Zapico I et al. Understanding the antimicrobial mechanism of TiO2-based nanocomposite films in a pathogenic bacterium. Nat Sci Rep. 2014; 4 (4134).
5.    Ijadpanah-Saravi H, Safari M, Khodadadi-Darban A, Rezaei A. Synthesis of titanium dioxide nanoparticles for photocatalytic degradation of cyanide in waste water. Anal Lett.  2014; 47: 1772–1782
6.    Karthick Raja Namasivayam1 S, Jayakumar D, Ramesh Kumar V, Arvind Bharani R.S. Anti-Bacterial and Anti Cancerous Biocompatible Silver Nanoparticles Synthesized from the Cold Tolerant Strain of Spirulina platensis. Research J. Pharm. and Tech. 2014; 7(12).
7.    Sukanya SL, Sudisha J, Hariprasad P, Niranjana SR, Prakash HS, Fathima SK. Antimicrobial activity of leaf extracts of Indian medicinal plants against clinical and phytopathogenic bacteria. Afr J Biotechnol. 2009; 8 (23).
8.    Aditya Jain, S Rajeshkumar, Anitha Roy. Anti-inflammatory activity of Silver nanoparticles synthesized using Cumin oil. Research J. Pharm. and Tech. 2019; 12(6).
9.    Ambikapathy V, Gomathi S, Paneerselvam A. Effect of antifungal activity of some medicinal plants against Pythium debaryanum (Hesse). Asian J Plant Sci Res. 2011; 1(3): 131-134.
10.    Amalraj T, Ignacimuthu S. Hyperglycemic effect of leaves of Mimosa pudica L. Fitotherapia. 2002; 73(4): 351-2.
11.    Gamble J S; Flora of the Presidency of Madras, Vol. I, II, III, Botanical survey of India, Calcutta, 1935.
12.    Murti PBR and Seshadri TR; Chemical composition of Calotropis gigantae: Part1. Wax and Resin components of the latex, Proc. Ind. Acad. Sci.1943; 18: 145 – 159.  
13.    Mukunthan K, Balaji S. Cashew apple juice (Anacardium occidental L) speeds up the synthesis of silver nanoparticles. Int. J. Green Nanotechnol. 2012; 4: 71-79
14.    Subhapriya, S.; Gomathipriya, P. Green Synthesis of Titanium Dioxide (TiO2) Nanoparticles by Trigonella foenum-graecum Extract and Its Antimicrobial Properties. Microb. Pathogen. 2018;116: 215–220.
15.    V. Kumar, Yadav S.K. Plant mediated synthesis of silver and gold nanoparticles and their applications. J. Chem. Technol. Biotechnol. 2009; 84:151-7.
16.    Cho EJ, Holback H, Liu KC, Abouelmagd SA, Park J, Yeo Y. Nanoparticle characterization: state of the art, challenges, and emerging technologies. Mol Pharm. 2013; 10: 2093–110.  
17.    Santhoshkumar, T., Rahuman, A.A., Jayaseelan, C., Rajakumar, G., Marimuthu, S., Kirthi, A.V., Velayutham, K., Thomas, J., Venkatesan, J., Kim, S.K. Green synthesis of titanium dioxide nanoparticles using Psidium guajava extract and its antibacterial and antioxidant properties. Asian Pac. J. Trop. Med. 2014; 7: 968–976.
18.    Ambika, S.; Sundrarajan, M. [EMIM] BF4 Ionic Liquid-Mediated Synthesis of TiO2 Nanoparticles Using Vitex negundo Linn Extract and Its Antibacterial Activity. J. Mol. Liq. 2016; 221: 986–992.
19.    Vasconcelos, D.C., Costa, V.C., Nunes, E.H., Sabioni, A.C., Gasparon, M., Vasconcelos, W.L. Infrared spectroscopy of titania sol-gel coatings on 316L stainless steel. Mater. Sci. Appl. 2011; 2: 1375–1382.
20.    Yilmaz,M., Turkdemir, H., Kilic,M.A., Bayram, E., Cicek, A.,Mete, A., Ulug, B. Biosynthesis of silver nanoparticles using leaves of Stevia rebaudiana. Mater. Chem. Phys. 2011; 130: 1195–1202.
21.    Tuoriniemi, J., Johnsson, A.C., Holmberg, J.P., Gustafsson, S., Gallego-Urrea, J.A., Olsson, E., Pettersson, J.B., Hassellov, M. Intermethod comparison of the particle size distributions of colloidal silica nanoparticles. Sci. Technol. Adv. Mater. 2015; 15: 1–10.
22.    Rajakumar. G, Abdul Rahuman A, Priyamvada B, Gopiesh Khanna V, Kishore Kumar D, Suzin PJ. Eclipta prostrata leaf aqueous extract mediated synthesis of titanium dioxide nanoparticles. Materials Let. 2012; 68:115-7
23.    Tabir M.B et al., Morphology tailored synthesis of C-WO3 Nano structures and its photocatalytic application. J. Inorg. Organomet. Polym. Mater. 2018; 28(3), 738-745.
24.    Patterson, A. The Scherrer formula for X-ray particle size determination. Phys. Rev. 1939; 56: 978–982.
25.    Santhoshkumar, T.; Rahuman, A. A.; Kirthi, A. V.; Kim, S. K. Green Synthesis of Titanium Dioxide Nanoparticles Using Psidium guajava Extract. Asian Pac. J. Trop. Med. 2013; 5: 245–256.
26.    Ghulam Nabi, Waseem Raza, Tabir M.B. Green synthesis of TiO2 Nanoparticles using Cinnamon powder extract and the study of Optical properties. Journal of Inorganic and Organometallic Polymers and Materials. 2019; 7.
27.    Nasrollahzadeh M, Atarod M, Jaleh B, Gandomirouzbahani M. In situ green synthesis of Ag nanoparticles on graphene oxide/TiO2 nanocomposite and their catalytic activity for the reduction of 4-nitrophenol, Congo red andmethylene blue. Ceram. Int. 2016; 42: 8587–8596.
28.    Lai Y, Wang L, Liu D, Chen Z, Lin C. TiO2-based nanomaterials: design, synthesis and applications. J Nanomaterials. 2015; 1–3.
29.    Gazquez M, Bolívar J, Garcia-Tenorio R, Vaca F. A Review of the production cycle of titanium dioxide pigment. Mater Sci App. 2014; l 5:441–458.
30.    Jovanovic B. Critical Review of Public Health Regulations of titanium dioxide, a human food additive. Integ Environ Assess Manag. 2014; 11:10–20.
31.    Goffredo G, Munafo P. Preservation of historical stone surfaces by TiO2 Nano coatings. Coatings. 2015; 5:222–231.
32.    Ijadpanah-Saravy H, Dehestaniathar Khodadadi A, Safari M. Optimization of photocatalytic degradation of β-naphthol using nano TiO2- activated carbon composite. Desalin Water Treat. 2016; 57:4708–4719.
33.    Ijadpanah-Saravi H, Sarafi M, Noruzi-Masir B, Darban AK, Bakhshi P. Intelligent tools to model photo-catalytic degradation of titanium dioxide nanoparticles. J Chemom. 2017; 31:e2907–e2909.
34.    Inagaki I, Takechi T, Shirai Y, Ariyasu N. Application and features of titanium for aerospace industry. Nippon Steel Sumitomo. Met Tech Rep. 2013; 396:23–28.
35.    Wiesenthal A, Hunter L, Wang S, Wickliffe J, Wilkerson M. Nanoparticles: small and mighty. Int J Dermatol. 2011; 50:247–254.
36.    Yuan Y, Ding J, Xu J, Deng J, Guo J. TiO2 nanoparticles codoped with silver and nitrogen for antibacterial application. J Nanosci Nanotechnol. 2010; 10:4868–4874.
37.    Szacilowski K, Macyk W, Drzewiecka -Matuszek A, Brindell M, Stochel G (2005) Bioinorganic photochemistry: frontiers and mechanisms. Chem Rev. 2005; 105:2647–2694.
38.    Rai R.V, Bai J.A. Nanoparticles and their potential application as antimicrobials. Science against microbial pathogens: communicating current research and technological advances. Microbiology Series 3. Formatex, Spain. 2011; 197-209.
39.    M. Gericke, A. Pinches, Biological synthesis of metal nanoparticles, Hydrometallurgy. 2006; 83,132–140.
40.    Carneiro C, Casbane E. Diterpene as a Promising Natural Antimicrobial Agent against Biofilm associated Infection. Molecules. 2011; 16: 190-1.
41.    Donelli G, Francolini I, Romoli D, Guaglianone E, Piozzi A, Ragunath C, et al. Synergistic activity of dispersin B and cefamandolenafate in inhibition of staphylococcal biofilm growth on polyurethanes. Antimicrob Agents Chemother. 2007; 51: 2733–40.

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