Baru Sadarun, Wahyuni, Muhammad Hajrul Malaka, Adryan Fristiohady, Agung Wibawa Mahatma Yodha, Nur Syifa Rahmatika, Zulfikri Saleh Islami, Muhammad Nurjayadin, Carla Wulandari Sabandar, Ahmad Darmawan, Andini Sundowo, Andi Rifky Rosandi, Sahidin I
Baru Sadarun1, Wahyuni2, Muhammad Hajrul Malaka2, Adryan Fristiohady2, Agung Wibawa Mahatma Yodha2, Nur Syifa Rahmatika3, Zulfikri Saleh Islami3, Muhammad Nurjayadin3, Carla Wulandari Sabandar4, Ahmad Darmawan5, Andini Sundowo5, Andi Rifky Rosandi6, Sahidin I2*
1Faculty of Fisheries and Marine Sciences, Universitas Halu Oleo, Kendari 93232, Indonesia.
2Faculty of Pharmacy, Universitas Halu Oleo, Kendari 93232, Indonesia.
3Faculty of Medicine, Universitas Halu Oleo, Kendari 93232, Indonesia.
4Department of Pharmacy, Faculty of Science and Technology, Universitas Sembilanbelas November Kolaka, Kolaka, Indonesia.
5Research Centre for Chemistry, National Research and Inovation Agency (BRIN), Kawasan Puspitek, Tangerang Selatan, Banten, Indonesia.
6School of Chemical Sciences and Food Technology, Universiti Kebangsaaan, Malaysia, 46000 Bangi, Selangor, Malaysia.
Volume - 15,
Issue - 4,
Year - 2022
Xestospongia sp. is one of marine sponge that can be found in Southeast Sulawesi. It belongs to Demospongiae classes which have shown many pharmacological activities such as antioxidant. Thus, this study aimed to identify isolates from Xestospongia sp. and its activity as antioxidant and anti-inflammatory. Isolation were carried out by chromatography technique including Thin Layer Chromatrography (TLC), vacuum liquid chromatography (VLC) and radial chromatography (RC) with silica gel as an adsorbent. Structure of isolated compounds were determined by spectroscopy methods i.e. FTIR, 1H and 13C NMR and also by comparison with those reported values. Biological activity of Xestospongia sp was also evaluated using DPPH (2,2-diphenyl-1-picrylhydrazyl) radicals and Human Red Blood Cell (HRBC) methods. Four compounds isolated and identified from methanol extract of Xestospongia sp. were steroids that are (1) purchrasterol, (2) xestosterol, (3) saringosterol, and (4) 5a,8a-epidioxy-24a-ethylcholest-6-en-3ß-ol. The extract and the compounds showed antioxidant activity against DPPH radicals in which the extract was stronger than the isolated compounds. Furthermore, the Xestospongia sp. extract exhibited a dose-dependant anti-inflammatory activity by stabilizing red blood cell membranes at concentrations ranging from 50 to 3200 ppm. In conclusion, Xestospongia sp. extract which contain sterol compounds, such as purchrasterol, xestosterol, saringosterol and 5a,8a-epidioxy-24a-ethylcholest-6-en-3ß-ol provides antioxidant and anti-inflammatory activity.
Cite this article:
Baru Sadarun, Wahyuni, Muhammad Hajrul Malaka, Adryan Fristiohady, Agung Wibawa Mahatma Yodha, Nur Syifa Rahmatika, Zulfikri Saleh Islami, Muhammad Nurjayadin, Carla Wulandari Sabandar, Ahmad Darmawan, Andini Sundowo, Andi Rifky Rosandi, Sahidin I. Biological activities of Steroids and Extracts from Xestospongia sp. growing in Southeast Sulawesi (Indonesia). Research Journal of Pharmacy and Technology. 2022; 15(4):1487-3. doi: 10.52711/0974-360X.2022.00247
Baru Sadarun, Wahyuni, Muhammad Hajrul Malaka, Adryan Fristiohady, Agung Wibawa Mahatma Yodha, Nur Syifa Rahmatika, Zulfikri Saleh Islami, Muhammad Nurjayadin, Carla Wulandari Sabandar, Ahmad Darmawan, Andini Sundowo, Andi Rifky Rosandi, Sahidin I. Biological activities of Steroids and Extracts from Xestospongia sp. growing in Southeast Sulawesi (Indonesia). Research Journal of Pharmacy and Technology. 2022; 15(4):1487-3. doi: 10.52711/0974-360X.2022.00247 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2022-15-4-13
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