Nelly Zuroidah, May Fanny Tanzilia, I Gusti Agung Ayu Eka Putri Sunari, Billy Jordan Wrahatnala, Faradila Khoirun Nisa Haki, Aryati, Puspa Wardhani, Dominicus Husada, Ali Rohman, Siti Nadia Tarmizi
Nelly Zuroidah1, May Fanny Tanzilia2, I Gusti Agung Ayu Eka Putri Sunari1, Billy Jordan Wrahatnala3, Faradila Khoirun Nisa Haki3, Aryati4,5*, Puspa Wardhani4,5, Dominicus Husada6, Ali Rohman7, Siti Nadia Tarmizi8
1Clinical Pathology Specialization Program, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia.
2Clinical Pathology Sub-Specialization Program, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia.
3Master Program of Tropical Medicine, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia.
4Department of Clinical Pathology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia.
5Institute of Tropical Disease, Universitas Airlangga, Surabaya, Indonesia.
6Department of Pediatric, Faculty of Medicine – Universitas Airlangga, Surabaya.
7Department of Chemistry, Faculty of Science and Technology – Universitas Airlangga, Surabaya.
8Directorate of Vector Borne and Zoonotic Disease, Ministry of Health Republic of Indonesia, J
Volume - 15,
Issue - 4,
Year - 2022
Background : NS1 is currently widely used for diagnosis of dengue virus (DENV) infection. Various methods are used to diagnose DENV infection (DVI), either ELISA, immunochromatography (ICT) or most recently the fluorescence immunoassay (FIA) method which are commercially available. Objective: This study aimed to compare the detection capabilities of dengue NS1 antigens using (1) Dengue NS1 ICT Ag (Standard Q - SD Biosensor, Inc.), (2) Dengue NS1 ICT Ag (SD Bioline - Standard Diagnostic, Inc), and (3) Dengue NS1 Ag FIA (Standard F - SD Biosensor, Inc.) Methods: This study consisted of serum samples (n=80) with the number of DVI patients (n=50), non-DVI (n=30). All samples were examined using all three commercial kits for NS1 antigen testing. All DVI samples showed results of reverse-transcriptase polymerase chain reaction (RT-PCR - SIMPLEXA?? Dengue - Focus Diagnostics) and/or positive dengue NS1 (Panbio® Dengue Early ELISA) antigen. Results: Standard F showed the highest sensitivity (82%) compared to Standard Q (74%) and SD Bio line (74%). These three commercial kits had the same specificity 100%. The positive predictive value all of these kits was 100% each. The negative prediction value of Standard F, Standard Q, and SD Bio line were 76.9%, 63.8%, 63.8%, respectively. These three NS1 antigen tests had a good agreement (? 0.681-0.774). Conclusions: FIA test performance (Standard F SD - Biosensor, Inc.) were a quick and easy examination, showing a higher sensitivity and specificity than ICT for detecting DENV infection. Further research is needed to confirm the diagnosis of primary or secondary infection.
Cite this article:
Nelly Zuroidah, May Fanny Tanzilia, I Gusti Agung Ayu Eka Putri Sunari, Billy Jordan Wrahatnala, Faradila Khoirun Nisa Haki, Aryati, Puspa Wardhani, Dominicus Husada, Ali Rohman, Siti Nadia Tarmizi. Comparison of Diagnostic Tests for Detection of Nonstructural-1(NS1) Antigen Dengue virus using Immunochromatography and Fluorescence Immunoassay Methods. Research Journal of Pharmacy and Technology. 2022; 15(4):1494-8. doi: 10.52711/0974-360X.2022.00248
Nelly Zuroidah, May Fanny Tanzilia, I Gusti Agung Ayu Eka Putri Sunari, Billy Jordan Wrahatnala, Faradila Khoirun Nisa Haki, Aryati, Puspa Wardhani, Dominicus Husada, Ali Rohman, Siti Nadia Tarmizi. Comparison of Diagnostic Tests for Detection of Nonstructural-1(NS1) Antigen Dengue virus using Immunochromatography and Fluorescence Immunoassay Methods. Research Journal of Pharmacy and Technology. 2022; 15(4):1494-8. doi: 10.52711/0974-360X.2022.00248 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2022-15-4-14
1. Aryati A et al. Dengue Virus Serotype 4 Is Responsible for the Outbreak of Dengue in East Java City of Jember, Indonesia. Viruses. 2020; 12(9).
2. Budiarti R et al. Comparative analysis of qpcr measurement of hiv viral load and elisa detection of p24 antigen after hyperbaric oxygen exposure. African Journal of Infectious Diseases. 2020; 14(2):53–9.
3. Aryati A et al. Performance of commercial dengue NS1 ELISA and molecular analysis of NS1 gene of dengue viruses obtained during surveillance in Indonesia. BMC infectious diseases. 2013; 13(1):611.
4. Salawati L, Siregar M, Hasibuan RS. Hubungan pemberantasan sarang nyamuk dengan keberadaan jentik di Kecamatan Jaya Baru Banda Aceh Tahun 2017. Medicus Darussalam. 2018; 1(1):18–24.
5. Wardhani P et al. Clinical and virological characteristics of dengue in Surabaya, Indonesia. PLoS One. 2017; 12(6):e0178443.
6. Lee H et al. Comparison of Six Commercial Diagnostic Tests for the Detection of Dengue Virus Non-Structural-1 Antigen and IgM/IgG Antibodies. Annals of laboratory medicine. 2019; 39(6):566–71.
7. Zammarchi L et al. Evaluation of a new rapid fluorescence immunoassay for the diagnosis of dengue and Zika virus infection. Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology. 2019 Mar; 112:34–9.
8. Lee H et al. Comparison of six commercial diagnostic tests for the detection of dengue virus non-structural-1 antigen and IgM/IgG antibodies. Annals of laboratory medicine. 2019; 39(6):566–71.
9. Organization WH et al. Dengue: guidelines for diagnosis, treatment, prevention and control. World Health Organization; 2009.
10. Kandhakumari G, Stephen S. Extra pulmonary tuberculosis: Rapid identification of Mycobacterium tuberculosis grown in Mycobacterium growth indicator tube 960 and Lowenstein-Jensen media, employing Standard diagnostics Bioline Mycobacterium tuberculosis protein 64 antigen detection k. Indian journal of medical microbiology. 2015; 33(5):122.
11. Shan X et al. Evaluation of the diagnostic accuracy of nonstructural protein 1 Ag-based tests for dengue virus in Asian population: a meta-analysis. BMC infectious diseases. 2015; 15(1):360.
12. Darwish NT et al. Immunofluorescence–based biosensor for the determination of dengue virus NS1 in clinical samples. Journal of Pharmaceutical and Biomedical Analysis. 2018; 149:591–602.
13. Xia X et al. A highly sensitive europium nanoparticle-based lateral flow immunoassay for detection of chloramphenicol residue. Analytical and bioanalytical chemistry. 2013; 405(23):7541–4.
14. STANDARD Q. Rota/Adeno Ag.
15. Akualing JS et al. Identification Of Dengue Virus Serotypes At The Dr. Soetomo Hospital Surabaya In 2016 And Its Correlation With Ns1 Antigen Detection. Indonesian Journal of Clinical Pathology and Medical Laboratory. 2017;23(2):151–6.
16. Bishop JD et al. Sensitivity enhancement in lateral flow assays: A systems perspective. Lab on a Chip. 2019; 19(15):2486–99.
17. Vongsouvath M et al. Using rapid diagnostic tests as a source of viral RNA for dengue serotyping by RT-PCR-a novel epidemiological tool. PLoS neglected tropical diseases. 2016; 10(5):e0004704.
18. Rukmana S et al. Molecular identification of trichoderma isolates from sugarcane bagasse based on internal transcribed spacer (ITS) rDNA. Research Journal of Pharmacy and Technology. 2020; 13(7):3300–4.
19. WHO. Comprehensive guidelines for prevention and control of dengue fever and dengue haemorrhagic fever. SEARO. World Health Organization New Delhi, India; 2011.
20. Sakurai A et al. Broad-spectrum detection of h5 subtype influenza a viruses with a new fluorescent immunochromatography system. PLoS One. 2013; 8(11):e76753.
21. Hotez PJ et al. Neglected tropical diseases among the Association of Southeast Asian Nations (ASEAN): overview and update. PLoS Negl Trop Dis. 2015; 9(4):e0003575.