Author(s):
Risma D. Manurung, Syafruddin Ilyas, Salomo Hutahaean, Rosidah rosidah, Putri C. Situmorang
Email(s):
syafruddin6@usu.ac.id
DOI:
10.52711/0974-360X.2022.00337 
Address:
Risma D. Manurung1, Syafruddin Ilyas1*, Salomo Hutahaean1, Rosidah rosidah2, Putri C. Situmorang1
1Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara, Medan, Indonesia, 20155.
2Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia, 20155.
*Corresponding Author
Published In:
Volume - 15,
Issue - 5,
Year - 2022
ABSTRACT:
Wounds of diabetic patients cause damage to skin tissue for a long time. Increased glucose levels affect angiogenesis which triggers the wound to heal. This study was to determine IL-1ß expression in diabetic wound healing after being given nano herbal of Zanthoxylum acanthopodium and Rhodomyrtus tomentosa. This study consisted of 4 treatment groups. K1: negative control, K2: MEBO, K3: Nano herbal of andaliman fruits (100mg/kg BW), and K4: Nano herbal of haramonting leaves (100mg/kg BW) on days 0, 4, 8, 12, and the 16th. The tissue was prepared on paraffin blocks and given Immunohistochemistry staining. Results showed that there was a significant difference between all groups (P <0.001) in IL-1ß expression. Histologically in wound healing, the granulation tissue of blood vessels is dominated by extensive fibroblasts and collagen. The epithelium has covered the wound that occurred on the skin. This suggests that some of the compounds contained in the nano herbal haramonting and andaliman can stimulate cell division and growth to form scar tissue. In conclusion, epithelium covered the wound in MEBO treatment, showing that the histology of the skin tissue was almost the same as that of nano herbal andaliman and haramonting. Therefore Nano herbal andaliman and haramonting can be developed into herbs that can be used to treat wounds in diabetics.
Cite this article:
Risma D. Manurung, Syafruddin Ilyas, Salomo Hutahaean, Rosidah rosidah, Putri C. Situmorang. Diabetic Wound Healing in IL-1β expression by Nano Herbal of Zanthoxylum acanthopodium and Rhodomyrtus tomentosa. Research Journal of Pharmacy and Technology. 2022; 15(5):2041-6. doi: 10.52711/0974-360X.2022.00337
Cite(Electronic):
Risma D. Manurung, Syafruddin Ilyas, Salomo Hutahaean, Rosidah rosidah, Putri C. Situmorang. Diabetic Wound Healing in IL-1β expression by Nano Herbal of Zanthoxylum acanthopodium and Rhodomyrtus tomentosa. Research Journal of Pharmacy and Technology. 2022; 15(5):2041-6. doi: 10.52711/0974-360X.2022.00337 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2022-15-5-19
REFERENCES:
1. Lolok N, Mashar HM, Annah I, Saleh A, Yuliastri WO, Isrul M. Antidiabetic Effect of the Combination of Garlic Peel Extract (Allium sativum) and Onion Peel (Allium cepa) in Rats with Oral-Glucose Tolerance Method. Research J. Pharm. and Tech. 2019; 12(5):2153-2156. doi: 10.5958/0974-360X.2019.00357.3
2. Jaiswal A, Senthil V, Das TK. Design and Development of Valsartan Loaded Nanostructured Lipid Carrier for the Treatment of Diabetic wound Healing. Research J. Pharm. and Tech. 2019; 12(6):2922-2928. doi: 10.5958/0974-360X.2019.00492.X
3. Kumudhaveni B, Radha R. Anti-diabetic potential of a traditional Polyherbal Formulation – A Review. Research J. Pharm. and Tech. 2017; 10(6): 1865-1869. doi: 10.5958/0974-360X.2017.00327.4
4. Sajid SR. Effect of Aegle marmelose Linn. Leaves in Dexamethasone Induced Insulin Resistance Diabetic Mice. Research J. Pharm. and Tech. 2015;8(3): 270-275. doi: 10.5958/0974-360X.2015.00045.1
5. Okonkwo U, DiPietro L. Diabetes and Wound Angiogenesis. Inter. J Mol. Sci. 2017;18(7):1419. doi:10.3390/ijms18071419
6. Shanmugapriya K, Kim H, Kang HK. A new alternative insight of nanoemulsion conjugated with κ-carrageenan for wound healing study in diabetic mice: In vitro and in vivo evaluation. Euro. J Pharmaceutical Sci.2019;133:236-250. doi: https://doi.org/10.1016/j.ejps.2019.04.006.
7. Ashwini D, Mahalingam G. Green Synthesized Metal Nanoparticles, Characterization and its Antidiabetic activities - A Review. Research J. Pharm. and Tech. 2020; 13(1): 468-474. doi: 10.5958/0974-360X.2020.00091.8
8. Basumatary S, Changmai N. Biological Materials Assisted Synthesis of Silver Nanoparticles and Potential Applications: A Review. Research J. Pharm. and Tech 2018; 11(6): 2681-2694. doi: 10.5958/0974-360X.2018.00497.3
9. Ayu A. Zuraidah, Winarni D, Punnapayak H, Darmanto W. Therapeutic Effect of Okra (Abelmoschus esculentus Moench) Pods Extract on Streptozotocin-Induced Type-2 Diabetic Mice. Research J. Pharm. and Tech 2019; 12(8):3703-3708. doi: 10.5958/0974-360X.2019.00633.4
10. Ponnanikajamideen M, Rajeshkumar S, Annadurai G. In Vivo Antidiabetic and in Vitro Antioxidant and Antimicrobial Activity of Aqueous Leaves Extract of Chamaecostus cuspidatus. Research J. Pharm. and Tech 2016; 9(8):1204-1210. doi: 10.5958/0974-360X.2016.00230.4
11. Surbhi Sharma M, Ashawat S, Solanki NS. Isotretinoin-Loaded Solid-Lipid Nanoparticles: A Sound Strategy for Skin Targeting. Research J. Pharm. and Tech 2012;5(10).
12. Yan C, Gao N, Sun H, Yin J, Lee P, Zhou L, et al. Targeting Imbalance between IL-1β and IL-1 Receptor Antagonist Ameliorates Delayed Epithelium Wound Healing in Diabetic Mouse Corneas. The American Journal of Pathology. 2016; 186(6):1466–1480. doi: https://doi.org/10.1016/j.ajpath.2016.01.019
13. Okizaki S, Ito Y, Hosono K, Oba K, Ohkubo H,Kojo K, et al. Vascular Endothelial Growth Factor Receptor Type 1 Signaling Prevents Delayed Wound Healing in Diabetes by Attenuating the Production of IL-1β by Recruited Macrophages. The American Journal of Pathology. 2016;186(6):1481–1498. doi:10.1016/j.ajpath.2016.02.014
14. Zhang YB, Li W, Jiang L, Yang L, Chen NH, Wu ZN, et al. Cytotoxic and anti-inflammatory active phloroglucinol derivatives from Rhodomyrtus tomentosa. Phytochemistry. 2018; 153:111–119. doi:10.1016/j.phytochem.2018.05.018
15. Irianti E, Ilyas S, Hutahaean S, Rosidah, Situmorang PC. Placental histological on Preeclamptic Rats (Rattus norvegicus) after administration of Nano herbal Haramonting (Rhodomyrtus tomentosa). Research J. Pharm. and Tech. 2020; 13(8):3879-3882. doi: 10.5958/0974-360X.2020.00686.1
16. Ilyas S, Hutahaean S, Rosidah R, Situmorang PC. Effects of Nano herbal Haramonting (Rhodomyrtus tomentosa) and Extra Virgin Olive Oil on Histology of Liver and Kidney of Preeclamptic Rats. Pak.J.Biol.Sci., 2020;23(12):1629-1635. doi:10.3923/pjbs.2020.1629.1635
17. Ilyas S, Murdella F, Hutahaean S, Situmorang PC. The Effect of Haramounting Leaf Ethanol Extract (Rhodomyrtus tomentosa (Aiton) Hassk.) on the Number of Leukocyte type and Histology of Rats Pulmo (Mus Musculus L.) Exposed to Electronic Cigarette. Open.Access. Maced. J Med. Sci. 2019;7: 1750–1756. doi: 10.3889/oamjms.2019.467
18. Ilyas S. Description of Kidney Histology of Mus Musculus After Giving Nano Hebal Rhodomyrtus tomentosa (Haramounting). International Journal of Ecophysiology. 2019;1(1),:26-33.doi: https://doi.org/10.32734/ijoep.v1i1.844
19. Ilyas S. Effect of Nano herbal Haramounting (Rhodomyrtus tomentosa) in Histology of Heart Mus musculus. International Journal of Ecophysiology. 2019: 1(2):101-106. doi: https://doi.org/10.32734/ijoep.v1i2.1272
20. Ilyas S. 2020. Effect of Nano herbal Haramonting (Rhodomyrtus tomentosa) on Lungs Histology. International Journal of Ecophysiology. 2020; 2(1):43-47. doi: https://doi.org/10.32734/ijoep.v2i1.3992
21. Ilyas S. Histology of Spleen after Induction Nano herbal Rhodomyrtus tomentosa. International Journal of Ecophysiology. 2020;2(1): 70-74. doi: https://doi.org/10.32734/ijoep.v2i1.3993
22. Situmorang PC, Ilyas S. Description of Testis Histology Of Mus Musculus After Giving Nano Herbal Rhodomyrtus tomentosa (Haramonting). Asian J Pharm Clin Res. 2018;11:461-463. doi: http://dx.doi.org/10.22159/ajpcr.2018.v11i11.29042
23. Situmorang PC, Ilyas S, Hutahaean S. Study of Combination of Nano herbal Andaliman (Zanthoxylum acanthopodium) and Extra Virgin Olive Oil (EVOO) effects in the Expression of MDA, HSP-70 and Placental Histology of Preeclamptic Rats. Pharm. Sci. 2019; 25(3): 205-220. doi: 10.15171/PS.2019.37
24. Situmorang PC, Ilyas S, Hutahaean S, Rosidah R, Manurung RD. Acute toxicity Test and Histological Description of Organs after Giving Nano Herbal Andaliman (Zanthoxylum acanthopodium). Rasayan J. Chem. 2020;13(2):780-788. doi: http://dx.doi.org/10.31788/RJC.2020.1325621
25. Situmorang PC, Ilyas S ,Hutahaean S. Effect of Combination of Nano Herbal Haramonting (Zanthoxylum acanthopodium DC.) and Extra Virgin Olive Oil (EVOO) to Kidney Histology of Preeclampsia Rat. IOP Conf.Series:Earth and Environ.Sci. 2019; 305:012081. doi:10.1088/1755-1315/305/1/012081
26. Situmorang PC, Ilyas S. Hutahaean S, Rosidah R. Effect of Nano herbal andaliman (Zanthoxylum acanthopodium) and Extra Virgin Olive Oil Combination on Preeclamptic Rats Liver Histology. Open.Access.Maced.J.Med Sci.2019;7:2226-2231. doi:10.3889/oamjms.2019.651
27. Situmorang PC, Ilyas S, Hutahaean S, Rosidah R. Components and acute toxicity of nano herbal haramonting (Rhodomyrtus tomentosa). J Herbmed Pharmacol. 2021;10(1):139-148. doi: 10.34172/jhp.2021.15.
28. Fu H, Wang S, Ge B, Li L, Zeng H, Shu Q, et al. Nerve growth factor and substance P may be involved in moist exposed burn ointment mediated chronic refractory wound healing. Experimental and Therapeutic Medicine. 2018; 16: 1987-1993. https://doi.org/10.3892/etm.2018.6390
29. Minutti CM, Knipper JA, Allen JE, Zaiss DMW. Tissue-specific contribution of macrophages to wound healing. Seminars in Cell & Developmental Biology.2017; 61-3–11. doi:10.1016/j.semcdb.2016.08.006
30. De Lorenzo G, Ferrari S, Cervone F, Okun E.Extracellular DAMPs in Plants and Mammals: Immunity, Tissue Damage and Repair. Trends in Immunology. 2018;39;11:937-950. doi:10.1016/j.it.2018.09.006
31. Peng J, Zheng H, Wang X, Cheng Z. Upregulation of TLR4 via PKC activation contributes to impaired wound healing in high-glucose-treated kidney proximal tubular cells. PLOS ONE. 2017;12(5): e0178147. doi:10.1371/journal.pone.0178147
32. Wada J, Makino H. Innate immunity in diabetes and diabetic nephropathy. Nat.Rev. Nephrol.2016; 12:13–26. doi:https://doi.org/10.1038/nrneph.2015.175
33. Hu H, Lee SR, Bai H, Guo J, Hashimoto T, Isaji T,et al. TGFβ (Transforming Growth Factor-Beta)–Activated Kinase 1 Regulates Arteriovenous Fistula Maturation. Arteriosclerosis, Thrombosis, and Vascular Biology.2020; 40:e203–e213. doi:10.1161/atvbaha.119.313848
34. Wang R, Pu H, Ye Q, Jiang M, Chen J, Zhao J,Shi, Y. Transforming Growth Factor Beta-Activated Kinase 1–Dependent Microglial and Macrophage Responses Aggravate Long-Term Outcomes After Ischemic Stroke. Stroke.2020;51:975-985.doi:10.1161/strokeaha.119.028398
35. Sujatha Kumari M, Kiran Babu M, Sulthana R, Srinivas M, Prasanthi CH. Diabetes Mellitus: Present status and Drug therapy Updates. Research J. Pharm. and Tech.2014; 7(1): 84-94.
36. Nag Mk, Patel S, Shrivastava S, Daharwal SJ, Singh MR, Singh S. An Overview on Diabetes Mellitus with Emphasis on Non Invasive Techniques of Insulin Delivery. Research J. Science and Tech. 2013;5(4): 387-395.
37. Singh S, Jangde R,Daharwal SJ. An Updated Review on Herbal Drug in Wound Healing. Research J. Pharm. and Tech. 2019; 12(6): 3089-3097. doi: 10.5958/0974-360X.2019.00523.7
38. Sahu P, Jangade R. An Updated Review on Mechanism of Novel Carrier System for Wound Healing. Research J. Topical and Cosmetic Sci. 2019;10(2): 65-78. doi: 10.5958/2321-5844.2019.00014.1
39. Ayush Jaiswal, V. Senthil,Tamal Kusum Das. Design and Development of Valsartan Loaded Nanostructured Lipid Carrier for the Treatment of Diabetic wound Healing. Research J. Pharm. and Tech. 2019; 12(6):2922-2928. doi: 10.5958/0974-360X.2019.00492.X
40. Sayeed ul hasan S, Nitin Mahurkar M. Evaluation of Wound Healing Effect of Polyherbal Ointment in Alloxan Induced Diabetic Rats. Research J. Topical and Cosmetic Sci. 2015;6(1): 38-43. doi: 10.5958/2321-5844.2015.00006.0
41. Sarma B, Goswami BCH. Evaluation of Hypoglycemic effect of Ethanol extract of Musa paradisiaca unripe fruit pulp on normal and alloxan induced diabetic mice. Research J. Pharm. and Tech. 2018; 11(3): 1048-1052. doi: 10.5958/0974-360X.2018.00196.
42. Rudhanton D, Wulan PMY, Yudianto DO. The Beneficence from Gel of Ambon Banana (Musa Paradisiaca Var. Sapientum) to increase Wound Healing Post-Gingivectomy in Wistar Rat (Rattus Norvegicus)- In vivo Study. Research J. Science and Tech. 2019; 11(2):89-93. doi: 10.5958/2349-2988.2019.00014.7
43. Weremfo A, Pappoe ANM, Adinortey MB. Wound Healing Potential of Musa paradisiaca L. (Musaceae) stem juice extract formulated into an ointment. Res. J. Topical and Cosmetic Sci. 2011;2(2): 71-73
44. Senthilnathan B, Vivekanandan K, Bhavya E, Masilamani, Swarna Priya B. Impact of Nanoparticulate Drug Delivery System of Herbal Drug in Control of Diabetes Mellitus. Research J. Pharm. and Tech. 2019; 12(4): 1688-1694. doi: 10.5958/0974-360X.2019.00282.8
45. Rezzola S, Loda A, Corsini M, Semeraro F, Annese T, Presta M, Ribatti D. Angiogenesis-Inflammation Cross Talk in Diabetic Retinopathy: Novel Insights From the Chick Embryo Chorioallantoic Membrane/Human Vitreous Platform. Frontiers in Immunology. 2020; 11. doi:10.3389/fimmu.2020.581288
46. Bezold V, Rosenstock P, Scheffler J, Geyer H, Horstkorte R, Bork K. Glycation of macrophages induces expression of pro-inflammatory cytokines and reduces phagocytic efficiency. Aging (Albany NY). 2019;11(14):5258-5275. doi:10.18632/aging.102123
47. Tan BL, Norhaizan ME, Liew, WPP, Sulaiman Rahman H. Antioxidant and Oxidative Stress: A Mutual Interplay in Age-Related Diseases. Frontiers in Pharmacology. 2018; 9. doi:10.3389/fphar.2018.01162
48. Farahpour MR. Medicinal Plants in Wound Healing, Wound Healing - Current Perspectives, Kamil Hakan Dogan, IntechOpen. 2019. doi: 10.5772/intechopen.80215.
49. Wijaya CH, Napitupulu FI, Karnady V, Indariani S. A review of the bioactivity and flavor properties of the exotic spice “andaliman” (Zanthoxylum acanthopodium DC.). Food Rev Inter. 2019;35;1:1–19. doi:https://doi.org/10.1080/87559129.2018.1438470