Background: Olive oil is a natural source of phytoestrogens and antioxidants similar to mammalian estrogen. The substance increases osteoblast differentiation and osteoprotegerin (OPG) expression. Objective of the study: To examine the effect of giving olive oil on OPG expression of gingival crevicular fluid in the orthodontic tooth movement among old and young guinea pigs. Methodology: The four groups of guinea pigs (n = 3) consisted of old guinea pigs given olive oil (OO), old controls (OC), the young given olive oil (YO), and young controls (YC). The teeth were movedreciprocally to distal by a 0.35 N open coil spring which was attached to the bracket of the lower incisors. The YO group was given olive oil at 0.7ml/day dosage and the OO group had 1.86ml olive oil administered per day until day 14. OPG expression was analyzed in gingival crevicular fluid (GCF) sample using the enzyme-linked immunosorbent assay (ELISA) on days 0, 7, and 14. Retrieved data was analyzed using Three-way ANOVA Statistic Test followed by Post Hoc LSD Test. Results: The statistical test showed that olive oil increased OPG expression in old and young guinea pigs (p<0.05), and OPG OO expression was comparable to that in YC day 7. OPG YO expression was higher than OO (p <0.05). Increasing duration of olive oil application led to higher OPG expression (p <0.05). There was a correlation between olive oil and age and duration of administration (p <0.05). Conclusion: Olive oil increased the OPG expression on GCF of old and young guinea pigs during orthodontic tooth movement, with the highest OPG expression in YO day 14.
Cite this article:
Dina Listyowati, Sri Suparwitri, Cendrawasih Andusyana Farmasyanti. Effect of Olive oil on Osteoprotegerin Expression on the Tension side of Gingival Crevicular Fluid in the Orthodontic Tooth Movement of Old and Young Guinea Pigs. Research Journal of Pharmacy and Technology. 2022; 15(6):2621-6. doi: 10.52711/0974-360X.2022.00438
Dina Listyowati, Sri Suparwitri, Cendrawasih Andusyana Farmasyanti. Effect of Olive oil on Osteoprotegerin Expression on the Tension side of Gingival Crevicular Fluid in the Orthodontic Tooth Movement of Old and Young Guinea Pigs. Research Journal of Pharmacy and Technology. 2022; 15(6):2621-6. doi: 10.52711/0974-360X.2022.00438 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2022-15-6-42
1. Wise GE, King GJ, Mechanisms of tooth eruption and orthodontic tooth movement, J Dent Res,2008;87: 414–21.
2. Alhasyimi AA, Pudyani PS, Asmara W, Ana ID, Enhancement of post-orthodontic tooth stability by carbonated hydroxyapatite-incorporated advanced platelet-rich fibrin in rabbits, Orthod Craniofac Res, 2018;21(2):112–8.
3. Hofbauer LC, Heufelder AE, Role of receptor activators of nuclear factor-kappaB ligand and osteoprotegerin in bone cell biology, J Mol Med, 2001;79(5):243–53.
4. Alhasyimi AA, Pudyani PS, Asmara W, Ana ID, Locally inhibition of orthodontic relapse by injection of carbonated hydroxy apatite-advanced platelet rich fibrin in a rabbit model, Key Eng Mater 2017;758:255–63.
5. Boyce BF, Xing L, Function of RANKL / RANK / OPG in bone modeling and remodeling, Arch Biochem Biophys. 2008;473(2):139–46.
6. Khrisnan V, Davidovitch Z, Cellular, Molecular, and Tissue Level Reactions to Orthodontic Force. Am J Orthod Dentofacial Orthop, 2006;129 (4): 460-68.
7. King GJ, Keeling SD, 1995, Orthodontic bone remodeling in relation to appliance decay, Angle Orthod, 1995;65 (2): 129-40.
8. Mukherjee, Nayak K, Nayak A, Adarsh, Variations of salivary level of osteoprotegerin during osthodontic tooth movement, J Indians Orthod, 2019; 53(1): 10-13.
9. Suparwitri S, Niswati FR, Alhasyimi AA, Wheat seeds can delay orthodontic tooth movement by blocking osteogenesis in rats, Clin Cosmet Investig Dent, 2019; 11: 243–249.
10. Rivas A, Romero A, Mariscal M, Monteagudo C, Herna'ndez J, Olea-Serrano F, Validation of questionnaire for the study of food habits and bone mass, Nutr Hosp,2009;24 (5): 521-28.
11. Bonjour JP, Protein intake and bone health, Int J Vitam Nutr Res, 2011;81 (2): 134-42.
12. Marwaha RK, PuriS, Tandon N, Dhir S, Agarwal N, Bhadra K, Saini N, Effects of sports training and nutrition on bone mineral density in young indian healthy female, IndiaJ Med Re,2011;134 (3): 307-13.
13. Keiler AM, Zierau O, Bernhardt R, Scharnweber D, Lemonakis N, Ternetzi A. et al, Impact of A Functionalized Olive oil Extract on The Uterus and The Bone in A Model of Postmenopausal Osteoporosis, Eur JNutr, 2014; 53 (4): 1073-81.
14. Zerzour A, Hadding NE, Derouiche S, Analysis of osteoporosis risk factors in menopausal women’s of Algeria population, Asian J. Res. Pharm. Sci. 2020; 10(2):79-84.
15. Patel VK, Kpatel C, Patel HU, Patel CN, Vitamins, minerals and carotenoids as a antioxidants, Asian J. Research Chem. 3(2): April- June 2010; Page 255-260.
16. Jaydeokar AV, Bandawane DD, Nipate SS, Chaudhari PD, Natural antioxidants: a review on therapeutic applications, Research J. Pharmacology and Pharmacodynamics. 4(1): Jan. - Feb., 2012, 55-61
17. Tiwari P, Patel RK, Estimation of total phenolics and flavonoids and antioxidant potential of ashwagandharishta prepared by traditional and modern methods, Asian J. Pharm. Ana. 3(4): Oct. - Dec. 2013; Page 147-152.
18. Ashfaq MH, Siddique A, Shahid S, Antioxidant activity of cinnamon zeylanicum: (A review), Asian Journal of Pharmaceutical Research. 2021; 11(2):106-116.
19. Pérez-Jiménez F, Ruano J, Perez-Martinez P, Lopez-Segura F, Lopez-Miranda J, 2007, The influence of olive oil on human health: Not a question of fat alone, Mol Nutr Food Res, 2007; 51, 1199-206.
20. Brown L, Poudyal H, Panchal, SK, Functional food as a potential therapeutic options for metabolic syndrome, Obes. Rev, 2015:16 (11): 914-41.
21. Harshitha C, Murthykumar K, Deepak A, Beneficial effect of olive oil on human health – A review, Research J. Pharm. and Tech. 2016; 9(5): 593-595.
22. Khobragade CN, Bodade RG, Manwar AV, Synthesis and antioxidant activity of some flavone derivatives, Asian J. Research Chem. 3(1): Jan.-Mar. 2010; Page 139-141
23. Thompson L, Boucher B, Lui Z, Cotterchio M, Kreiger N, Phytoestrogen content of foods consumed in Canada, including isoflavones, ligans and coumestan, Nutrition and cancer, 2006;54 (2): 184-201.
24. LiuH, Huang H, Li B, Wu D, Wang F, Zheng XH et al, Olive oil in the prevention and treatment of osteoporosis after artificial menopouse, Clin Interv Aging, 2014; 9, 2087-95.
25. OstrowskaE, Gabler NK, Ridley D, Suster D, Eangling DR, DunsheaFR, Extra virgin and refined olive oils decrease plasma triglyceride, moderately affect lipoprotein oxidation susceptibility and increase bone density in growing pigs, JSci FoodAgric, 2006; 86 (22): 1955-62.
26. Ren Y, Maltha JC, Van't Hof MA, Kuijpers-Jagtman AM, Age effect on orthodontic tooth movement in rats, J Dent Res, 2003; 82 (1): 38-42.
27. Shobha S, Rao MD, Nitin, BMD, Ambreen A, Osteoporosis in men. Article, Am Fam Physician, 2010; 82 (5): 503-10.
28. Gruber CJ, Tschugguel W, Schneeberger C, Huber CJ, Production and actions of estrogens, N Engl J Med, 2002; 346 (5): 340-52.
29. Hofbauer LC, Lacey DL, Dunstan CR, Spelsberg TC, Riggs BL, Khosla S, Interleukin-1beta and tumor necrosis factor-alpha, but not interleukin-6, stimulate osteoprotegerin ligand gene expression in human osteoblasic cells, J Bone, 1999; 25: 255–59.
30. Dr. Chitra V, Ali MA, Animal models for osteoporosis – A review, Research J. Pharm. and Tech 2020; 13(3):1543-1548.
31. Radhakrishna B, Ashok M, Harish PL, Joythsna M, Gowda SKP, Current and future trends of drugs used in osteoporosis, Research J. Pharmacology and Pharmacodynamics. 3(6): Nov.-Dec., 2011, 329-333.
32. Kim, Tabata A, Tomoyasu Tet al, Estrogen stimuli promote osteoblasic differentiation via the subtilisin-like proprotein convertase PACE4 in MC3T3-E1 cells, J Bone Miner Metab, 2015; 33 (1): 30-39.
33. Jiménez, Ruano J, Perez-Martinez P, Lopez-Segura F, Lopez-MirandaJ, The influence of olive oil on human health: Not a question of fat alone, Mole Nutr Food Res, 2007; 51 (10): 1199–208.
34. Irianti E, Ilyas S, Hutahaen S, Rosidah, The role of EVOO (extra virgin olive oil) in serum MDA concetration and blood pressure of pregnant mice (rattus norvegicus) with ple-eclampsia, Research J. Pharm. and Tech. 2018; 11(3):1053-1057.
35. BanfiG, Iorio EL, Corsi, MM, Oxidative stress, free radicals and bone remodeling, Clin Chem Lab Med, 2008; 46; 1550–64.
36. Romagnoli C, Marcucci G, Favilli F, Zonefrati R, Mavilia C, Galli G, Tanini A, Iantomasi T, Brandi ML, Vincenzini MT, Role of GSH / GSSG redox couple in osteogenic activity and osteoclastogenic markers of human osteoblast-like SaOS-2 cells, FEBS J 2013; 280: 867–79
37. Fontani F, Marcucci G, Iantomasi T, Brandi ML, Vincenzini MT, Glutathione, N-acetylcysteine and lipoic acid down-regulate starvation-induced apoptosis, RANKL / OPG ratio and sclerostin in osteocytes: involvement of JNK and ERK1 / 2 signaling, Calcif Tissue Int, 2015; 96: 335–46.
38. IkedaT, Utsuyama M, Hirokawa K, Expression profiles of receptor activator of nuclear factor kappaB ligand, receptor activator of nuclear factor kappa B, and osteoprotegerin messenger RNA in aged and ovariectomized rat bones, JBone Miner Res, 2001; 16 (8): 1416–25.
39. Cao, Venton L, Sakata T, Expression of RANKL and OPG correlates with age-related bone loss in male C57BL / 6 mice. J Bone Miner Res, 2003; 18 (2): 270-6.
40. Miro-Casas E, Albaladejo MF, Covas MI, Rodriguez JO, Colomer EM, Raventos RML, Hydroxitirosol disposition in humans, Clinical Chemistry, 2003; 49: 945-52.
41. KendallM, Batterham M, Prenzler PD, Ryan D, Robards K, Absorbtion, Metabolism and Excretion of Phenols Derived from Olive Products, Functional Plant Science and Biotechnology 2009; (3): 81-91.
42. Tan, Ren Y, Wang J, Jiang L, Cheng H, Sandham A, Zhao Z, Osteoprotegerin and Ligand of Receptor Activator of Nuclear Factor, Angle Orthod,2009;79(2): 292-99.