Author(s): Chandra Shakar Reddy Nallagouni, Pratap Reddy Karnati


DOI: 10.52711/0974-360X.2022.00439   

Address: Chandra Shakar Reddy Nallagouni, Pratap Reddy Karnati*
Dept of Zoology, UCS, Osmania University, TS, India.
*Corresponding Author

Published In:   Volume - 15,      Issue - 6,     Year - 2022

The present study aimed to investigate the ameliorative effects of Selenium and Alpha-tocopherol on activities of enzymes involved in metabolic functions, energy production and transfer in the brain and gastrocnemius muscle of mice treated with sodium fluoride. Animals were separated into five groups and treated with Selenium (05 µg/kg BW), Alpha-tocopherol (2mg/kg BW) alone, and in combination for 15 days to evaluate their ameliorative effects against fluoride (20mg/kg BW) toxicity. The obtained results showed a statistically significant (p<0.05) decrease in the body weight, organo-somatic index, protein content, ALAT, AAT, CPK, acid phosphatase and alkaline phosphatase enzymes activities in the fluoride-treated group in comparison with control group indicates oxidative stress induced by fluoride. The effect of fluoride on enzymes of muscle was comparatively higher, which suggests the greater accumulation of fluoride in muscle than brain. These changes were reverted moderately in Selenium and alpha-tocopherol alone treated groups, and significantly (p<0.05) in Selenium + alpha-tocopherol treated group suggests the combinatorial effect of Selenium and alpha-tocopherol in the amelioration of fluoride-induced toxicity. In conclusion, the findings all pointed to the fact that Selenium and alpha-tocopherol showed combinatorial effects in the amelioration of oxidative stress induced by fluoride.

Cite this article:
Chandra Shakar Reddy Nallagouni, Pratap Reddy Karnati. Effects of Fluoride on Metabolic enzymes of brain and Gastrocnemius muscle of mice: protective role of selenium and alpha-tocopherol. Research Journal of Pharmacy and Technology. 2022; 15(6):2627-2. doi: 10.52711/0974-360X.2022.00439

Chandra Shakar Reddy Nallagouni, Pratap Reddy Karnati. Effects of Fluoride on Metabolic enzymes of brain and Gastrocnemius muscle of mice: protective role of selenium and alpha-tocopherol. Research Journal of Pharmacy and Technology. 2022; 15(6):2627-2. doi: 10.52711/0974-360X.2022.00439   Available on:

1.    Johnston NR. Strobel SA. Principles of fluoride toxicity and the cellular response: a review. Arch Toxicol. 2020; 94: 1051–1069. doi: 10.1007/s00204-020-02687-5.
2.    Chowdhury CR. Shahnawaz K. Kumari D. Chowdhury A. Bedi R. Lynch E. Harding S. Grootveld M. Spatial distribution mapping of drinking water fluoride levels in Karnataka, India: fluoride related health effects. Perspect Public Health. 2016; 136:353–360. doi: 10.1177/1757913915626744.
3.    Kinawy AA. Ezzat AR. Impact of aluminum and antioxidants on some neural aspects. Lambert Academic Publishing, Riga. 2013; 1-124. ISBN-13:978-3659410888.
4.    Sugavasi Raju. Senthil Kumar Sivanesan. Kanchanalatha Gudemalla. Ravi Mundugaru. Madhankumar Swaminathan. Effect of Ginkgo biloba extract on Hematological and Biochemical Alterations in Fluoride Toxicity in Wistar rats. Research J. Pharm. and Tech 2019; 12(8):3915-3918. doi:10.5958/0974-360X.2019.00674.7
5.    Bhuvaneswari G, Gokulapriya P. Assess the Dental fluorosis and associated risk factors among adolescence, living in high level Fluoride content in drinking water at selected community area. Research J. Pharm. and Tech 2018; 11(10): 4237-4241. doi: 10.5958/0974-360X.2018.00776.X
6.    Chaitali Sarkar. Sudipta Pal. Niranjan Das. Biswanath Dinda. Ameliorative effects of oleanolic acid on fluoride induced metabolic and oxidative dysfunctions in rat brain: Experimental and biochemical studies. Food and Chemical Toxicology. 2014; 66: 224–236. doi: 10.1016/j.fct.2014.01.020.
7.    Vani ML. Pratap Reddy K. Effects of fluoride accumulation on some enzymes of brain and gastrocnemius muscle of mice. Fluoride. 2000; 33(1):17-26.
8.    Sonam Soni. Pravin Kumar. Vinay Sagar Verma. Mukesh Sharma. Significance of Indian Medicinal Plants used for Treatment of Dementia. Asian J. Res. Pharm. Sci. 2014; 4(4): 202-205. doi: 10.52711/2231-5659.
9.    Hanaa E Mahood. Baydaa M Abbood. Physiological and Histological Study of Preventive effect of Antioxidants, Selenium and Vitamin C with Garlic in Rats treated with Sodium Fluoride. Research J. Pharm. and Tech. 2018; 11(3): 941-945. doi: 10.5958/0974-360X.2018.00174.9
10.    Swati Korabu. Rajani Nair. Chaitrali Pawar. Rohit Shah. Priyanka Jadhav. Bhakti Chorghe. Selenium as an Antioxidant: A Review. Asian J. Research Chem. 2013; 6(3): 278-285. doi: 10.5958/0974-4150
11.    Muller DPR. Lloyd JK. Wolff OH. Vitamin E and neurological function Lancet. 1983; 29;1(8318):225-8.
12.    Towfighi J. Effects of chronic vitamin E deficiency on the nervous system of the rat. Acta Neuropath.1981; 54: 261-267. doi: 10.1007/BF00696998.
13.    Jiang Q. Natural forms of vitamin E: Metabolism, antioxidant, and anti-inflammatory activities and their role in disease prevention and therapy. Free Radic Biol Med. 2014;72: 76–90. doi: 10.1016/j.freeradbiomed.2014.03.035.
14.    Saher Mahmood Jwad. Bushra Abbas. Haider S. Jaffat. Study of the Protective Effect of Vitamin C plus E on Lincomycin-Induced Hepatotoxicity and Nephrotoxicity. Research J. Pharm. and Tech.2015; 8(2):177-184.doi: 10.5958/0974-360X.2015.00032.3.
15.    Thangavel K. Selenium Properties for Anti-Cancer. Research J. Pharm. and Tech. 2017; 10(10): 3595-3597.doi: 10.5958/0974-360X.2017.00651.5.
16.    Hamid AA. Aiyelaagbe OO. Usman LA. Ameen OM. Lawal A. Antioxidants: Its medicinal and pharmacological applications. African Journal of Pure and Applied Chemistry. 2010; 4(8): 142-151.
17.    Afyaa Sabah Nasir. Haider Salih Jaffat. Symbiotic effect of Sodium Selenite and Vitamin E against Lithium Carbonate induced Brain Toxicity in Male Rats. Research J. Pharm. and Tech. 2017; 10(6): 1661-1665.doi: 10.5958/0974-360X.2017.00293.1.
18.    Rakhi Menon. Antioxidants and their Therapeutic Potential- A Review. Research J. Pharm. and Tech. 2013; 6(12):1426-1429.doi: 10.5958/0974-360X.
19.    Krishnaiah C. Reddy KP. Dose-dependent effects of fluoride on neurochemical milieu in the hippocampus and neocortex of rat brain. Fluoride. 2007; 40: 101–110.
20.    Lowry OH. Rosebrough NJ. Farr AL. Randall RJ. Protein measurement with the Folin phenol reagent. J. Biol. Chem.1951; 193: 265. PMID: 14907713.
21.    Gerhardt W. Waldenstrom J. Gruber W. EDTA effect of creatine kinase (CK) and on the SCE reagent. Scand J Clin Lab Invest 1979; 39:737-42.doi: 10.1080/00365517909108165.
22.    Retiman. Frankel. A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. Am J Clin Pathol 1957; 28(1):56-63.doi: 10.1093/ajcp/28.1.56.
23.    Taussky HH. eshorr A. Microcolorimetric method for the determination of inorganic phosphorus. J biol chem.1953; 202(2):675-85. PMID: 13061491.
24.    Sudhakar K. Nageshwar M. Reddy KP. Protective effect of Okra, Abelmoschus moschatus seed extract on developing brain of rats during pre- and post-natal fluoride exposure. Int J Pharm Sci & Res. 2018; 9(4): 1519-28.doi: 10.13040/IJPSR.0975-8232.9(4).1519-28
25.    Trivedi MH. Verma RJ. Sangai NP. Chinoy NJ. Mitigation by black tea extract of sodium fluoride-induced histopathological changes in brain of mice. Fluoride. 2012; 45: 13–26.
26.    Mohsen Meydani. John B Macauley. Jeffrey B. Blumberg Effect of Dietary Vitamin E and Selenium on Susceptibility of Brain Regions to Lipid Peroxidation. Lipids. 1988; 23(5): 405-409. doi: 10.1007/BF02535510.
27.    Tandra Das T. Role of Antioxidants in Health and Diseases-A Review. Research J. Pharm. and Tech. 2015; 8(8): 1033-1037.doi: 10.5958/0974-360X.2015.00176.6.

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