Author(s): Adnan Ali Hammad, Ayman A. Farrag, Tarek M. Abdelghany, Amr A. El-Waseif, Saleem Obaid Gatia Almawla


DOI: 10.52711/0974-360X.2022.00528   

Address: Adnan Ali Hammad1,2, Ayman A. Farrag2,3, Tarek M. Abdelghany2, Amr A. El-Waseif2, Saleem Obaid Gatia Almawla4
1Anbar Education Directorate, Iraqi Ministry of Education, Anbar, Iraq.
2Botany and Microbiology Dept., Faculty of Science (Boys), Al-Azhar University, Cairo, Egypt.
3Al-Azhar Center for Fermentation Biotechnology and Applied Microbiology Al-Azhar University, 11884 Nasr City, Cairo, Egypt.
4Alanbar general Health directorate, Ministry of health, Anbar, Iraq.
*Corresponding Author

Published In:   Volume - 15,      Issue - 7,     Year - 2022

Plant extracts promise natural sources of antivirulence chemicals thanks to their broad availability and various phytochemical antivirulence components. The main antivirulence components of plants are essential oils. 130 isolates were obtained from neonates (=1 year old) from February, 2020 to February, 2021. All studied isolates were diagnosed by traditional methods like biochemical tests, Vitek-2 system, and 16srRNA using conventional PCR. Profile of antibiotics resistance ratio for Streptococcus agalactiae were Penicillin 20%, Clindamycin 68%, Chloramphenicol 46%, Tetracycline 82%, Erythromycin 100%, Imipenem 12%, and Ciprofloxacillin 26.47%. All genes (Cyl E, Spb1, and bibA) were detected by conventional PCR and the molecular frequency showed 64% of strains positive for Cyl E gene and 56% of strains harbored bibA genes, while 48% of Streptococcus agalactiae contain Spb1 gene. This research showed the coexistence of bla Cyl E, Spb 1 and bibA genes in 48% strains of Streptococcus agalactiae. Extraction of clove extract was carried out using methanol and it analysed using GC-MS to detect active compounds, such as Eugenol (60.10%). The effect of clove extract against biofilm formation, haemolysin, and protease activity was significant (p-value < 0.01).

Cite this article:
Adnan Ali Hammad, Ayman A. Farrag, Tarek M. Abdelghany, Amr A. El-Waseif, Saleem Obaid Gatia Almawla. Correlation Study of Alcoholic Clove extract against Streptococcus agalactiae virulence factors isolated from Neonatal patients. Research Journal of Pharmacy and Technology. 2022; 15(7):3159-4. doi: 10.52711/0974-360X.2022.00528

Adnan Ali Hammad, Ayman A. Farrag, Tarek M. Abdelghany, Amr A. El-Waseif, Saleem Obaid Gatia Almawla. Correlation Study of Alcoholic Clove extract against Streptococcus agalactiae virulence factors isolated from Neonatal patients. Research Journal of Pharmacy and Technology. 2022; 15(7):3159-4. doi: 10.52711/0974-360X.2022.00528   Available on:

1.    Sørensen UB. Klaas IC. Boes J. Farre M. The distribution of clones of Streptococcus agalactiae (group B streptococci) among herdspersons and dairy cows demonstrates lack of host specificity for some lineages. Veterinary microbiology. 2019; 235: 71-79:
2.    Chen B. Chen H. Shu X. Yin Y.  Li J. Qin J. Xiang C. Presence of segmented filamentous bacteria in human children and its potential role in the modulation of human gut immunity. Frontiers in microbiology. 2018; 9: 1403:
3.    Gamal A. El-Noby Hassanin M. El-Hady M. Aboshabana S. Streptococcus: A review article on an emerging pathogen of farmed fishes.  Egyptian Journal of Aquatic Biology and Fisheries. 2021; 25.1: 123-139: doi: 10.21608/ejabf.2021.138469
4.    Motallebirad T. Fazeli H. Ghahiri A. Shokri D. Jalalifar S. Moghim S. Esfahani BN.  Prevalence, population structure, distribution of serotypes, pilus islands and resistance genes among erythromycin-resistant colonizing and invasive Streptococcus agalactiae isolates recovered from pregnant and non-pregnant women in Isfahan, Iran. BMC microbiology. 2021; 21.1: 1-11:
5.    Subramanian S. Shenoy PA. Pai V. Antimicrobial activity of some Essential oils and Extracts from Natural sources on Skin and Soft tissue infection causing microbes: An In-vitro Study. Research Journal of Pharmacy and Technology. 2021; 14.7: 3603-3609: doi: 10.52711/0974-360X.2021.00623
6.    Puopolo KM. Lynfaield R. Cummings JJ. Management of infants at risk for group B streptococcal disease. Pediatrics. 2019; 144.2:
7.    Abbas HA. Inhibition of virulence of Pseudomonas aeruginosa: a novel role of metronidazole against aerobic bacteria. Research Journal of Pharmacy and Technology. 2015; 8.12: 1640-1644: doi : 10.5958/0974-360X.2015.00295.4
8.    Altaif KI. Zharif DM. Hailat IA. Alqaisi KM. Al-Sultan II. A Study on Antibacterial sensitivity character to Piper betle: A Potential for alternative medicine. Research Journal of Pharmacy and Technology. 2021;14(9), 4920-4924: doi: 10.52711/0974-360X.2021.00855
9.    Kandhan TS. Roy A. Lakshmi T. Rajeshkumar S. Green synthesis of Rosemary oleoresin mediated silver nanoparticles and its effect on Oral pathogens. Research Journal of Pharmacy and Technology. 2019; 12.11: 5379-5382: doi : 10.5958/0974-360X.2019.00933.8
10.    Zhang D. Gan RY. Zhang JR. Farha AK. Li HB. Zhu F. Corke H. Antivirulence properties and related mechanisms of spice essential oils: A comprehensive review. Comprehensive Reviews in Food Science and Food Safety.2020; 19(3), 1018-1055:
11.    Mary R. Banu N. Inhibition of Antibiofilm Mediated Virulence Factors in Pseudomonas aeruginosa by Andrographis Paniculata. Research Journal of Pharmacy and Technology.2017; 10(1), 141-144: doi : 10.5958/0974-360X.2017.00031.2
12.    Phuoc NN. Linh NT. Crestani C. Zadoks RN. Effect of strain and enviromental conditions on the virulence of Streptococcus agalactiae (Group B Streptococcus; GBS) in red tilapia (Oreochromis sp.). Aquaculture. 2021; 534, 736256:
13.    Melo-Cristino J. Fernandes ML. Portuguese Surveillance group for the study of Respiratory Pathogens, Streptococcus pyogenes Isolated in Portugal: Macrolide Resistance Phenotypes and Correlation with T Types. Microbial drug resistance.1999; 5(3), 219-225:
14.    karm IF. Investigation of active compound in Clove (Syzygium aromaticum) Extrac and compared with inhibitors of growth of some types of Bacteria causing food poisoning. Iraqi journal of agricultural sciences. 2019; 50 (6):
15.    Yuldasheva  LN. Carvalho EB. Catanho MT. Krasilnikov OV. Cholesterol-dependent hemolytic activity of Passiflora quadrangularis leaves. Brazilian Journal of Medical and Biological Research. 2005; 38, 1061-1070: doi: 10.1590/s0100-879x2005000700009
16.    Krasilnikov OV. Capistrano MF.Yuldasheva LN. Nogueira RA. Influence of Cys-130 S. aureus alpha-toxin on planar lipid bilayer and erythrocyte membranes. The Journal of membrane biology.1997; 156(2), 157-172:
17.    Zhang D. Palmer J.Teh KH. Calinisan MM Flint S. Milk fat influences proteolytic enzyme activity of dairy Pseudomonas species. International journal of food microbiology. 2020; 320, 108543:
18.    Rosini R. Margarit I. Biofilm formation by Streptococcus agalactiae: influence of environmental conditions and implicated virulence factors. Frontiers in cellular and infection microbiology. 2015; 5: 6:
19.    Iseppi R. Tardugno R. Brighenti V. Benvenuti S. Sabia C. Pellati F. Messi P. Phytochemical Composition and In Vitro Antimicrobial Activity of Essential Oils from the Lamiaceae Family against Streptococcus agalactiae and Candida albicans Biofilms. Antibiotics. 2020; 9(9), 592:
20.    López Y. Parra E. Cepas V. Sanfeliú I. Juncosa T. Andreu A. Soto SM. Serotype, virulence profile, antimicrobial resistance and macrolide-resistance determinants in Streptococcus agalactiae isolates in pregnant women and neonates in Catalonia, Spain.  Enfermedades infecciosas y microbiologia clinica.2018; 36(8), 472-477:
21.    Zheng JX. Chen Z. Xu ZC. Chen JW. Xu GJ. Sun X. Qu D. In vitro evaluation of the antibacterial activities of radezolid and linezolid for Streptococcus agalactiae. Microbial pathogenesis. 2020; 139, 103866.‏
22.    Smani Y. McConnell MJ. Pachón J. Role of fibronectin in the adhesion of Acinetobacter baumannii to host cells. PLoS One. 2012; 7(4):
23.    Salman SA. Aldeen WR. Antibacterial, Anti-virulence factors of Hibiscus sabdariffa extracts in Staphylococcus aureus isolated from patients with urinary tract infection. Research Journal of Pharmacy and Technology. 2018; 11(2), 735-740: doi : 10.5958/0974-360X.2018.00138.5
24.    Yang CH. Su PW. Moi SH. Chuang LY. Biofilm formation in Acinetobacter baumannii:  genotype-phenotype correlation. Molecules. 2019; 24(10), 1849:
25.    Reddy GS. Srinivasulu K. Mahendran B. Reddy RS. Biochemical characterization of anti-microbial activity and purification of glycolipids produced by dodecanoic acid-undecyl ester. Research Journal of Pharmacy and Technology. 2018; 11(9), 4066-4073: doi : 10.5958/0974-360X.2018.00748.5
26.    Stowe  SD. Richards JJ. Tucker AT. Thompson R. Melander C. Cavanagh J. Anti-biofilm compounds derived from marine sponges. Marine Drugs. 2011; 9(10), 2010-2035:
27.    Mohammed GJ. Abdul-Razaq MS. Grouping and Revelation the significant Virulence genes of Escherichia coli isolated from Patients with Urinary Tract Infections. Research Journal of Pharmacy and Technology. 2018; 11(12), 5483-5489: doi : 10.5958/0974-360X.2018.00999.X
28.    Mussa A. Ziayt M. Study the effect of Purified Pyoluteorin Produced from P. aeruginosa, Isolated from Rhizospheric Plant Wheat on some UTI Bacteria Biofilm Formation. Research Journal of Pharmacy and Technology. 2018; 11(12), 5529:‏ doi : 10.5958/0974-360X.2018.01006.5
29.    Ju J. Xie Y. Yu H. Guo Y. Cheng Y. Qian H. Yao W.  Synergistic interactions of plant essential oils with antimicrobial agents: a new antimicrobial therapy. Critical Reviews in Food Science and Nutrition. 2020; 1-12:

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