Author(s): P. Manorama, Valmiki Aruna, Gangadhara Angajala, D. Geetha


DOI: 10.52711/0974-360X.2022.00605   

Address: P. Manorama1, Valmiki Aruna1, Gangadhara Angajala1,2*, D. Geetha1
1Department of Chemistry, Kalasalingam Academy of Research and Education, Anand Nagar, Krishnankoil - 626126, Tamilnadu, India.
2Department of Humanities and Sciences, Vallurupalli Nageswara Rao Vignana Jyothi Institute of Engineering and Technology, Hyderabad, 500090, Telangana, India.
*Corresponding Author

Published In:   Volume - 15,      Issue - 8,     Year - 2022

The present study attempts to investigate the antioxidant activity and chromatographic profiling using HPLC and GC-MS technique of selected Indian Medicinal herbs. Evaluation of antioxidant activity of the methanolic extract of whole plant of Marsilea-quadrifolia (Sample 1), whole plant of Trigonella-foenum-graecum (sample 2), flowers of Cassia-auriculata (Sample 3), leaves of Mangifera-indica (sample 4), flower of Musa-paradisiaca (Sample 5), seeds of Eugenia-jambolana (sample 6), leaves of Aegle-marmelos (sample 7) and poly herbal Extract (PHE) with equal ratio of the mixture of the seven plants powder (Sample 8) were investigated by DPPH (2,2-diphenyl-1-picryl-hydrazyl) assay, Hydrogen peroxide assay and Nitric oxide scavenging activity. Percentage of inhibition and half-inhibition concentration, IC50 values were reported. GC-MS and HPLC analysis of PHE revealed the presence of various Chemical compounds which mainly includes polyphenols and flavanoids responsible for antioxidant activity.

Cite this article:
P. Manorama, Valmiki Aruna, Gangadhara Angajala, D. Geetha. Identification of Chemical Constituents, Chromatographic profiling of PHE (Poly Herbal Extract) of selected Indian Medicinal Herbs and its Antioxidant activity Evaluation. Research Journal of Pharmacy and Technology. 2022; 15(8):3611-7. doi: 10.52711/0974-360X.2022.00605

P. Manorama, Valmiki Aruna, Gangadhara Angajala, D. Geetha. Identification of Chemical Constituents, Chromatographic profiling of PHE (Poly Herbal Extract) of selected Indian Medicinal Herbs and its Antioxidant activity Evaluation. Research Journal of Pharmacy and Technology. 2022; 15(8):3611-7. doi: 10.52711/0974-360X.2022.00605   Available on:

1.    David OK. Emma LW. Herbal Extracts and Phytochemicals: Plant Secondary Metabolites and the Enhancement of Human Brain Function. Adv Nutr. Jan 10 2011; 2(1):32-50.
2.    World Health Organization (WHO), National Policy on Traditional Medicine and Regulation of Herbal Medicines. Geneva: Report of WHO global survey 2005.
3.    Angajala G. Pavan Kumar P. Subashini R. One-step biofabrication of copper nanoparticles from Aegle marmelos correa aqueous leaf extract and evaluation of its anti-inflammatory and mosquito larvicidal efficacy. RSC Adv. Oct 15 2014; 4:51459-51470.
4.    Praveen Patidar. Kamlesh Dashora. Spectrophotometric Estimation of Total Polyphenols as Tannic Acid in Ayurvedic Formulation Astangavaleha. Asian J. Pharm. Ana. Jan.-Mar. 2014; 4(1):42-44.
5.    Angajala G. Subashini R. Evaluation of larvicidal potential of β-sitosterol isolated from indigenous Aegle marmelos Correa crude leaf extracts against blood feeding parasites and its binding affinity studies towards sterol carrier protein. Biocatalysis & Agricultural Biotechnology. 2018 Oct 13; 16:586-593.
6.    Krishna Kondragunta V. Karuppuraj V. Perumal K. Antioxidant activity and Folic acid content in indigenous isolates of Ganoderma lucidum. Asian J. Pharm. Ana. 2016; 6(4):213-215.
7.    Perrin L. Rakik A. Yearly S. Baumbeger C. Kinloch-de Loies S. Pechiere M. Hirschel B. Combined therapy with zidovudine and L-697,661 in primary HIV infection. AIDS. 1996 Sep; 10(11):1233-1237.
8.    Muthusamy Senthil Kumar. Srinivasan Balachandran. Shibani Chaudhury. Influence of Incubation Temperatures on Total Phenolic, Flavonoids Content and Free Radical Scavenging Activity of Callus from Heliotropium indicum L. Asian J. Pharm. Res. Oct.-Dec. 2012; 2(4):148-152.
9.    Valli G. Jeyalakshmi M. Preliminary Phytochemical and Antioxidant Study of Odina woodier Leaf Extract. Asian J. Pharm. Res. Oct.-Dec. 2012; 2(4):153-155.
10.    Uttpal A. Jacobo-Herrera N. Altemimi A. Naoufal L. A Comprehensive Review on Medicinal Plants as Antimicrobial Therapeutics: Potential Avenues of Biocompatible Drug Discovery. Metabolites. Nov 1; 9:258. 10.3390/metabo9110258
11.    Kinghorn AD. Soejarto DD. Nanayakkara NP. Compadre CM. Makapugay HC. Hovanec-Brown JM. Medon PJ. Kamath SK. A phytochemical screening procedure for sweet ent-kaurene glycosides in the genus Stevia. J. Nat. Prod. June 1989; 47(3):439-444.
12.    Malathi R. Ahamed John S. Cholarajan A. Antioxidant Activity of Extract from the Leaves of Tylophora asthmatica. Asian J. Res. Pharm. Sci. April-June 2012; 2(2):80-82.
13.    Madhu CH. Swapna J. Neelima Monic K. Shah V. A Comparative Evaluation of the Antioxidant Activity of Some Medicinal Plants Popularly Used in India. Asian J. Res. Pharm. Sci. July-Sept. 2012; 2(3):98-100.
14.    Senguttuvan J. Paulsamy S. Karthika K. Phytochemical analysis and evaluation of leaf and root parts of the medicinal herb, Hypochaeris radicata L. for in vitro antioxidant activities. Asian Pac J Trop Biomed. May 2014; 4:S359-S67.
15.    Nobuji N. Shin-ichi K. Hiroe K. Keiko S. Kiyoshi K. Takahiko M. Identification, Quantitative Determination, and Antioxidative Activities of Chlorogenic Acid Isomers in Prune (Prunus domestica L.). J Agric Food Chem. Oct 6 2000; 48:5512-5516.
16.    Pandey KB. Rizvi SI. Plant polyphenols as dietary antioxidants in human health and disease Oxid Med Cell Longev. Dec 2009; 2(5):270-278.
17.    Wilson DW. Nash P. Buttar HS. Griffiths K. Singh R. Meester FD. Horiuchi R. Takahashi T. The Role of Food Antioxidants, Benefits of Functional Foods, and Influence of Feeding Habits on the Health of the Older Person: An Overview. Antioxidants (Basel). Oct 28 2017; 6(4):81.
18.    Mrunali Potbhare. Deepak Khobragade. In Vitro Evaluation of Antioxidant Potential of Ayurvedic Preparations Lauha Bhasma and Mandura Bhasma. Asian J. Pharm. Res. 2017; 7(2):63-66.
19.    Amit Roy. Dayananda Bhoumik. Ram Kumar Sahu. Jaya Dwivedi. Phytochemical Screening and Antioxidant Activity of Sesbania grandiflora Leaves Extracts. Asian J. Res. Pharm. Sci. Jan.-Mar. 2014; 4(1):16-21.
20.    Kruk J. Enein HYA. Kładna A. Bowser JE. Psychological Stress and Cellular Aging in Cancer: A Meta-Analysis. Free Radical Research. Apr 17 2019; 53(5):497-521.
21.    Nita M. Grzybowski A. The Role of the Reactive Oxygen Species and Oxidative Stress in the Pathomechanism of the Age-Related Ocular Diseases and Other Pathologies of the Anterior and Posterior Eye Segments in Adults. Oxid Med Cell Longev. Jan 10 2016; 3164734.
22.    Phaniendra A. Jestadi DB. Periyasamy L. Free radicals: properties, sources, targets, and their implication in various diseases. Indian J Clin Biochem. Jan 2015; 30:11-26.
23.    Kohen R. Nyska A. Oxidation of biological systems: oxidative stress phenomena, antioxidants, redox reactions, and methods for their quantification. Toxicol Pathol. Dec 2002; 30(6):620-650.
24.    Halliwell B, Free Radicals and other reactive species in disease. Nature Encyclopedia of life sciences. Mar 16 2001; 1.
25.    Genestra M. Oxyl radicals, redox-sensitive signalling cascades and antioxidants. Review Cell Signal. Cell Signal. Sep 2007; 19(9):1807-1819.
26.    Kurutas EB. The importance of antioxidants which play the role in cellular response against oxidative/nitrosative stress: current state. Nutr J. July 2016; 15(1):71.
27.    Pham-Huy LA. Hua H. Pham-Huy C. Free Radicals, Antioxidants in Disease and Health. Int J Biomed Sci. June 2008; 4(2):89-96.
28.    Kasote DM. Katyare SS. Hegde MV. Bae H. Significance of antioxidant potential of plants and its relevance to therapeutic applications. Int J Biol Sci. June 11 2015; 11(8):982-991.
29.    Uttara B. Singh AV. Zamboni P. Mahajan RT. Oxidative stress and neurodegenerative diseases. A Review of upstream and downstream antioxidant therapeutic options. Curr Neuropharmacol. Indian J Clin Biochem. Mar 2009; 7(1):65-74.
30.    Mahinda W. Fereidoon S. Antioxidant and free radical-scavenging properties of ethanolic extracts of defatted borage (Borago officinalis L.) seeds. Food Chem. Dec 1999; 67(4):399-414.
31.    Duh PD. Yen GC. Yen WJ. Chang LW. Antioxidant effects of water extracts from barley (Hordeum vulgare L.) prepared under different roasting temperatures. J. Agric. Food Chem. Mar 2001; 49(3):1455-1463.
32.    Evans CR. Burden R. Free radical-lipid interactions and their pathological consequences. Prog Lipid Res. Jan 1993; 32(1):71-110.
33.    Sies H. Antioxidants in Disease. Mechanisms and Therapy. Academic Press. New York. 1996.
34.    Oyaizu M. Studies on products of browning reaction-antioxidative activities of products of browning reaction prepared from glucosamine. Jpn.J.Nutr.Diet. 1986; 44(6):307-315.
35.    Zar JH. Biostatistical Analysis. Upper Saddle River. NJ: Prentice Hall; 1999.

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