Author(s):
Vikas Verma Patel, Saurabh Kumar, Nagendra Prasad
Email(s):
vikasbotany@gmail.com
DOI:
10.52711/0974-360X.2023.00820
Address:
Vikas Verma Patel1*, Saurabh Kumar2, Nagendra Prasad3
1Assistant Professor, Department of Botany, V.R.A.L. Government Girls Degree College, Bareilly- 243001. M.J.P. Rohilkhand University, Bareilly-243001, U.P., India.
2Assistant Professor, Department of Botany, D.A.V. (PG) College, Muzaffarnagar, U.P., India- 251001.
3Birbal Sahni Institute of Palaeosciences, 53-University Road, Lucknow-226007, U.P., India.
*Corresponding Author
Published In:
Volume - 16,
Issue - 11,
Year - 2023
ABSTRACT:
Aflatoxin, a mycotoxin found commonly in maize, peanuts, and sunflower worldwide, is associated with liver cancer, acute toxicosis, and growth impairment in humans and animals. In India, sunflower seeds are a source of snacks, cooking oil, and animal feed. These seeds are a potential source of aflatoxin contamination. However, reports on aflatoxin contamination in sunflower seeds and cakes are scarce. The objective of the current study was to determine the potential of Aspergillus flavus and total aflatoxin concentrations in sunflower seeds and cakes from small-scale oil processors in Rohilkhand region of Uttar Pradesh. 126 cultures of Aspergillus flavus were obtained from sunflower kernels, which showed wide variation in their cultural characters. Colour of conidial heads being a stable character was used to divide them into four groups out of which two representative isolates each, based on minimum and maximum number of sclerotia cm-2 of the culture medium were selected. Aflatoxin production by an isolate had positive correlation with the sclerotia production. These results can help in identifying the potent of A. flavus isolates for aflatoxin production and developing proper management strategies. In summary, humans and animals are potentially at high risk of exposure to aflatoxins through sunflower seeds and cakes from micro-scale millers in India and location influences risk.
Cite this article:
Vikas Verma Patel, Saurabh Kumar, Nagendra Prasad. Exploration of aflatoxigenic potential and seed colonization of Aspergillus flavus in sunflower. Research Journal of Pharmacy and Technology. 2023; 16(11):5063-6. doi: 10.52711/0974-360X.2023.00820
Cite(Electronic):
Vikas Verma Patel, Saurabh Kumar, Nagendra Prasad. Exploration of aflatoxigenic potential and seed colonization of Aspergillus flavus in sunflower. Research Journal of Pharmacy and Technology. 2023; 16(11):5063-6. doi: 10.52711/0974-360X.2023.00820 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2023-16-11-14
REFERENCES:
1. Asao T. G. Buchi M.M. Abdelkader S.B. Chang E.L. Wick and G.N. Wagan. Aflatoxin B and G. J. Amer. Chem. Soc.1965; 85:1706-1707. https://doi.org/10.1021/ja01082a031
2. Hesseltine C.W. O.L. Shotwell J.J. Ellis R.D. Stubblefield. Aflatoxin formation by Aspergillus flavus. Bact. Rev. 1966; 30: 798-805. https://doi.org/10.1128/br.30.4.795-805.1966
3. Nagarajan V. Bhat R.V. and Tulpule P.G. Aflatoxin production in Sunflower (Helianthus annuus) seed varieties. Current Science. 1974; 43: 603-604. https://link.springer.com/article/10.1007/BF01935130
4. Mehan V.K. and J.S Chohan. Aflatoxin B1 producing potential of isolates of Aspergillus flavus from cotton, maize and wheat. Mycopath. Mycol. Appl. 1973; 49(4): 263-274. https://doi.org/10.1007/BF02050720
5. Banu N. Muthumary J.P. Screening of aflatoxigenic property of some Aspergillus flavus isolated from sunflower seeds and its products at sunflower oil refineries. Research J. Science and Tech. 2010; 2(5): 102-107. https://www.researchgate.net/publication/263655530
6. Bedi J.S. Evaluation of different methods of assay of aflatoxins produced by different isolates of Aspergillus flavus link ex. Fr. M.Sc. Thesis, Punjab Agricultural University. Ludhiana pp.1978; 62.
7. Mangala U.N. Reddy KRN Singotamu L. Chary PMS Reddy C.S. and Muralidharan K. Aspergilli colonize and produce Aflatoxin B1 in Dissolved Rice grains .J. Mycol. Pl. Pathol. 2006; 36(3): 418-426. https://www.researchgate.net/publication/200053784
8. Miller J.D. Fungi and mycotoxins in grain implications for stored product research. Journal of Stored Product Research. 1995; 31(1): 1-16. https://doi.org/10.1016/0022-474X(94)00039-V
9. Galvano F. Piva A. Ritieni A. and Galvano G. Dietary strategies to counteract the effect of mycotoxins: A review. Journal of Food Protection. 2001; 64(1): 120-131. https://doi.org/10.4315/0362-028x-64.1.120
10. Mohana D. C. Thippeswamy S. Abhishek R. U. Shobha B. and Mamatha M. G. Studies on seed-borne mycoflora and aflatoxin B1 contaminations in food based seed samples: Molecular detection of mycotoxigenic Aspergillus flavus and their management. International Food Research Journal. 2016; 23(6): 2689-2694. http://www.ifrj.upm.edu.my
11. Zhang J.D. Han L. Yan S. and Liu C.M. The non-metabolizable glucose analog D-glucal inhibits aflatoxin biosynthesis and promotes kojic acid production in Aspergillus flavus. BMC Microbiology. 2014; 14: 95. https://bmcmicrobiol.biomedcentral.com/articles/10.1186/1471-2180-14-95
12. Meena A.K. Singh U. Singh A. Mishra S. Rao M.M. Padhi M.M. Babu R. Gaurav A. A Comparative Study of the Quality Assessment of Vitex negundo Linn Leaves Collected from Three Different Geographical Locations. Asian J. Research Chem. 2011; 4(6): 986-989. https://www.researchgate.net/publication/264708195
13. Suryanarayanan T.S. Suryanarayanan C.S. Fungi associated with stored sunflower seeds. Journal of Economic and Taxonomic Botany. 1990; 14: 174-176. https://www.semanticscholar.org/paper
14. Jimenez M. Mateo R. Querol A. Huerta T. and Hernanez E. Mycotoxins and mycotoxigenic moulds in nuts and sunflower seeds for human consumption. Mycopathologia. 1991. 115: 121-127. https://link.springer.com/article/10.1007/BF00436800
15. Davis N.D. Diener Y.L. and D.W. Eldridge. Production of aflatoxin Band G by Aspergillus flavus in a semi – synthetic medium. Appl. Microbiol. 1966; 14: 378-380. https://pubmed.ncbi.nlm.nih.gov/5970823/
16. Pons W.A. Jr. A.F. Cucullu A.S. Lee J.A. Robertson A.O. Franz and L.A. Goldblatt. Determination of aflatoxins in agricultural products: Use of aqueous acetone for extraction. J.A.O.A.C. 2020; 49:554. https://doi.org/10.1093/jaoac/49.3.554
17. Garber R.K and P.J. Cotty. Formation of sclerotia and aflatoxins in developing cotton bolls infected by S strain of Aspergillus flavus and potential for biocontrol with atoxigenic strain. Phytophathology. 1997; 87(9): 940-945. http://dx.doi.org/10.1094/PHYTO.1997.87.9.940
18. Passone M.A. Resnik S. and Etcheverry M.G. The potential of food grade antioxidants in the control of Aspergillus section flavi, interrelated mycoflora and aflatoxin B1 accumulation on peanut grains. Food Control. 2008; 19: 364-371. https://doi.org/10.1016/j.foodcont.2007.04.014
19. M. Gabriella, C. Angeli, A. Ambrus , A. Nagy, V. Kardos , A. Zentai , K. Kerekes , Z. Farkas , A. Jozwiak and T. Bartok. Detection of Aflatoxins in Different Matrices and Food-Chain Positions. Frontiers in Microbiology 2020; 11: 1916. doi: https://doi.org/10.3389/fmicb.2020.01916