Author(s):
Venna R Surya Anusha, Koppala RVS Chaitanya, Rajeswari Aleti, Kolapalli Venkata Ramana Murthy
Email(s):
rajeswarianusha@gmail.com
DOI:
10.52711/0974-360X.2023.00844 
Address:
Venna R Surya Anusha1*, Koppala RVS Chaitanya2, Rajeswari Aleti1, Kolapalli Venkata Ramana Murthy1
1AU College of Pharmaceutical Sciences, Andhra University, Visakhapatnam, Andhra Pradesh, India, 530003.
2Department of Pharmacology, Vignan Institute of Pharmaceutical Technology, Kapujaggrajupeta, Duvvada, Visakhapatnam, India, 530049.
*Corresponding Author
Published In:
Volume - 16,
Issue - 11,
Year - 2023
ABSTRACT:
A simple, reliable and sensitive RP-HPLC bioanalytical method with PDA as detector was developed for the estimation of donepezil HCl in the rabbit plasma and validated. The biological samples were extracted and analysed using loratadine as internal standard. The sample analysed with Inertsil ODS 150mm x 4.6mm, 5.0µm particle size column using 70:30 v/v of 0.1% trifluoroacetic acid and acetonitrile mobile phase at flow rate of 1mL/min. All the parameters validated were within the limits proposed in bioanalytical method validation guidelines. The linearity was observed in the range of 5-200ng/mL with LOD of 0.9ng/mL. The method was observed to have short analysis time of 6 min and also found to be successful in studying the pharmacokinetic parameters of donepezil HCl.
Cite this article:
Venna R Surya Anusha, Koppala RVS Chaitanya, Rajeswari Aleti, Kolapalli Venkata Ramana Murthy. Bio-analytical Method for the Pre-clinical Estimation of Donepezil Hydrochloride in Rabbit Plasma by RP-HPLC. Research Journal of Pharmacy and Technology. 2023; 16(11):5207-2. doi: 10.52711/0974-360X.2023.00844
Cite(Electronic):
Venna R Surya Anusha, Koppala RVS Chaitanya, Rajeswari Aleti, Kolapalli Venkata Ramana Murthy. Bio-analytical Method for the Pre-clinical Estimation of Donepezil Hydrochloride in Rabbit Plasma by RP-HPLC. Research Journal of Pharmacy and Technology. 2023; 16(11):5207-2. doi: 10.52711/0974-360X.2023.00844 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2023-16-11-38
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