Aluminium oxide (Al2O3) nanoparticles (NPs) were formed via laser ablation of an aluminium target in deionised water (DIW) (Nd: YAG laser; wavelength: 1,064nm; different laser energies: 500, 800 and 1000 mJ; 30min). The optical, structural and morphological features of these Al2O3 NPs were investigated via ultraviolet/visible (UV/Vis) spectroscopy, scanning electron microscopy; X-ray diffraction (XRD) analysis, transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy. Show that the average size of nanoparticles was between (21-48nm). The antibacterial activities of Al2O3 NPs were produced by utilising the well diffusion method against two pathogens (Pseudomonas aeruginosa and Bacillus cereus). Al2O3 NPs demonstrated significant antibacterial activity against P. aeruginosa and B. cereus compared with the control (P=0.05). Al2O3 NPs had the best energy at 1000 mJ, indicating that they were more effective towards Gram +ve than Gram -ve bacteria. The synergistic/antibacterial activity of Al2O3 NPs exhibited potential antibacterial activity against the investigated species after being combined with imipenem and gentamicin, which had higher antibacterial action than Al2O3 NPs alone. Furthermore, as determined by DPPH, results suggested that Al2O3 NPs have antioxidant properties. Finally, Al2O3 NPs were tested for cytotoxicity against the breast cancer cell line (MCF-7), where 500mJ was 62.33±2.33, 800 mJ was 73.00±2.082 and 1000mJ was 85.00 ±1.732. The last was more effective than 500 mJ and 800 mJ and more efficient in penetrating cell membrane.
Cite this article:
Tuqa Sabah, Kareem H. Jawad, Nebras Al-attar. Synthesis and Biomedical Activity of Aluminium Oxide Nanoparticles by Laser Ablation Technique. Research Journal of Pharmacy and Technology 2023; 16(3):1267-3. doi: 10.52711/0974-360X.2023.00209
Tuqa Sabah, Kareem H. Jawad, Nebras Al-attar. Synthesis and Biomedical Activity of Aluminium Oxide Nanoparticles by Laser Ablation Technique. Research Journal of Pharmacy and Technology 2023; 16(3):1267-3. doi: 10.52711/0974-360X.2023.00209 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2023-16-3-45
1. Rane AV, Kanny K, Abitha VK, Thomas S. Methods for synthesis of nanoparticles and fabrication of nanocomposites. Synthesis of inorganic nanomaterials, Elsevier, 2018; 2018: 121–139. https://doi.org/10.1016/B978-0-08-101975-7.00005-1.
2. Kato H. Tracking nanoparticles inside cells. Nature Nanotechnology, 2011; 6(3): 139–140.
3. Logothetidis S. Nanotechnology: Principles and applications,” in Nanostructured materials and their applications, Springer. 2012. PP 1-22. ISBN 9789814877435
4. Kunjwani HK, Manikrao AM, Rajesh KS, Sable VP, NH Indurwade. Natural Gums as Matrix and Coating Material for Colon Specific Drug Delivery. Research Journal of. Pharmacy and Technology. 2009; 2(4): 705-709.
5. Jangde RK, Sulekha Khute RS. Design and Development of Ciprofloxacin Lipid Polymer Hybrid Nanoparticle by Response Surface Methodology. Research Journal of Pharmacy and Technology. 2020; 13(7): 3249-3256. DOI: 10.5958/0974-360X.2020.00576.4
6. Brannon-Peppas L, Blanchette JO. Nanoparticle and targeted systems for cancer therapy. Advances in Drug Deliveviry Reviews. 2004; 56(11): 1649–1659.
7. Chatterjee S, Gupta S, Mukherjee S, MukherjeeJ. Nanomaterial Mediated Drug Delivery, Image-Guided Therapy and Multifaceted Theranostic Systems in Cancer. Journal of Nanotechnology and Advanced Matererials. 2014; 2(2): 77–88.
8. Arti S. Synthesis, Characterization and Analytical Applications of Antimony (III) Tungstophosphatean Inorganic Ion Exchange Material. Asian Journal Research Chemistry. 2012; 5(10): 1281-1288.
9. Bala T, Armstrong G, Laffir F, Thornton R. Titania–silver and alumina–silver composite nanoparticles: novel, versatile synthesis, reaction mechanism and potential antimicrobial application. Journal of Colloid Interface Sciences. 2011; 356(2): 395–403.
10. Mukherjee A, Sadiq IM, Prathna TC, Chandrasekaran N. Antimicrobial activity of aluminium oxide nanoparticles for potential clinical applications. Science against Microbial Pathogense Communicating Current Research and Technological Advances. 2011; 1: 245–251.
11. Neddersen J, Chumanov G, Cotton TM. Laser ablation of metals: a new method for preparing SERS active colloids. Applied Spectroscopy. 1993; 47(12): 1959–1964.
12. Gaumet M, Vargas A, Gurny R, Delie F. Nanoparticles for drug delivery: the need for precision in reporting particle size parameters. European Journal of Pharmaceutics and Biopharmaceutics. 2008; 69(1): 1–9.
13. Nikalje AP. Nanotechnology and its applications in medicine. Medicinal Chemistry. 2015; 5(2): 81–89.
14. Ansari MA, Khan HM, Khan AA, Sultan A, Azam A. Characterization of clinical strains of MSSA, MRSA and MRSE isolated from skin and soft tissue infections and the antibacterial activity of ZnO nanoparticles. World Journal of Microbiology and Biotechnology. 2012; 28(4): 1605–1613. doi: 10.1007/s11274-011-0966-1.
15. Ananthalakshmi R, Rajarathinam SRX, Sadiq AM. Antioxidant activity of ZnO Nanoparticles synthesized using Luffa acutangula peel extract. Research Journal of Pharmacy and Technology. 2019; 12(4):1569-1572. DOI: 10.5958/0974-360X.2019.00260.9
16. Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA, Jemal A. Erratum: Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries,” Ca Cancer Journal for Clinicians. 2020; 70(4): 313.
17. Chen W, Qin M, Chen X, Wang Q, Zhang Z, Sun X. Combining photothermal therapy and immunotherapy against melanoma by polydopamine-coated Al2O3 nanoparticles. Theranostics. 2018; 8(8): 2229.
18. Mohanty S, Panda S, Purohit D, Chandra S. A Comprehensive Review on PLGA - based Nanoparticles used for Rheumatoid Arthritis. Research Journal of Pharmacy and Technology. 2019; 12(3): 1481-1488
19. Devi GL, Suruthi P, Veerakumar RV, Vinoth S, Subbaiya R, Chozhavendhan S. A Review on Metallic Gold and Silver Nanoparticles. Research Journal of Pharmacy and Technology. 2019; 12(2): 935-943. DOI: 10.5958/0974-360X.2019.00158.6
20. Asha S, Thirunavukkarasu P, Rajeshkumar S. Green Synthesis of Silver Nanoparticles using Mirabilis jalapa Aqueous Extract and their Antibacterial Activity against Respective Microorganisms. Research Journal of Pharmacy and Technology. 2017; 10(3): 811-817. DOI: 10.5958/0974-360X.2017.00153.6
21. Inbakani SA, Siva R. Biosynthesis of Silver Nanoparticles using Edible Mushrooms and It’s bactericidal Activities. Research Journal of Pharmacy and Technology. 2017; 10(2): 467-472. DOI:10.5958/0974-360X.2017.00094.4
22. Maeh RK, Jaafar AI, Hasoon BAAL, Hussein NN. Preparation and characterization of graphene oxide for biological application. Drug Invention Today/ 2020; 14: 5. DOI: 10.13140/RG.2.2.23987.86562
23. Hasoon BA, Abdulwahab AI, Maeah R, Al-azaw KF. Preparation and Characterization of Silver Nanoparticle by Cordia myxa Extract and their Study Anticancer, Antioxidant, Antibacterial Activity. Indian Journal of Forensic Medicine & Toxicology. 2021; 15: 3. DOI: https://doi.org/10.37506/ijfmt.v15i3.16625
24. Maeah RK, Hasoon B.A, Abd-Alwahab AI, Al- Azawi KF, Hameedi WB. Biosynthesis of silver nanoparticles using Hibiscus sabdariffa and their biological application. EurAsian Journal of Biosciences. 2020; 14(2): 3377-3383.
25. Jawad KH. Effects of Ag nanoparticles prepared by Nd-YAG lasers and study their antibacterial and antioxidant activity. Plant Archives. 2020; 20(Suppl1): 3481-3486.
26. Baladi A, Mamoory RS. Effect of laser wavelength and ablation time on pulsed laser ablation synthesis of Al nanoparticles in ethanol. International Journal of Modern Physics. Conference Series, 2012, 5: 58–65.
27. Jabir MS, Nayef UM, Jawad KU, Taqi ZJ, Hasoon BA, Nada R. Ahmed3. Porous silicon nanoparticles prepared via an improved method: a developing strategy for a successful antimicrobial agent against Escherichia coli and Staphylococcus aureus. IOP Conf. Series: Materials Science and Engineering. 2018; 454: 012077. doi:10.1088/1757-899X/454/1/012077
28. Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing, 29th ed CLSI document M100. Clinical and Laboratory Standards Institute, Wayne, PA. 2019.
29. Hochbaum AI, Kolodkin-Gal I, Foulston L, Kolter R, Aizenberg J, Losick, R. Inhibitory effects of D-amino acids on Staphylococcus aureus biofilm development. Journal of bacteriology. 2011; 193(20): 5616-5622.
30. Hasson BA, Mahdi LH, Essa RH. Evidence of Antioxidant Activity of Novel L-Glutaminase Purified from L. Gasseri BRLHM. Journal of Applied Sciences and Nanotechnology. 2021; 4: 2021. DOI: 10.53293/jasn.2021.3969.1064.
31. Jawad KH. Hasson BA. Developing Strategy for a Successful Antioxidant, Anticancer Activity via an Improved Method Prepared to Porous Silicon Nanoparticles. ournal of Applied Sciences and Nanotechnology. 2021: 1(4): 1-11. DOI: 10.53293/jasn.2021.3890.105.
32. Al-Ziaydi AG, Hamzah MI, Al-Shammari AM, Kadhim HS, Jabir MS. The anti-proliferative activity of D-mannoheptulose against breast cancer cell line through glycolysis inhibition. AIP Conference Proceedings. 2020; 2307(1): 20023.
33. Al-Ziaydi AG, Al-Shammari AM, Hamzah MI, KadhimHS, Jabir MS. Newcastle disease virus suppress glycolysis pathway and induce breast cancer cells death. Virusdisease. 2020; 31(3): 341–348.
34. Jabir MS, et al. Fe3O4 nanoparticles capped with PEG induce apoptosis in breast cancer AMJ13 cells via mitochondrial damage and reduction of NF-κB translocation,” Journal of Inorganic and Organometallic Polymer Materials. 2021; 31(3): 1241–1259.
35. Jabir MS, et al. Green synthesis of silver nanoparticles from Eriobotrya japonica extract: a promising approach against cancer cells proliferation, inflammation, allergic disorders and phagocytosis induction. Artif. cells, nanomedicine, Biotechnology. 2021; 49(1): 48–60.
36. Khashan KS, Abdulameer FA, Jabir MS, Hadi AA, Sulaiman GM. Anticancer activity and toxicity of carbon nanoparticles produced by pulsed laser ablation of graphite in water. Advances in Nature Science, Nanoscience, and Nanotechnology. 2020; 11(3): 35010.
37. Bahjat hh, Ismail RA, Sulaiman GM, Jabir MS. Magnetic field-assisted laser ablation of titanium dioxide nanoparticles in water for anti-bacterial applications. Journal of Inorganic Organometallic Polymer Materials. 2021; 31(9): 3649–3656.
38. Lau Truong S, Levi G, Bozon-Verduraz F, Petrovskaya AV, Simakin AV, Shafeev GA. Generation of Ag nanospikes via laser ablation in liquid environment and their activity in SERS of organic molecules. Applied Physics. 2007; 89(2): 373–376.
39. Eustis S. Gold and silver nanoparticles: characterization of their interesting optical properties and the mechanism of their photochemical formation. Georgia Institute of technology, 2006. https://smartech.gatech.edu/handle/1853/16226
40. Yan Z, Bao R, Huang Y, Chrisey DB. Hollow particles formed on laser-induced bubbles by excimer laser ablation of Al in liquid. Journal of Physical Chemistry C. 2010; 114(26): 11370–11374.
41. Liu IL, Shen P, Chen SY. H+- and Al2+-codoped Al2O3 nanoparticles with spinel-type related structures by pulsed laser ablation in water. Journal of Physical Chemistry C. 2010; 114(17): 7751–7757.
42. Kumar B, Thareja RK. Synthesis of nanoparticles in laser ablation of aluminum in liquid. Journal of Applied Physics. 2010; 108(6): Article ID 064906.
43. Chwalibog A, et al.Visualization of interaction between inorganic nanoparticles and bacteria or fungi. International Journal of Nanomedicine. 2010; 5: 1085. doi: 10.2147/IJN.S13532
44. Yan Z, Bao R, Huang Y, Chrisey DB. Hollow particles formed on laser-induced bubbles by excimer laser ablation of Al in liquid,” Journal of Physical Chemistry. 2010; 114(26): 11370–11374.
45. Lesniak A, Salvati A, Santos-Martinez MJ, Radomski MW, Dawson KA, ÅbergC. Nanoparticle adhesion to the cell membrane and its effect on nanoparticle uptake efficiency. Journal of American Chemical Society. 2013; 135(4): 1438–1444.
46. Sarwar A, Katas H, Samsudin SN, Zin NM. Regioselective sequential modification of chitosan via azide-alkyne click reaction: synthesis, characterization, and antimicrobial activity of chitosan derivatives and nanoparticles. PLoS One. 2015; 10(4): e0123084.
47. Hasan DMA, Hasoon BA, Abdulwahab A, Jawad KH. Biological activities of Ethanolic Extract Produced by Cucurbita pepo plant. Revista Bionatura. 2022; 7(2): 19. http://dx.doi.org/10.21931/RB/2022.07.02.19.
48. Talaeiab AJ, Zareic N, Hasande A, Bloukhf SH, et al. Fabrication of inorganic alumina particles at nanoscale by a pulsed laser ablation technique in liquid and exploring their protein binding, anticancer and antipathogenic activities. Arabian Journal of Chemistry. 2021; 14(2): 102923. https://doi.org/10.1016/j.arabjc.2020.102923
49. Jawad AS, Thewaini QN, Al-Musawi S. Cytotoxicity Effect and Antibacterial Activity of Al2O3 Nanoparticles Activity against Streptococcus Pyogenes and Proteus Vulgaris. Journal of Applied Sciences and Nanotechnology. 2021; 1(3): 42-50. DOI: 10.53293/jasn.2021.3944.1061
50. Shrivastava R, Bhargava R, Flora SJS. Antioxidant activity and free radical scavenging potentialof alpha lipoic acid and quercetin against Al2O3 nanoparticle-induced toxicity in mice. Free Radicals and Antioxidants. 2014; 4: 1