Author(s):
Aqsa Fathima, Shivaprakash Gangachannaiah, Ujjal Bose, Shama Prasada Kabekkodu, Rituparna Chakraborty, Praveen Kumar S E, Padmanabha Udupa, Rachagolla Sai Prathap Yadav, Vidya Monappa
Email(s):
shiva.g@manipal.edu
DOI:
10.52711/0974-360X.2023.00355 
Address:
Aqsa Fathima1, Shivaprakash Gangachannaiah1*, Ujjal Bose2, Shama Prasada Kabekkodu3, Rituparna Chakraborty4, Praveen Kumar S E5, Padmanabha Udupa6, Rachagolla Sai Prathap Yadav7, Vidya Monappa8
1Department of Pharmacology, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal, Karnataka, India-576104.
2Department of Pharmacology, American University of Antigua College of Medicine, Coolidge, Antigua P.O. Box 1451.
3Department of Cell and Molecular biology, Manipal School of Life Sciences, Manipal, Manipal Academy of Higher Education, Manipal, Karnataka, India – 576104.
4Department of Microbiology, Melaka Manipal Medical College, Manipal, Manipal Academy of Higher Education, Manipal, Karnataka, India – 576104.
5Department of Pharmacology, Manipal-Tata Medical College Jamshedpur, Manipal Academy of Higher Education, Jharkhand, India – 831017.
6Department of Biochemistry, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal,
Published In:
Volume - 16,
Issue - 5,
Year - 2023
ABSTRACT:
Background: Ulcerative colitis (UC) is an inflammatory bowel disorder affecting the colonic mucosa, characterized by intense inflammation and mucosal damage. The present study aimed to evaluate the protective effect of the Trigonellafoenum-graecum L. (TFG) seeds in acetic acid-induced UC in rats. Materials and Methods: Male rats (n=30) were distributed into 5 groups as normal control, UC, standard, and two test groups. Colitis was induced by acetic acid in all the groups except the normal control group. Normal control and UC group received distilled water, the standard group was administered sulfasalazine at 100mg/kg body weight (bw), and test groups, TFG-I, and TFG-II received TFG seed extract at 500 and 1000 mg/kg bw, respectively. The duration of treatment was 7 days, and colitis was induced on day 8. Animals were sacrificed on day 9 and colonic tissue was dissected and collected for biochemical, molecular, and histological analysis. Results: The disease activity index score in standard, TFG-I, and TFG-II (3.33±0.21, 2.66±0.21, and 3.50±0.22) was significantly lesser (P<0.05) than scores in the UC group (4±0.01). The macroscopic score indicating the intensity of mucosal inflammation was significantly decreased (P= 0.01) to4.0±0.25, 3.16±0.30, and 3.83±0.40 in standard, TFG-I, and TFG-II groups, respectively compared to the UC group (4.66±0.21). Similarly, there was a significant reduction (P=0.05) in histological scores of the standard, TFG-I, TFG-II (3.5±0.34, 1.25±0.34, 3.25±0.34) groups compared to the UC group (4.75±0.34). Biochemical assessment in the standard and test groups showed significant increase (P<0.05) in total protein, reduced glutathione (GSH), catalase (CAT), superoxide dismutase (SOD) levels whereas significant reduction (P<0.01) in malondialdehyde (MDA) levels compared to UC group. The pro-inflammatory cytokine TNF-a levels were significantly decreased (P<0.01) in standard, TFG-I, and TFG-II (-1.75±0.007, -0.27±0.17 and -0.51±0.002) when compared to the UC group (0.20±0.02). Conclusion: The study demonstrates the ability of TFG seeds in reducing the inflammatory and oxidative stress induced mucosal damage in acetic acid-induced UC in rats.
Cite this article:
Aqsa Fathima, Shivaprakash Gangachannaiah, Ujjal Bose, Shama Prasada Kabekkodu, Rituparna Chakraborty, Praveen Kumar S E, Padmanabha Udupa, Rachagolla Sai Prathap Yadav, Vidya Monappa. Effect of aqueous extract of Trigonellafoenum-graecum L. seeds on Acetic acid- induced Ulcerative colitis in rats. Research Journal of Pharmacy and Technology 2023; 16(5):2161-8. doi: 10.52711/0974-360X.2023.00355
Cite(Electronic):
Aqsa Fathima, Shivaprakash Gangachannaiah, Ujjal Bose, Shama Prasada Kabekkodu, Rituparna Chakraborty, Praveen Kumar S E, Padmanabha Udupa, Rachagolla Sai Prathap Yadav, Vidya Monappa. Effect of aqueous extract of Trigonellafoenum-graecum L. seeds on Acetic acid- induced Ulcerative colitis in rats. Research Journal of Pharmacy and Technology 2023; 16(5):2161-8. doi: 10.52711/0974-360X.2023.00355 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2023-16-5-14
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