Author(s):
Sandip Sen, Bairam Ravindar, Sirikonda Jala, Laxmi Dharabonia, Konika Rajeshwari
Email(s):
sandipsen2010@gmail.com
DOI:
10.52711/0974-360X.2023.00368 
Address:
Sandip Sen1*, Bairam Ravindar2, Sirikonda Jala2, Laxmi Dharabonia2, Konika Rajeshwari2
1Department of Pharmaceutical Chemistry, School of Pharmacy,
Guru Nanak Institutions Technical Campus, Ibrahimpatnam, Hyderabad – 501506.
2Department of Pharmaceutical Chemistry, Srikrupa Institute of Pharmaceutical Sciences,
Velikatta, Kondapakka, Siddipet – 502277.
*Corresponding Author
Published In:
Volume - 16,
Issue - 5,
Year - 2023
ABSTRACT:
The present work describes the development of a simple, precise and accurate derivative spectroscopic and RP-HPLC method for the estimation of levofloxacin hemihydrate in the tablet dosage form. Levofloxacin hemihydrate showed absorption maxima at 287.36, 287.2 and 296.2nm in zero, first and second-order spectroscopic methods. The RP-HPLC method was optimized by using Orthophosphoric acid: acetonitrile: methanol (50: 20: 30) as solvent at 287nm. The methods showed linearity in the concentration range 1-5 and 2-10 µg/ ml and the correlation coefficient was 0.999 respectively. The method has been validated according to the ICH guidelines. The percentage relative standard deviation was found to be less than 2% in respect of both methods.
Cite this article:
Sandip Sen, Bairam Ravindar, Sirikonda Jala, Laxmi Dharabonia, Konika Rajeshwari. Derivative Spectroscopic Method and RP-HPLC Method Development and Validation of Levofloxacin hemihydrate. Research Journal of Pharmacy and Technology 2023; 16(5):2239-4. doi: 10.52711/0974-360X.2023.00368
Cite(Electronic):
Sandip Sen, Bairam Ravindar, Sirikonda Jala, Laxmi Dharabonia, Konika Rajeshwari. Derivative Spectroscopic Method and RP-HPLC Method Development and Validation of Levofloxacin hemihydrate. Research Journal of Pharmacy and Technology 2023; 16(5):2239-4. doi: 10.52711/0974-360X.2023.00368 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2023-16-5-27
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