Author(s): Saloni Dalwadi, Vaishali Thakkar, Purvi Shah, Devang Tandel, Kalpana Patel, Hardik Rana

Email(s): dalwadisaloni@gmail.com

DOI: 10.52711/0974-360X.2023.00668   

Address: Saloni Dalwadi1*, Vaishali Thakkar1, Purvi Shah2, Devang Tandel3, Kalpana Patel3, Hardik Rana1
1Department of Pharmaceutics, Anand Pharmacy College, Gujarat Technological University, Anand, 388001, Gujarat, India.
2Dean, Doctoral Study and Research, Parul University, Baroda, 391760, Gujarat, India.
3Department of Quality Assurance, Anand Pharmacy College, Gujarat Technological University, Anand, 388001, Gujarat, India.
*Corresponding Author

Published In:   Volume - 16,      Issue - 9,     Year - 2023


ABSTRACT:
A simple, specific, and quantitative high-performance thin-layer chromatography method has been developed for the simultaneous determination of Glimepiride and 3-acetyl-11-keto-beta-boswellic acidin the formulation. Chromatographic development was performed using n-hexane: Ethyl acetate: Toluene: Glacial acetic acid (4:2:4:0.2, % v/v/v/v) as optimized mobile phase on TLC aluminum plate precoated withsilica gel 60 F254 and further densitometric measurement at 242nm.The drugs were satisfactorily resolved with Rfvalues of 0.38±0.02, 0.65±0.02, for Glimepiride and 3-acetyl-11-keto-beta-boswellic acid, respectively. The linear ranges were found to be 100-600 and 4000-24000ng/band for Glimepiride and 3-acetyl-11-keto-beta-boswellic acid. The accuracy and precision measured were less than 2% relative standard deviation for both drugs. The sensitivity of the method in terms of limit of detection and limit of quantitation were found to be 11.22 and 34ng/band for Glimepiride and 439.92 and 1333.102ng/band for 3-acetyl-11-keto-beta-boswellic acid respectively. 24-1 fractional factorial design was applied for robustness study to evaluate the small difference in independent variables. Among four factors, the significant factor found was the volume of n-hexane that resulted in a change in the Rf of drugs. The proposed method was found to be accurate, precise, reproducible, robust, and specific.


Cite this article:
Saloni Dalwadi, Vaishali Thakkar, Purvi Shah, Devang Tandel, Kalpana Patel, Hardik Rana. High Performance Thin Layer Chromatographic Method for The Simultaneous Quantification of Glimepiride and 3-Acetyl-11-Keto-Beta-Boswellic acid along with Robustness study using Fractional Factorial Design. Research Journal of Pharmacy and Technology 2023; 16(9):4077-4. doi: 10.52711/0974-360X.2023.00668

Cite(Electronic):
Saloni Dalwadi, Vaishali Thakkar, Purvi Shah, Devang Tandel, Kalpana Patel, Hardik Rana. High Performance Thin Layer Chromatographic Method for The Simultaneous Quantification of Glimepiride and 3-Acetyl-11-Keto-Beta-Boswellic acid along with Robustness study using Fractional Factorial Design. Research Journal of Pharmacy and Technology 2023; 16(9):4077-4. doi: 10.52711/0974-360X.2023.00668   Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2023-16-9-12


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