Author(s): Florentina Evelyn Purnomo, Sri Agus Sudjarwo, Suryo Kuncorojakti, Heni Puspitasari, Rofiqul A’la, Andi Yasmin Wijaya, Helen Susilowati, Diyantoro, Nusdianto Triakoso, Boedi Setiawan, Fedik Abdul Rantam

Email(s): fedik-a-r@fkh.unair.ac.id

DOI: 10.52711/0974-360X.2023.00688   

Address: Florentina Evelyn Purnomo1, Sri Agus Sudjarwo2, Suryo Kuncorojakti3,4, Heni Puspitasari4, Rofiqul A’la4, Andi Yasmin Wijaya4, Helen Susilowati4, Diyantoro4,5, Nusdianto Triakoso6, Boedi Setiawan7, Fedik Abdul Rantam4,8*
1Master’s Student, Faculty of Veterinary Medicine, Airlangga University, Surabaya, East Java, 60115, Indonesia.
2Pharmacology Laboratory, Division of Basic Veterinary Science, Faculty of Veterinary Medicine, Airlangga University, Surabaya, East Java, 60115, Indonesia.
3Histology Laboratory, Division of Veterinary Anatomy, Faculty of Veterinary Medicine, Airlangga University, Surabaya, East Java, 60115, Indonesia.
4Research Center for Vaccine Technology and Development, Institute of Tropical Disease, Airlangga University, Surabaya, East Java, 60115, Indonesia.
5Department of Health Science, Faculty of Vocational Studies, Airlangga University, Surabaya, East Java, 60115, Indonesia.
6Internal Medicine Department, Airlangga University Animal Hospital, Faculty of

Published In:   Volume - 16,      Issue - 9,     Year - 2023


ABSTRACT:
Context: Vaccination as prevention to hold back the spread of COVID-19 is important since it is the most concerning health issue in the last decade. Inactivated vaccine platforms considered safer, especially for elderly and comorbid patients. Comorbidities especially Tuberculosis and Hepatitis B, has a major impact to COVID-19 infections and vaccination. To evaluate infections or vaccine response IFN- ? and CD4+ are important. IFN-? has a role in antiviral innate response, including initiation of other cytokines, increasing MHC expression, increasing presentation of macrophage, and increasing presentation of antigen to T cell Naïve. CD4+ is associated with humoral immune response. Cynomolgus Monkey or known as Macaca fascicularis. Specifying to comorbid patients, adult Macaca fascicularis that are detected to have hepatitis B and Tuberculosis (TBC) by PCR were treated as a comorbid group. Adult Macaca fascicularis that used in this research have range from 6 to 9 years old. This study was analysed with SPSS 26 general linear model repeated measures analysis with p<0.05 Objectives: To evaluate IFN- ? and CD4+ response of vaccination Result: The result of this study showed significant (p < 0.05) increase in IFN-? and CD4+ evaluation in both comorbid and adult groups. The elevating concentration and percentage could be the sign of induced humoral and adaptive immune system in the body. Conclusion: SARS-CoV-2 inactivated vaccine candidate that used in this study can increase the number of IFN-? concentration as well as percentage of CD4+ in adult and comorbid groups of Cynomolgus Macaques.


Cite this article:
Florentina Evelyn Purnomo, Sri Agus Sudjarwo, Suryo Kuncorojakti, Heni Puspitasari, Rofiqul A’la, Andi Yasmin Wijaya, Helen Susilowati, Diyantoro, Nusdianto Triakoso, Boedi Setiawan, Fedik Abdul Rantam. Analysis of IFN-γ and CD4+ responses in comorbid and adult immunized cynomolgus monkey with inactivated SARS-CoV-2 vaccine candidate. Research Journal of Pharmacy and Technology 2023; 16(9):4206-2. doi: 10.52711/0974-360X.2023.00688

Cite(Electronic):
Florentina Evelyn Purnomo, Sri Agus Sudjarwo, Suryo Kuncorojakti, Heni Puspitasari, Rofiqul A’la, Andi Yasmin Wijaya, Helen Susilowati, Diyantoro, Nusdianto Triakoso, Boedi Setiawan, Fedik Abdul Rantam. Analysis of IFN-γ and CD4+ responses in comorbid and adult immunized cynomolgus monkey with inactivated SARS-CoV-2 vaccine candidate. Research Journal of Pharmacy and Technology 2023; 16(9):4206-2. doi: 10.52711/0974-360X.2023.00688   Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2023-16-9-32


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