Author(s): Rahadian Zainul, Viol Dhea Kharisma, Santika Lusia Utami, Nelson Chandra, Arif Nur Muhammad Ansori, Arli Aditya Parikesit, Vikash Jakhmola, Daimon Syukri, Edi Syafri, Asri Peni Wulandari, Oski Illiandri, Khoirun Nisyak, Bahrun, Asmi Citra Malina A. R. Tasakka

Email(s): rahadianzmsiphd@fmipa.unp.ac.id

DOI: 10.52711/0974-360X.2024.00150   

Address: Rahadian Zainul1,2*, Viol Dhea Kharisma3, Santika Lusia Utami4, Nelson Chandra5, Arif Nur Muhammad Ansori6,7, Arli Aditya Parikesit5, Vikash Jakhmola7, Daimon Syukri8, Edi Syafri9, Asri Peni Wulandari10,11, Oski Illiandri12, Khoirun Nisyak13, Bahrun14, Asmi Citra Malina A. R. Tasakka15
1Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang, Padang, Indonesia.
2Center for Advanced Material Processing, Artificial Intelligence, and Biophysic Informatics(CAMP-BIOTICS), Universitas Negeri Padang, Padang, Indonesia.
3Computational Virology Research Unit, Division of Molecular Biology and Genetics, Generasi Biologi Indonesia Foundation, Gresik, Indonesia.
4Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, Indonesia.
5Bioinformatics Department, Indonesia International Institute of Life Sciences (i3L), Jakarta Timur, Indonesia.
6Postgraduate School, Universitas Airlangga, Surabaya, Indonesia.
7Uttaranchal Institute of Pharmaceutical

Published In:   Volume - 17,      Issue - 3,     Year - 2024


ABSTRACT:
Nowadays, dengue virus (DENV) is still become a global problem, even though the virus infection issues have reached half of the population in some countries each year. DENV belongs to the enveloped virus with positive-sense single-stranded RNA (+ssRNA) genus Flavivirus and belongs to the Flaviviridae family. DENV has structural proteins which consist of the envelope protein (E), capsid (C), and membrane (M). There are four serotypes of this virus which are DENV-1, 2, 3, and 4. These four serotypes are transmitted to humans through Aedes sp. The development of this vaccine is still in progress and the challenge of this DENV vaccine candidate design is to overcome the heterotypic infection and the expansion of coverage protection to all virus serotypes. This research uses design simulation for vaccine candidates using B cell epitope in all DENV’s serotypes envelope to trigger the antibody formation through bioinformatics method that consists of protein modeling, immunogenicity, toxicity, and immune stimulation. DENV envelope protein was predicted to have polytope that can be recognized by B cells and act as an antigen, have low similarity with the composing sequence of cell surface receptors on the body, and non-toxic, and then can trigger the population increase of B cell and IgM antibody production with high avidity to neutralize four of the DENV serotypes. We recommend the B cell polytype which consists of A, C, E, and G peptides be examined by the wet-lab approach.


Cite this article:
Rahadian Zainul, Viol Dhea Kharisma, Santika Lusia Utami, Nelson Chandra, Arif Nur Muhammad Ansori, Arli Aditya Parikesit, Vikash Jakhmola, Daimon Syukri, Edi Syafri, Asri Peni Wulandari, Oski Illiandri, Khoirun Nisyak, Bahrun, Asmi Citra Malina A. R. Tasakka. A Viroinformatics Study: B-Cell Polytope Mapping of Envelope Protein to Develop Vaccine Candidate against Four DENV Serotype. Research Journal of Pharmacy and Technology. 2024; 17(3):973-8. doi: 10.52711/0974-360X.2024.00150

Cite(Electronic):
Rahadian Zainul, Viol Dhea Kharisma, Santika Lusia Utami, Nelson Chandra, Arif Nur Muhammad Ansori, Arli Aditya Parikesit, Vikash Jakhmola, Daimon Syukri, Edi Syafri, Asri Peni Wulandari, Oski Illiandri, Khoirun Nisyak, Bahrun, Asmi Citra Malina A. R. Tasakka. A Viroinformatics Study: B-Cell Polytope Mapping of Envelope Protein to Develop Vaccine Candidate against Four DENV Serotype. Research Journal of Pharmacy and Technology. 2024; 17(3):973-8. doi: 10.52711/0974-360X.2024.00150   Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2024-17-3-1


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