Author(s): Ganesh Choudhari, Vishnu Choudhari, Chandrakant More, Laxman Khatal, Vaishnavi Nathe


DOI: 10.52711/0974-360X.2024.00193   

Address: Ganesh Choudhari*1,2, Vishnu Choudhari1,2, Chandrakant More3, Laxman Khatal2, Vaishnavi Nathe1,2
1School of Pharmacy, Dr. Vishwanath Karad MIT World Peace University, MIT Campus, Kothrud, Pune - 411038, Maharashtra, India.
2School of Health Sciences and Technology,
Dr. Vishwanath Karad MIT World Peace University, MIT Campus, Kothrud, Pune - 411038, Maharashtra, India.
3Shri Swami Samarth Ayurvedic Pharmaceuticals, Trimbakeshwar, Dist. Nashik - 422212, Maharashtra, India.
4Shivaji University, Vidyanagar, Kolhapur - 416 004, Maharashtra, India.
*Corresponding Author

Published In:   Volume - 17,      Issue - 3,     Year - 2024

The antidiabetic and antihyperlipidemic herbal preparations were developed in liquid and oral tablet dosage form as Saptarangi Plus Kadha (SK) and Saptarangi Tablet (ST). Streptozotocin (STZ) (45mg/kg) was injected by single intraperitoneal (i.p.) injection and diabetes was induced in Wistar rats of group II to group VII. Metformin (100mg/kg, p.o.) was co-administered orally to group IV to VII with SK and ST as a reference standard drug. There is a need of bio-analytical study and standardization of herbal preparation which is required to get approval of the regulatory agencies for the commercialization. The estimation of metformin was carried in the presence of other herbal phytoconstituents by LCMS/MS. The samples of all the groups were centrifuged and vortexed. The samples were prepared in ACN: 0.1% formic acid (70:30) and LCMS method was developed and validated. The metformin was estimated in presence of active phytoconstituents of SK and ST. SK and ST contains Salacinol and folia salaciosides E1 which was detected by LCMS at m/z 333.42 and m/z 504.63 respectively. There has been an honest effort to create and validate LCMS method for the detection of metformin in presence of phytoconstituents of SK and ST in serum.

Cite this article:
Ganesh Choudhari, Vishnu Choudhari, Chandrakant More, Laxman Khatal, Vaishnavi Nathe. Bio-analytical Estimation of Metformin Co-administered with Antidiabetic and Antihyperlipidemic Herbal Preparations by LCMS. Research Journal of Pharmacy and Technology. 2024; 17(3):1240-5. doi: 10.52711/0974-360X.2024.00193

Ganesh Choudhari, Vishnu Choudhari, Chandrakant More, Laxman Khatal, Vaishnavi Nathe. Bio-analytical Estimation of Metformin Co-administered with Antidiabetic and Antihyperlipidemic Herbal Preparations by LCMS. Research Journal of Pharmacy and Technology. 2024; 17(3):1240-5. doi: 10.52711/0974-360X.2024.00193   Available on:

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