Author(s): Candra Irawan, Foliatini, Cysilia K. Hindarto, Riri Enriyani, Maman Sukiman, Bening Prastiwi


DOI: 10.52711/0974-360X.2024.00154   

Address: Candra Irawan1, Foliatini1*, Cysilia K. Hindarto2, Riri Enriyani2, Maman Sukiman3, Bening Prastiwi1
1Food Nanotechnology Study Program, Polytechnic of AKA Bogor.
2Chemical Analysis Study Program, Polytechnic of AKA Bogor.
3Industrial Waste Treatment, Polytechnic of AKA Bogor.
*Corresponding Author

Published In:   Volume - 17,      Issue - 3,     Year - 2024

Phytochemicals from natural product such as part of plants have been widely used as a potential source of herbal medication due to their bioactivity. Ketepeng cina (Senna alata L) is a small shrub that lives in tropical and subtropical regions and commonly used as medicinal plant to cure various diseases from infectious microbes. The current research was aimed to evaluate the extraction effectivity of Senna alata L leaves and the influence of the parameter to the total phenolic content, antioxidative, antimicrobial and antigout activity. Extraction of Senna alata L leaves was conducted by several methods, i.e maceration, ultrasonication and hot water extraction. The study revealed that each method give different yield of extraction and the most effective among those method is maceration, which produce highest yields of extracts (19.4%). Ultrasonic-assisted extraction (UAE) method also resulted in high yield but lower than that of maceration. The maceration method increases the yield by about 170% compared to hot water method. The extracts obtained from maceration method contain highest content of total phenolic (278.39mg GAE/g dry mass), thus demonstrate highest antioxidant activity (IC50 25.68mg/L), but lowest anti uric activity (reducing uric acid level by 85.25%). Maceration method was able to increase total phenolic content by about 128% and improve antioxidant activity by about 41%, compared to hot water method. However, UAE was excellent in producing phytochemicals which were responsible in anti uric activity, thus have the highest percentage in reducing UA level (90.72%), or increases by about 6.4%. compared to hot water method.

Cite this article:
Candra Irawan, Foliatini, Cysilia K. Hindarto, Riri Enriyani, Maman Sukiman, Bening Prastiwi. Effect of Extraction effectivity of Senna alata L. Leaves on the Total Phenolic, Antioxidative, Antimicrobial and Antigoat Activity. Research Journal of Pharmacy and Technology. 2024; 17(3):997-2 doi: 10.52711/0974-360X.2024.00154

Candra Irawan, Foliatini, Cysilia K. Hindarto, Riri Enriyani, Maman Sukiman, Bening Prastiwi. Effect of Extraction effectivity of Senna alata L. Leaves on the Total Phenolic, Antioxidative, Antimicrobial and Antigoat Activity. Research Journal of Pharmacy and Technology. 2024; 17(3):997-2 doi: 10.52711/0974-360X.2024.00154   Available on:

1.    Gritsanapan W. Mangmeesri P. Standardized Senna Alata Leaf Extract. J. Health Res. 2009; 23 (2):59
2.    Yacob T. Endriani R. Daya Antibakteri Ekstrak Etanol Ketepeng Cina (Senna alata) Terhadap Staphylococcus aureus dan Escherichia coli Secara in Vitro (Antibacterial Power of Ethanol Extract of Chinese Ketepeng (Senna alata) Against Staphylococcus aureus and Escherichia coli in Vitro). Natural Indonesia. 2010; 13 (1):63.
3.    Grosvenor PW. Gothard PK. Mc.William NC. Supriona A. Gray DO. Medicinal plants from Riau province, Sumatra, Indonesia Part I; uses. J Ethnopharmacol. 1995; 45 (2):75.
4.    Sule WF. Okonko IO. Joseph TA. Ojezele M. Nwanze JC. Alli JA. Adewale OG. Ojezele OJ. In Vitro Antifungal Activity of Senna alata Crude Leaf Extract. Biological Sciences. 2010; 5 (3):275
5.    Bahroini FAF. Potensi ekstrak daun ketepeng cina (Cassia alata) sebagai pencegahan stres oksidatif (Potency of ketepeng cina (Cassia alata) leaves extract as oxidative stress prevention). Skripsi (Thesis). 2021; Fakultas Kedokteran, Universitas Muhammadiyah Malang, Indonesia
6.    Ehiowemwenguan G. Inetianbor JE. Yakubu JM. Antimicrobial Qualities of Senna Alata. IOSR Journal of Pharmacy and Biological Sciences. 2014; 9 (2) 47
7.    Fatmawati S. Yuliana. Purnomo AS. Abu Bakar MF. Chemical constituents, usage and pharmacological activity of Cassia alata, Heliyon. 2020; 6 (7): e04396. doi: 10.1016/j.heliyon.2020.e04396
8.    Lim T.K. “Senna alata” in book: edible medicinal and non-medicinal plants. 2014; 7:841. doi:10.1007/978-94-007-7395-0_68
9.    Zhang QW. Lin GL. Ye WC. Techniques for extraction and isolation of natural products: a comprehensive review. Chin Med. 2018; 13:20.
10.    Yeo YL. Chia YY. Lee CH. Sow HS. Yap WS. Effectiveness of Maceration Periods with Different Extraction Solvents on in-vitro Antimicrobial Activity from Fruit of Momordica charantia L. Journal of Applied Pharmaceutical Science. 2014; 4 (10): 016.doi: 10.7324/JAPS.2014.401004
11.    Lee JW. Park HY. Park J. Enhanced Extraction Efficiency of Flavonoids from Pyrus ussuriensis Leaves with Deep Eutectic Solvents. Molecules. 2022; 27 (9):2798.doi: 10.3390/molecules27092798
12.    Campos L. Seixas L. Dias S. Peres AM. Veloso ACA. Henriques M. Effect of Extraction Method on the Bioactive Composition, Antimicrobial Activity and Phytotoxicity of Pomegranate By-Products. Foods. 2022; 11 (7): 992. 11070992
13.    Giovagnoli-Vicuña C. Briones-Labarca V. Romero MS. Giordano A. Pizarro S. Effect of Extraction Methods and In Vitro Bio-Accessibility of Microencapsulated Lemon Extract. Molecules. 2022; 27 (13): 4166.
14.    Glevitzky I. Dumitrel GA. Glevitzky M. Pasca B. Otrisal P. Bungau S. Cioca G. Pantis C. Popa M. Statistical Analysis of the Relationship Between Antioxidant Activity and the Structure of Flavonoid Compounds. Rev.Chim.(Bucharest). 2019; 70 (9)
15.    Irawan C. Sukiman M. Ismail. Putri ID. Utami A. Dewanta A. Optimization of the Ultrasound Assisted Extraction of Phaleria macrocarpa (Scheff.) Boerl. Fruit Peel and its Antioxidant and Anti-Gout Potential. Pharmacogn J. 2022; 14 (2):397
16.    Abubakar AR. Haque M. Preparation of Medicinal Plants: Basic Extraction and Fractionation Procedures for Experimental Purposes. J Pharm Bioallied Sci. 2020; 12 (1):1
17.    Carreira-Casais A. Otero P. Garcia-Perez P. Garcia-Oliveira P. Pereira AG. Carpena M. Soria-Lopez A. Simal-Gandara J. Prieto MA. Benefits and Drawbacks of Ultrasound-Assisted Extraction for the Recovery of Bioactive Compounds from Marine Algae, Int J Environ Res Public Health. 2021; 18 (17): 9153. doi: 10.3390/ijerph18179153.
18.    Patil SP. Laddha KS. Extraction Efficiency of Agnuside from Vitex Negundo Leaves Using Different Techniques and its Quantitative Determination by HPLC, International Journal of Health Sciences & Research. 2018; 8 (8)
19.    Okitsu K. Cavalieri F. Sonochemical Production of Nanomaterials in Springer Briefs In Molecular Science: Ultrasound And Sonochemistry, Springer Nature Switzerland. 2018
20.    Kumar K. Srivastava S. Sharanagat VS. Ultrasound assisted extraction (UAE) of bioactive compounds from fruit and vegetable processing by-products: A review, Ultrason Sonochem. 2021; 70 :105325. doi: 10.1016/j.ultsonch.2020.105325
21.    Wang L. Weller CL. Recent Advances in Extraction of Nutraceuticals from Plants. Trends in Food Science & Technology. 2006; 17:300.
22.    Vinatoru M. Mason TJ. Calinescu I. Ultrasonically assisted extraction (UAE) and microwave assisted extraction (MAE) of functional compounds from plant materials. TrAC - Trends Anal Chem. 2017; 97:159
23.    Nurhadi B. Saputra RA. Setiawati TA. Husein SN. Faressi FR. Utari CD. Sukri N. Kayaputri IL. Setiasih IS. Comparison of Curcuma domestica and Curcuma xanthorrhiza oleoresins extracted using maceration, Soxhlet, and ultrasound-assisted extraction (UAE), IOP Conf. Ser.: Earth Environ. Sci. 2020; 443:012074
24.    Grdiša M. Varga F. Ninčević T. Ptiček B. Dabić D. Biošić M. The extraction efficiency of maceration, UAE and MSPD in the extraction of pyrethrins from Dalmatian pyrethrum. Agric. Conspec. Sci. 2020; 85 (3):257
25.    Lamuela-Raventós RM. Folin–Ciocalteu method for the measurement of total phenolic content and antioxidant capacity, in Measurement of Antioxidant Activity & Capacity: Recent Trends and Applications Editor(s):Resat Apak, Esra Capanoglu, Fereidoon Shahidi. John Wiley & Sons Ltd. 2018; doi:10.1002/9781119135388
26.    Nofita SD. Ngibad K. Rodli AF. Determination Of Percentage Yield And Total Phenolic Content Of Ethanol Extract From Purple Passion (Passiflora edulis f. edulis Sims) FRUIT PEEL. J. Pijar MIPA. 2022; 17 (3):309.doi: 10.29303/jpm.v17i3.3461
27.    Mutha RE. Tatiya AU. Surana SJ. Flavonoids as natural phenolic compounds and their role in therapeutics: an overview, Futur J Pharm Sci. 2021; 7 (1): 25.doi: 10.1186/s43094-020-00161-8
28.    Šamec D. Karalija E. Šola I. Bok VV. Salopek-Sondi B. The role of polyphenols in abiotic stress response: The influence of molecular structure. Plants. 2021; 10 :118.doi: 10.3390/plants10010118.
29.    Dias MC. Pinto DCGA. Silva AMS. Plant Flavonoids: Chemical Characteristics and Biological Activity. Molecules. 2021; 26 (17): 5377.doi: 10.3390/molecules26175377
30.    Styani E. Hanafi. Sulistiawaty L. Irawan C. Putri ID. Liquid Chromatograph-Mass Spectrophotometer and Anti Uric Acid Potential Studies of Ethyl Acetate Extract of Archidendron bubalinum (Jack) I.C. Nielsen Fruit Seed Shell. International Conference on Science and Technology. 2018;293.
31.    Liu L. Zhang L. Ren L. Xie Y. Advances in structures required of polyphenols for xanthine oxidase inhibition. First published: 2020; doi:10.1002/fft2.27
32.    Sharma L. Ashraf Z. The role of xanthine oxidase inhibiting foods in therapeutic management of hyperuricemia and gout. International Journal of Research in Social Sciences. 2018; 8(11), 253.  

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