Author(s): Komal Somkuwar, Prafulla Sabale, Vaibhav Sawale, Priya Rahangdale

Email(s): sweetsomkuwar@gmail.com

DOI: 10.52711/0974-360X.2024.00205   

Address: Komal Somkuwar*, Prafulla Sabale, Vaibhav Sawale, Priya Rahangdale
Department of Pharmaceutical Sciences, R. T. M. Nagpur University, Mahatma Jyotiba Fuley Educational Campus, Amravati Road, Nagpur - 440033, Maharashtra, India.
*Corresponding Author

Published In:   Volume - 17,      Issue - 3,     Year - 2024


ABSTRACT:
Umifenovir, an antiviral drug that is used to treat influenza, has recently been used in COVID-19 infection. According to a literature survey, no UV technique for the estimation of umifenovir has yet been established; hence, there is an imperative need for a simple analytical method. Additionally, we developed an alternative, RP-HPLC method for the estimation of umifenovir. UV spectrophotometry was carried out at 223nm absorption maxima using the solvent methanol. A concentration range of 2-12µg/ml was found to obey Beer's Law, with a correlation coefficient (r2) of 0.9995. A C-18 column (250mm 4.6µm, 5µm) was used for chromatographic separation using isocratic mode. The mixture consisted of acetonitrile: 0.1% trimethylamine (pH adjusted to 2.7 by the addition of orthophosphoric acid) 60:40 as the mobile phase with a flow rate of 1.0 ml/min. The temperature was kept at 25ºC, and detection at 223nm was performed using a PDA detector. The estimated percentage of the drug was close to 100%, corresponding to the label claim of the tablet made in the laboratory. The results and statistical study demonstrated the utility of the current methods in the routine evaluation of umifenovir bulk and formulation.


Cite this article:
Komal Somkuwar, Prafulla Sabale, Vaibhav Sawale, Priya Rahangdale. Method Development and Validation for the Estimation of Umifenovir in Bulk and Tablet Formulation by UV-Spectrophotometric and RP-HPLC. Research Journal of Pharmacy and Technology. 2024; 17(3):1308-3. doi: 10.52711/0974-360X.2024.00205

Cite(Electronic):
Komal Somkuwar, Prafulla Sabale, Vaibhav Sawale, Priya Rahangdale. Method Development and Validation for the Estimation of Umifenovir in Bulk and Tablet Formulation by UV-Spectrophotometric and RP-HPLC. Research Journal of Pharmacy and Technology. 2024; 17(3):1308-3. doi: 10.52711/0974-360X.2024.00205   Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2024-17-3-56


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