Author(s): Ravi Kumar Annepu, Subhashini Devi Prattipati

Email(s): E-mail:

DOI: 10.52711/0974-360X.2024.00319   

Address: Ravi Kumar Annepu, Subhashini Devi Prattipati* Department of Biochemistry, Andhra University, Visakhapatnam - 530 003.
*Corresponding Author

Published In:   Volume - 17,      Issue - 5,     Year - 2024

In the present study the antioxidant potential of three seaweeds Gracileria corticata (red algae), Ulva lactuca (green algae) and Sargassum ilicifolum (brown algae) were reported. SDS-PAGE analysis reveals that total of 23 different protein bands were observed of this 16, 14 and 17 bands in U. lactuca, S. ilicifolium and G. Corticata respectively. The chloroform, acetone and methanolic extracts of three algae showed the concentration dependent DPPH, FRAP, reducing power, H2O2 radical scavenging and metal chelating activities. The methanolic extracts of G. corticata have high levels of antioxidants with high DPPH, FRAP, reducing power activities and S. ilicifolum have high H2O2 radical scavenging, and metal chelating activities.G. corticata showed IC50 value for 902.09µg/ml for DPPH activity, 2274µg/ml for FRAP activity, 1082.11µg/ml for H2O2 radical scavenging. Among the three solvents tested, Methanol was found to be the most suitable solvent for the extraction of compounds.

Cite this article:
Ravi Kumar Annepu, Subhashini Devi Prattipati. Antioxidant potential of three different Sea weeds from Visakhapatnam Coast, India. Research Journal of Pharmacy and Technology. 2024; 17(5):2018-4. doi: 10.52711/0974-360X.2024.00319

Ravi Kumar Annepu, Subhashini Devi Prattipati. Antioxidant potential of three different Sea weeds from Visakhapatnam Coast, India. Research Journal of Pharmacy and Technology. 2024; 17(5):2018-4. doi: 10.52711/0974-360X.2024.00319   Available on:

1.    Vishnupriya Sowjanya I. Raja Sekhar PS. A comparative study of seaweed flora over a period of time with reference to climate change in Visakhapatnam Coast, Andhra Pradesh, India. Advances in Applied Science Research. 2015; 6(6):187-193.
2.    Chopin T. Tacon AG. Importance of Seaweeds and Extractive Species in Global Aquaculture Production. Reviews in Fisheries Science and Aquaculture. 2021; 29(2): 139-148.
3.    Collins KG. Fitzgerald GF. Stanton C. Ross RP. Looking beyond the terrestrial: The potential of seaweed derived bio-actives to treat non-communicable diseases. Marine Drugs. 2016; 14(3): 60. doi: 10.3390/md14030060.
4.    Michalak I. Chojnacka K. Algae as production systems of bioactive compounds. Engineering in Life Sciences. 2015; 15: 160-176.
5.    Varshini R. Suganyadevi P. Hariprasath L. Antioxidant potential of three selected seaweeds Sargassum wightii, Turbinaria conodies, Padina tetrostromata from Indian coastal area. Research Journal of Biotechnology. 2023; 18(2): 22-35.
6.    Pereira L. Seaweeds as source of bioactive substances and skin care therapy-cosmeceuticals, algotheraphy and thalassotherapy. Cosmetics. 2018a; 5(4): 68.
7.    Sobuj MKA. Islam M.A. Islam MS. Islam MM, Mahmud Y. Rafiquzzaman SM. Effect of solvents on bioactive compounds and antioxidant activity of Padina tetrastromatica and Gracilaria tenuistipitata seaweeds collected from Bangladesh. Scientific Reports. 2021; 11, 19082.
8.    Liu R. Mabury SA. Synthetic phenolic antioxidants: A review of environmental occurrence, fate, human exposure, and toxicity. Environmental Science and Technology. 2020; 54: 11706–11719.
9.    Rafiquzzaman SM. Kim EY. Kim YR. Nam TJ. Kong IS. Antioxidant activity of glycoprotein purified from Undaria pinnatifida measured by an in vitro digestion model. International Journal of Biological Macromolecules. 2013; 62: 265–272. doi: 10.1016/j.ijbiomac.2013.09.009
10.    Jenifer P. Balakrishnan CP. Chidambaram Pillai S. In-vitroAntioxidant activity of Marine Red Algae Gracilaria foliifera. Asian Journal of Pharmacy and Technology. 2017; 7( 2): 105-108.
11.    Roy Amit. Bhoumik Dayananda. Sahu Ram Kumar. Dwivedi Jaya. Phytochemical Screening and Antioxidant Activity of Sesbania grandiflora leaves extracts. Asian Journal of Research in Pharmaceutical Science. 2014; 4(1): 16-21.
12.    Souad Belakehal. Brahim Labed. Louiza Zenkhri. Khedidja Benzahi. Ahmed Tabchouche. In vitro comparative study on the antibacterial and the antioxidant activity of Pergularia tomentosa L. Asian Journal of Research in Chemistry. 2021; 14(4): 285-291. doi: 10.52711/0974-4150.2021.00049.
13.    Chouikh A. Houba Z. Himeur H. Alia F. Adjal E. Phytochemical Study, HPLC Chromatographic Analysis and Antioxidant Activity of Ephedra alataDC. Female Cones Extracts. Asian Journal of Research in Chemistry. 2021; 14(4): 259-264. DOI: 10.52711/0974-4150.2021.00044
14.    Habchi A. Dekmouche M. Hamia C.  Saidi M. Yousfi M. Bouguerra A. Extraction of phenolic compounds of six Algerian date (Phoenix dactylifera L.) cultivars from Ain-Saleh region, using reflux method and screening of antioxidant activity in vitro. Asian Journal of Research in Chemistry. 2021; 14(3): 161-167. DOI: 10.52711/0974-4150.2021.00030.
15.    Akshay R Yadav. Shrinivas K Mohite. Manisha D Rajput. Vaibhav S Suryawanshi. Rushikesh M Birajdar. Mayuri V Patil. Antioxidant Activity of Psidiumguajava Leaf Extracts. Research Journal of Pharmaceutical Dosage Forms and Technology. 2020; 12(3): 159-161. DOI: 10.5958/0975-4377.2020.00027.0
16.    Preeti Yadav. Abhishek Kumar Jain. Gaurav Kumar. Loganathan Karthik. Kokati Venkata Bhaskara Rao. Phytochemical composition and antioxidant activity of Ficuselastica Roxb. (Moraceae) leaves. Research Journal of Pharmacy and Technology. 2015; 8(3): 259-264. DOI: 10.5958/0974-360X.2015.00043.8.
17.    Avinash Suryawanshi. Pushplata Chougule. Ashish Jain. In-vitro antioxidant activity of hexanolic extract of Trachyspermumammi. Research Journal of Pharmacy and Technology. 2015; 8(10): 1403-1408. doi: 10.5958/0974-360X.2015.00252.8
18.    Sathyapriya Murugan. Aswini Shanmugam. Lakshmi Manoharan. Shoba Sundaramoorthy. Shylaja Gunasekaran. Sathiavelu Arunachalam. Mythili Sathiavelu. Antioxidant activity of aqueous and methanol extract of Barnyard millet. Research Journal of Pharmacy and Technology. 2016; 9(3): 262-266. doi: 10.5958/0974-360X.2016.00048.2
19.    Ramandeep Kaur. Navneet Kumar. Phytochemical Composition and in vitro Antioxidant Activity of Leucasaspera Leaves. Research Journal of Pharmacy and Technology. 2016; 9(12): 2217-2221. doi: 10.5958/0974-360X.2016.00448.0
20.    Gunathilaka TL. Samarakoon K. Ranasinghe P. Peiris DC. Antidiabetic potential of marine brown algae-a mini review. J Diabetis Res. 2020; Apr 25; 2020: 1230218. doi: 10.1155/2020/1230218.
21.    Arnold TM. Targett NM. Quantifying in situ rates of phlorotannin synthesis and polymerization in marine brown algae. Journal of Chemical Ecology. 1998; 24: 577-595.
22.    Prasanna Lakshmi K, Narasimha Rao GM. Some Numerical studies on Marine algae of Visakhapatnam Coast. Journal of Algal Biomass Utilization. 2009; (1): 60-85.
23.    Fatchiyah. Arumingtyas EL. Widyarti S. Rahayu S. Biologimolekuler: prinsipdasaranalisis. Jakarta: Penerbit Erlangga. 2011.
24.    Huang DJ. Ou B. Prior RL. The chemistry behind antioxidant capacity assays. Journal of Agriculture and Food Chemistry. 2005; 53(6): 1841-1856. doi: 10.1021/jf030723c.
25.    Lim SN, Cheung PC, Ooi VE, Ang PO. Evaluation of antioxidative activity of extractsfrom brown seaweed, Sargassum siliquastrum. Journal of Agriculture and Food Chemistry. 2002; 50(13): 3862-3866.
26.    Ruch R. Cheng SJ. Klaunig JE. Prevention of cytotoxicity and inhibition of intracellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogenesis. 1989; 10: 1003-1008. doi: 10.1093/carcin/10.6.1003.
27.    Dinis TC. Maderia VM. Almeida LM. Action of phenolic derivatives (acetaminophen, salicylate, and 5-aminosalicylate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Archives of Biochemistry and Biophysics. 1994; 315(1): 161-169. doi: 10.1006/abbi.1994.1485. PMID: 7979394.
28.    Vijayalakshmi M. Ruckmani K. Ferric reducing anti-oxidant power assay in plant extract. Bangladesh Journal of Pharmacology. 2016; 11: 570-572. doi:
29.    Wong SP. Leong PL. Koh JHW. Antioxidant activities of aqueous extracts of selected plants. Food Chemistry. 2006; 99: 775-783.
30.    Senevirathne M. Kim SH. Siriwardhana N. Ha JH. Lee KW. Jeon YJ. Antioxidant potential of Ecklonia cava on reactive oxygen species scavenging, metal chelating, reducing power and lipid peroxidation inhibition. Food Science and Technology International. 2006; 12(1):27-38. doi:10.1177/1082013206062422
31.    Heo SJ. Cha SH. Lee KW. Jeon YJ. Antioxidant activities of red algae from Jeju Island. Algae. 2006; 21(1): 149-156. doi:
32.    Chakraborty K. Joseph D. Praveen NK. Antioxidant activities and phenolic contents of three red seaweeds (Division: Rhodophyta) harvested from the Gulf of Mannar of Peninsular India. Journal of Food Science and Technology. 2015; 52(4): 1924-1935. doi: 10.1007/s13197-013-1189-2.
33.    Gómez-Guzmán M. Rodríguez-Nogales A. AlgieriF,Gálvez J. Potential role of seaweed polyphenols in cardiovascular-associated disorders. Marine Drugs. 2018; 16(8): 250.doi: 10.3390/md16080250.
34.    Turkmen N. Sari F. Velioglu YS. Effects of extraction solvents on concentration and antioxidant activity of black and black mate tea polyphenols determined by ferrous tartrate and Folin-Ciocalteu methods. Food Chemistry. 2006; 99(4): 835–841.
35.    Kuppusamy P. Yusoff MM. Parine NR. Govindan N. Evaluation of in vitro antioxidant and antibacterial properties of CommelinanudifloraL. extracts prepared by different polar solvents. Saudi Journal of Biological Sciences. 2015; 22(3): 293-301.doi: 10.1016/j.sjbs.2014.09.016.
36.    Gong L. Ramm G. Devenish RJ. Prescott M. HcRed, a genetically encoded fluorescent binary cross-linking agent for cross-linking of mitochondrial ATP synthase in Saccharomyces cerevisiae. PLOS ONE. 2012; 7(4): e35095.
37.    Wang BG. Zhang WW. Duan XJ. Li XM. In vitro antioxidative activities of extract and semi-purified fractions of the marine red alga, Rhodomelaconfervoides (Rhodomelaceae). Food Chemistry. 2009; 113: 1101-1105.
38.    Truong DH. Ta NTA. Pham TV. Huynh TD. Do QTG. Dinh NCG. Dang CD. Nguyen TKC. Bui AV. Effects of solvent-solvent fractionation on the total terpenoid content and in vitro anti-inflammatory activity of Sereveniabuxifolia bark extract. Food Science and Nutrition. 2021; 9: 1720-1735. doi: 10.1002/fsn3.2149.

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