Author(s):
Lilis Sulistiawaty, Maulidiawati Khoirunisa, Imas Solihat, M. Nashih
Email(s):
imaskhairani@gmail.com
DOI:
10.52711/0974-360X.2024.00534 
Address:
Lilis Sulistiawaty1, Maulidiawati Khoirunisa1, Imas Solihat2*, M. Nashih3
1Department of Chemical Analysis, Politeknik AKA Bogor, Jalan Pangeran Sogiri, Tanah Baru, Bogor 16154, Indonesia.
2Department of Food Nanotechnology, Politeknik AKA Bogor, Jalan Pangeran Sogiri, Tanah Baru, Bogor 16154, Indonesia.
3LPPOM, MUI Indonesia, Jl. Pemuda No.5 Tanah Sereal, Sempur Bogor 16161 Indonesia.
*Corresponding Author
Published In:
Volume - 17,
Issue - 7,
Year - 2024
ABSTRACT:
Determination of ethanol content on kombucha tea refers to AOAC 2016.12 using an internal standard method and by gas chromatography and is different from inhouse method. Validation parameter that performed was linearity (R2>0,990), precision (%RSD <2/3CV Horwitz), accuracy (%recovery: 95-105%), instrument detection limit (0,0015%), quantitation detection limit (0,0086%) and method detection limit (0,0328%) and these numbers has met the acceptance requirements so that this method can be used for routine analysis in the laboratory.
Cite this article:
Lilis Sulistiawaty, Maulidiawati Khoirunisa, Imas Solihat, M. Nashih. Determination of Ethanol in Kombucha with Gc-Fid: A Validated Method for Halal Verification. Research Journal of Pharmacy and Technology. 2024; 17(7):3415-0. doi: 10.52711/0974-360X.2024.00534
Cite(Electronic):
Lilis Sulistiawaty, Maulidiawati Khoirunisa, Imas Solihat, M. Nashih. Determination of Ethanol in Kombucha with Gc-Fid: A Validated Method for Halal Verification. Research Journal of Pharmacy and Technology. 2024; 17(7):3415-0. doi: 10.52711/0974-360X.2024.00534 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2024-17-7-67
REFERENCE:
1. Jarrell J. Cal T. Bennett JW. The Kombucha consortia of yeasts and bacteria. Mycologist. 2000; 14(4): 166–170.
2. [BSN] Standar Nasional Indonesia 8965. Tentang pengukuran alkohol dalam minuman, Badan standardisasi nasional. Jakarta. 2021.
3. Jayabalan R. Malbaša R. Lončar ES. Vitas JS. Sathishkumar M. A review on Kombucha tea-microbiology, composition, fermentation, beneficial effects, toxicity, and tea fungus. Comprehensive Reviews in Food Science and Food Safety. 2014; 13(4): 538–550.
4. Greenwalt CJ. Steinkraus KH. Ledford RA. Kombucha, the fermented tea: Microbiology, composition, and claimed health effects. Journal of Food Protection. 2000; 63(7): 976–981.
5. May A. Narayanan S. Alcock J. Varsani A. Maley C. Aktipis A. Kombucha: a novel model system for cooperation and conflict in a complex multi-species microbial ecosystem. PeerJ. 2019; 1–22.
6. Endrawati S. Artanti N. Hanafi M. Antioxidant Activity and Compounds Identification of Sauropus androgynus (L.) Merr. and Moringa oleifera Lam Leaves Combination Fermented with Kombucha Consortium. Research Journal of Pharmacy and Technology. 2022; 15(11): 5184-1.
7. Kapp JM. Sumner W. Kombucha: A systematic review of the empirical evidence of human health benefit. Annals of Epidemiology. 2019; 30: 66–70.
8. Mansur A. Oh J. Lee HS. Oh SY. Determination of ethanol in foods and ebverages by magnetic stirring-assisted aqueous extraction coupled with GC-FID: A validated method for halal verification. Food Chemistry. 2022; 366-130526.
9. LPPOM MUI. Panduan Umum Sistem Jaminan Halal LPPOM-MUI. 2018.
10. Mornar A. Serti´c M. Klari´c DA. Klari´c I. Stipanovi´c K. Nigovi´c B. Evaluation of alcohol content and metal impurities in liquid dietary supplements by sHSS-GC-FID and GFAAS techniques. Food Chemistry. 2016; 211: 285–293.
11. Ebersole B. Liu Y. Schmidt R. Eckert M. Brown PN. Determination of ethanol in kombucha product: single-laboratory validation, first action 2016.12. Journal of AOAC International. 2017; 100(3).
12. Biswas B. Kumar B. Sharma JB. Saini V. Bhatt S. Method Development and Validation for Estimation of Teneligliptin in Tablet Dosage Form by RP-HPLC. Research J. Pharm. and Tech. 2020; 13(4): 1774-1778.
13. Sridharan D. Thenmozhi UA. Kumar LP. Chintalapati AD. Ramanaiah MV. Phanikishore Y. Development and Validation of UV Spectrophotometric Method of Darifenacin Hydrobromide in Bulk and Tablet Dosage Form. Asian J. Pharm. Ana. 2011; 1(3): 43-45.
14. Bishop P. Pitts ER. Budner D. Wittrick KAT. Kombucha:biochemical and microbiological impacts on the chemical and flavor profile. Food Chemistry Advances. 2022; 1: 100025.
15. Aung T. Eun JB. Impact of time and temperature on the physicochemical, microbiological and nutraceutical properties of laver kombucha (Porphyra dentata) during fermentation. LWT. 2022; 154: 112643.
16. Ebersole Y. Liu R. Schmidt M. Eckert PN. Brown. Determination of ethanol in kombucha products: Single-laboratory validation, first action 2016.12. Journal of AOAC International. 2017; 100 (3): 732-736.
17. Clayton B. Hymer. Residual solvent testing: A Review of Gas Chromatographic and Alternative techniques. Pharm Res. 2003; 23: 337.
18. Mary A. Jack HU. Pengu Z. Nina C. Simultaneous determination of formic acid and formaldehyde in pharmaceutical excipients using head space GC/MS. J Chrom: Biomedical Appl. 2006; 41: 783.
19. Babu JR. Suhasini J. Vidyadhara S. Residual Solvents in Bendamustine Hydrochloride By Headspace Chromatography. Asian J. Pharm. Ana. 2018; 8(1): 07-12.
20. Sudhaker S. Jain R. Effect of Using Propanol as Internal Standars on Quantitative Determination of Ethanol in Different Biological Matrices by Head Space-gas Chromatography-Flame Ionization Detector. Madridge Journal of Analytical Sciences and Instrumentation. 2016; 1(1): 1-3.
21. Sulthoniyah D. Primaharinastiti R. Annuryanti F. Method Validation of Flame Atomic Absorption Spectrophotometry (FAAS) for Determination of Iron (FE) in Multivitamin Mineral Capsule Dosage Form. Research J. Pharm. and Tech. 2018; 11(6): 2569-2574.
22. Indalkar YR. Aloorkar NH. Validation of Eugenol in Ethanol by UV Spectrophotometric Method. Asian J. Pharm. Ana. 2015; 5(4): 178-180.
23. Faridah DN. Solihat I. Yuliana ND. Validation of mineral (Fe, Zn and Cu) analysis method in carbohydrate, protein and fat-rich samples using microwave digestion method, Indones. J. Chem. 2020; 20(2): 348-359.
24. Kapupara P. Parekh D. Shah K. Method development and validation of Bronopol (Preservative) and its Stability Indicating Study by UV Spectrophotometry. Research J. Pharm. and Tech. 2016; 9(7): 916-918.
25. Sumithra M. Yuvanesh P. Mistry A. Analytical method development and validation of ambroxol hydrochloride by UV spectroscopy and forced degradation study and detection of stability. Research J. Pharm. and Tech. 2016; 9(7): 794-800.
26. Itigimatha N. Manjunatha DH. Yallur B. Liquid chromatographic method development and validation for the simultaneous determination of Phenylephrine hydrochloride, paracetamol, caffein and diphenhydramine hydrochloride. Research J. Pharm. And Tech. 2022; 15(9): 3993-3998.
27. Shrikrishna B. Nisharani R. Analytical method development and validation for simultaneous estimation of Montelukast and Ebastine by HPLC. Research J. Pharm. and Tech. 2015; 8(1): 01-05.