Author(s):
Anant Kumar Shrivastava, Shikhar Verma, Himani Awasthi
Email(s):
shikhar.verma@muit.in
DOI:
10.52711/0974-360X.2024.00590 
Address:
Anant Kumar Shrivastava1, Shikhar Verma2*, Himani Awasthi3
1Research Scholar, Maharishi School of Pharmaceutical Sciences, Maharishi University of Information Technology (MUIT), IIM Road, Indrapuri Colony, Diguria, Aziz Nagar, Lucknow, Uttar Pradesh 226013.
2Professor, Maharishi School of Pharmaceutical Sciences, Maharishi University of Information Technology (MUIT), IIM Road, Indrapuri Colony, Diguria, Aziz Nagar, Lucknow, Uttar Pradesh 226013.
3Professor, Hygia Institute of Pharmaceutical Education and Research, Ghaila Road, Balrampur, Lucknow, Uttar Pradesh 226020, India.
*Corresponding Author
Published In:
Volume - 17,
Issue - 8,
Year - 2024
ABSTRACT:
Prosopis juliflora (Sw.) commonly known as jangli babool or vilayati babool have several bioactive compounds of therapeutic needs. The presence of various bioactive compounds and markers specifically, quercetin and gallic acid in the methanolic extract of Prosopis juliflora (fruiting aerial part) denotes their valuable pharmacological activities. Therefore, high performance thin layer chromatography (HPTLC) analysis with simultaneous quantification of quercetin and gallic acid was done for their phytochemical profiling.The phytochemical profiling and simultaneous quantification of quercetin and gallic acid of methanolic extract of Prosopis juliflora through CAMAG HPTLC analysis was determined and results were obtained in the form of chromatogram scanned at the wavelength 254nm and 366nm. Phytochemical profiling of the plant was presented in the tables showing the number of peaks, peak area, area percentage and Rf values. 14 compounds were present in sample 1 and 9 compounds were present in sample 2 denoted by the number of peaks as represented in chromatograms of test samples. Peak area and height were used to quantify quercetin and gallic acid. Quercetin was found to be 1.364% in sample 1 and 0.27% in Sample 2 while gallic acid was 0.088% in sample 1 only. Importance of this research is to rationalize the therapeutic properties of Prosopis juliflora in the indian traditional medicinal system and phytochemical profiling and simultaneous quantification of quercetin and gallic acid reveals that Prosopis juliflora have several bioactive compounds and this might be accountable for their future application in the field of pharmacognostic and pharmacological drug discovery.
Cite this article:
Anant Kumar Shrivastava, Shikhar Verma, Himani Awasthi. HPTLC Phytochemical Profiling and Simultaneous Quantification of Quercetin and Gallic in Prosopis juliflora (Sw.). Research Journal of Pharmacy and Technology. 2024; 17(8):3801-6. doi: 10.52711/0974-360X.2024.00590
Cite(Electronic):
Anant Kumar Shrivastava, Shikhar Verma, Himani Awasthi. HPTLC Phytochemical Profiling and Simultaneous Quantification of Quercetin and Gallic in Prosopis juliflora (Sw.). Research Journal of Pharmacy and Technology. 2024; 17(8):3801-6. doi: 10.52711/0974-360X.2024.00590 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2024-17-8-38
REFERENCES:
1. Patil J. J KI. Kumar K. KG K. Prosopis juliflora. Res. J. Pharmacology and Pharmacodynamics. 2016; 8(4): 175-180. doi:10.5958/2321-5836.2016.00032.X
2. Mary Rose DL. Arockianathan PM. Lawrance A. Felicita AS. In-Vitro Study of Anti-diabetic, Anti-cancer and Anti-urolithiatic Activity of Different Extracts of Prosopis Juliflora Seeds. International Journal of Pharmaceutical Sciences and Research. 2021; 13(5): 2101-08. doi: 10.13040/IJPSR. 0975-8232.13(5).2101-08
3. Hassan SM. Taha AM. Eldahshan OA. Sayed AA. Salem AM. Modulatory effect of Prosopis juliflora leaves on hepatic fibrogenic and fibrolytic alterations induced in rats by thioacetamide. Biomedicine and Pharmacotherapy. 2019; 115: 108788. doi: 10.1016/j.biopha.2019.108788
4. Tajbaksh S. Barmak A. Vakhshiteh F. Gharibi M. Invitro Antibacterial Activity of the Prosopis Juliflora Seed Pods on Some Common Pathogens. J Clin Diagn Res. 2015; 9(8): 13-15. doi: 10.7860/JCDR/2015/13549.6370
5. Rad SJ. Kobarfard F. Martins N. Prosopis plant chemical composition and pharmacological attributes: Targeting clinical studies from preclinical evidence. Biomolecules. 2019; 9(12): 777. doi: 10.3390/biom9120777
6. Sagar K. Shivashankar PN. Amarappa S. Phytochemical Studies and Quantification of Total Phenols,Tannins and Flavanoids in Less Known Semi-arid Plants of Ballari, Karnataka. International Journal of Pharmaceutical Sciences and Research. 2021; 13(5): 2182-87. doi: 10.13040/IJPSR.0975-8232.13(5).2182-87
7. Ukande DM. Shaikh S. Murthy KS. A Review on pharmacological potentials of Prosopis juliflora. Journal of Drug Delivery and Therapeutics. 2019; 9(4-s): 755-760. doi: https://doi.org/10.22270/jddt.v9i4-s.3372
8. Mehari ZH. The invasion of Prosopis juliflora and Afar pastoral livelihoods in the Middle Awash area of Ethiopia. Ecol Process. 2015; 13(4): 0039-8. doi:https://doi.org/10.1186/s13717-015-0039-8
9. Khandelwal P. Sharma RA. Agarwal M. Isolation and identification of steroids from different parts of Prosopis juliflora. Int. J. Curr. Pharm. Res. 2017; 9(3): 54-57. doi: http://dx.doi.org/10.22159/ijcpr.2017v9i3.18896
10. Tadros MJ, Al-Assaf A, Othman YA, Makhamreh Z, Taifour H. Evaluating the Effect of Prosopis juliflora, an Alien Invasive Species, on Land Cover Change Using Remote Sensing Approach. Sustainability. 2020; 12(15): 5887. https://doi.org/10.3390/su12155887
11. Jain V. Jain T. Saraf S. Saraf S. HPTLC method for routine quality control of Ayurvedic formulation Drakshadi gutika. Asian J. Pharm. Ana. 2013; 3(4): 111-114. https://ajpaonline.com/AbstractView.aspx?PID=2013-3-4-1
12. Krishna K.V.V.S, Sankar KG, Vardhan MS, Krishna SR, Tamizhamani T. Simultaneous Estimation of Gallic acid and Rutin in Kaishore guggulu and Vatari guggulu by HPTLC. Res. J. Pharmacognosy and Phytochem. 2018; 10(3): 221-225. doi-10.5958/0975-4385.2018.00036.5
13. Prajapati LM. Patel A. Patel JR. Joshi AK. Kharodiya M. High-performance Thin Layer Chromatography method for simultaneous estimation of cafepime hydrochloride and sulbactum sodium. Asian J. Pharm. Ana. 2016; 6(4): 207-212. doi:10.5958/2231-5675.2016.00031.4
14. Jain V, Jain T, Saraf S. HPTLC method for routine quality control of ayurvedic formulation drakshadi gutka. Asian J. Pharm Ana. 2013; 3(4): 111-114. doi: 10.1016/j.jaim.2017.07.006
15. Goswami S. Singh RP. Gilhotra RM. HPTLC Fingerprinting of Quercetin and comparative assessment of Anthelmintic potency against Eudrilus eugeniae of Schleichera oleosa (Lour) Oken and Tagetes eretca Linn. Research J. Pharm. and Tech. 2020; 13(12): 5699-5704. doi: 10.5958/0974-360X.2020.00992.0
16. Ramasamy Aiyalu Rajasekaran. Detection and Quantification of anti-oxidant markers like rutin, catechin, quercetin, gallic acid, ellagic acid, ferulic acid, vitexin and mangiferin in herbal raw materials available in the market belong to rutaceae family by HPTLC technique. Asian Journal of Pharmaceutical Analysis. 2021; 11(2): 151-5. doi: 10.52711/2231-5675.2021.00026
17. Nikam PH. Kareparamban J. Jadhav A. Kadam V. Future Trends in Standardization of Herbal Drugs. Journal of Applied Pharmaceutical Science. 2012;2(6). doi:10.7324/JAPS.2012.2631
18. Singh V. Rizvi A. Sara UV. Standardization and Phytochemical Screening of Carica papaya Seeds. Res. J. Pharm. and Tech. 2021; 14(9): 4540-6. doi:10.52711/0974-360X.2021.00790
19. Sasikala M. Mohan S. Nandha K. Kumar Sathish. Varshini N. HPTLC and Antioxidant activity of Ethanolic Extract of Ipomoea obscura (L.) Ker Gawl Stem. Research J. Pharm. and Tech. 2020; 13(10): 4661-4667. doi:10.5958/0974-360X.2020.00820.3
20. Rode VP, Tajne MR. A Validated Stability-Indicating High-Performance Thin-Layer Chromatographic Method for the Analysis of Pitvastatin in Bulk Drug and Tablet Formulation. Asian J. Pharm. Ana. 2018; 8(1): 49-52. doi-10.5958/2231-5675.2018.0009.1