Author(s):
Ch. Bhavani, V. Padmabhushana Chary, P.Mahitha, R. Mahesh, R. Chenchulaxmi, M. Chinnaeswaraiah
Email(s):
padmabhushanchary@gmail.com
DOI:
10.52711/0974-360X.2025.00678 
Address:
Ch. Bhavani1, V. Padmabhushana Chary1*, P.Mahitha1, R. Mahesh1, R. Chenchulaxmi1, M. Chinnaeswaraiah2
1Department of Pharmaceutical Analysis, Anurag Pharmacy College, Kodad-508206, Suryapeta (Dist), Telangana, India.
2Department of Pharmacognosy, Anurag Pharmacy College, Kodad-508206, Suryapeta (Dist), Telangana, India.
*Corresponding Author
Published In:
Volume - 18,
Issue - 10,
Year - 2025
ABSTRACT:
For the development and validation of the Paroxetine Hydrochloride method, a simple, reliable, cost-effective, and repeatable UV Spectrophotometric method was used. With a correlation value (R2 = 0.999), paroxetine hydrochloride exhibits maximum absorbance (?max) in methanol at 294 nm and follows Beer's law at concentrations between 10 - 60 µg/ml. By using the standard addition approach, the proposed method's validity was evaluated. The additional standard's mean percentage recovery was found to be between 98% and 102%. The determined values for the limits of detection and quantification were 0.206µg/ml and 0.625µg/ml, respectively. The International Conference for Harmonisation (ICH) Q2 (R1) guidelines have been followed in the validation of the method. The suggested approach is rapid, simple to use, precise, sensitive, and cost-effective, making it suitable for the regular quantification of paroxetine in pharmaceutical formulations and bulk form.
Cite this article:
Ch. Bhavani, V. Padmabhushana Chary, P.Mahitha, R. Mahesh, R. Chenchulaxmi, M. Chinnaeswaraiah. Development of a UV Spectrophotometric Method and Validation of Paroxetine Hydrochloride. Research Journal of Pharmacy and Technology. 2025;18(10):4716-0. doi: 10.52711/0974-360X.2025.00678
Cite(Electronic):
Ch. Bhavani, V. Padmabhushana Chary, P.Mahitha, R. Mahesh, R. Chenchulaxmi, M. Chinnaeswaraiah. Development of a UV Spectrophotometric Method and Validation of Paroxetine Hydrochloride. Research Journal of Pharmacy and Technology. 2025;18(10):4716-0. doi: 10.52711/0974-360X.2025.00678 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2025-18-10-17
REFERENCES:
1. Sonali S. Gadge, Madhuri D. Game, Vikrant L. Salode. Simultaneous Spectrophotometric Estimation of Paroxetine Hydrochlorides and Clonazepam in Bulk and Tablet Dosage Form. Research Journal of Pharmacy and Technology. 2021; 14(5): 2497-1. doi: 10.52711/0974-360X.2021.00440.
2. Kumaran SC Sreedhar, T SrinivasaRao, Harsha K Tripathy. UV-Vis Spectroscopy method to determination and validation of paroxetine hydrochloride in pure and tablet dosage form; International Journal for Research Trends and Innovation. 2022; 7(6). 1358 -1363.
3. Siva Shanker Reddy. L, Rajkumar T. and Jenny Sushmitha Evangiline D. Method development and validation of visible spectroscopic method for the estimation of Paroxetine Hydrochloride in pure and tablet dosage form. Chem Sci Trans., 2017; 6(4): 679- 684.
4. Hemangi Parekh and Rajashree Mashru. Development and validation of analytical method forsimultaneous estimation of paroxetine HCL and etizolam; The Pharma Innovation Journal 2020; 9(9): 145-153.
5. Sheetal, Sonia. K, K. S. Lakshmi. Validation of Telmisartan by UV Spectrophotometry Method. Research J. Pharm. and Tech. 2019; 12(5): 2413-2415. doi: 10.5958/0974-360X.2019.00404.9.
6. Muchlisyam Bachri, Sukma Safarul Rizky. UV Spectrophotometry with Chemometric methods for Concurrent Assays of Antihypertensive Components in Tablets. Research Journal of Pharmacy and Technology 2023; 16(9): 4314-8. doi: 10.52711/0974-360X.2023.00706
7. Roshan Telrandhe. Development and Validation of UV Spectrophotometry and RP-HPLC Method for simultaneous determination of Rosuvastin and Clopidogrel in Tablet Dosage Form. Asian J. Pharm. Ana. 2018; 8(1): 25-32. doi: 10.5958/2231-5675.2018.00005.4
8. Audumbar Digambar Mali. Simultaneous Determination of Carvedilol and Hydrochlorothiazide in Pharmaceutical Dosage Form by Second Order Derivative UV Spectrophotometry. Asian J. Pharm. Ana. 2015; 5(3): 133-138. doi: 10.5958/2231-5675.2015.00021.6
9. G. Krishnamoorthy, C. Diana Priyadarshini, R. Senthamarai. Spectrophotometric method of Choline Bitartrate in bulk and its tablet formulation. Asian J. Pharm. Ana. 2012; 2(4): 114-115.
10. D. Sridharan, Umarani A. Thenmozhi, L. Pavan Kumar, Aswani Dutt Chintalapati, M. Venkata Ramanaiah, YelikaPhanikishore. Development and Validation of UV Spectrophotometric Method of Darifenacin Hydrobromide in Bulk and Tablet Dosage Form. Asian J. Pharm. Ana. 2011; 1(3): 43-45.
11. Mrudula Kulkarni, Pratibha Dange, Sanjay Walode. Development and Validation of Difference Spectrophotometric Method for Zotepine in Bulk and Tablet Dosage Form. Asian J. Pharm. Ana. 2013; 3(3): 105-107.
12. B. Siddartha, I. SudheerBabu, A. Krupalini, Prathyusha V. Development and Validation of UV– Spectrophotometric Method of Tolterodine in bulk andPharmaceutical Dosage Form. Asian J. Pharm. Ana. 2013; 3(3): 102-104.
13. Agrawal O.D, TelangN.B . Development and Validation of UV Spectrophotometric Method for Estimation of Benfotiamine in Bulk and Dosage Form. Asian J. Pharm. Ana. 2016; 6(3): 133-137.
14. Pandya Dimple Bhupendra, Shinkar Dattatraya Manohar, Saudagar Ravindra Bhanudas, BacchavJyoti Kailas. UV Spectrophotometric Method for Estimation of Eprosartan Mesylate in Bulk and in Pharmaceutical Formulation. Asian J. Pharm. Ana. 2016; 6(2): 119-121.
15. Kailash Nath Kaushik, Saurabh Kumar Banerjee. UV Spectrophotometric Method Development for the Determination of Domperidone in Tablet Formulation. Asian J. Research Chem. 2009; 2(4): 432-433.
16. Santosh Shelke, Santosh Dongre, AmitRathi, Dinesh Dhamecha, Saifee Maria, Mohd Hassan G Dehghan. Development and Validation of UV Spectrophotometric Method of Cefuroxime Axetil in Bulk and Pharmaceutical Formulation. Asian J. Research Chem. 2009; 2(2): 222-224.
17. N.Harikrishnan, Deepthi R, Manjusha V, P SatyaVani, MV AshaJyothi, C Roosewelt. Development and Validation of UV Spectrophotometric Methods of Loratadine in Bulk and Pharmaceutical Formulation. Asian J. Research Chem. 2010; 3(2): 302-304.
18. Pallavi Salve, Deepali Gharge, Rupali Kirtawade, Pandurang Dhabale, KishorBurade. Simple Validated Spectroscopic Method for Estimation of Amlodipine Besylate from Tablet Formulation. Asian J. Research Chem. 2009; 2(4): 553-555.
19. Prabhakar Panzade, Prashant Puranik, Vipul Mogal, Mayur Patni. Development and Validation of UV Spectrophotometric Method of Granisetron Hydrochloride in Bulk and Pharmaceutical Formulation. Asian J. Research Chem. 2010; 3(3): 634-636.
20. Zahid Zaheer, Obaid Shaikh, Sucheta Thorat, Rana Z. Ahmed. Development and Validation of UV Spectrophotometric Method of Fluoxetine Hydrochloride in Bulk and Pharmaceutical Formulation. Asian J. Research Chem. 2010; 3(3): 545-548.
21. Rele R.V., Patil S.P. Ultra-Violet Spectrophotometric Method for Estimation of Azelnidipine from Bulk Drug and Pharmaceutical Formulation. Asian J. Research Chem. 2010; 3(4): 1077-1079.
22. Singh Kumar Rakesh, Patel Singh Pankaj, Singh Amar, Roy R.K. A Simple UV Spectrophotometric Method Development and Validation for Estimation of Ciprofloxacin Hydrochloride in Bulk and Tablet Dosage Form. Asian J. Research Chem. 2012; 5(3): 336-339.
23. Raveendra Babu G., Praveen Kumar J., Sri Lakshmi Surekha P., Kala Praveen T., SambhasivaRao P. Development and Validation of UV Spectrophotometric Method of Amlodipine Besylste in Bulk and Pharmaceutical Formulation. Asian J. Research Chem. 2014; 7(6): 596-599.
24. Sheeja V.K., Swapna A.S., Sosamma Cicy Eapen, Kumar P. Method Development and Validation for the Simultaneous Estimation of Clonazepam and Paroxetine in Combined Dosage form using Colorimetry. Asian J. Research Chem. 2014; 7(1): 48-51.
25. Sushil D. Patil, Sayali K. Chaure, Maswood Ahmed Hafizur Rahman, Prajkta U. Varpe, Sanjay Kshirsagar.. Development and Validation of Simple UV- Spectrophotometric Method for the Determination of Empagliflozin. Asian J. Pharm. Ana. 2017; 7(1): 18-22. doi: 10.5958/2231-5675.2017.00004.7.
26. Sushil D. Patil, Sayali K. Chaure, Sanjay Kshirsagar. Development and validation of UV spectrophotometric method for Simultaneous estimation of Empagliflozin and Metformin hydrochloride in bulk drugs. Asian J. Pharm. Ana. 2017; 7(2): 117-123. doi: 10.5958/2231-5675.2017.00019.9
27. Sireesha. D, M. Laksmi Monika, Vasudha Bakshi. Development and Validation of UV Spectrophotometric Method for the simultaneous estimation of Rosuvastatin and Ezetimibe in Pharmaceutical Dosage Form. Asian J. Pharm. Ana. 2017; 7(3): 135-140. doi: 10.5958/2231-5675.2017.00021.7
28. Omkar A. Patil, Indrajeet S. Patil, Ganesh B. Vambhurkar, Dheeraj S. Randive, Mangesh A. Bhutkar, Srinivas K. Mohite. UV Spectroscopic Degradation Study of Pioglitazone Hydrochloride. Asian J. Pharm. Ana. 2018; 8(3): 125-128. doi: 10.5958/2231-5675.2018.00023.6
29. Sri Lakshmi D, Jane T Jacob, Srinivasa Sastry D, Satyanarayana D. Simultaneous Estimation of Metformin Glimeperide and Voglibose by RP-UPLC. Asian J. Pharm. Ana. 2017; 7(1): 23-30. doi: 10.5958/2231-5675.2017.00005.9.
30. Komal P. Shinde, Akash D. Rajmane. A Review UV Method Development and Validation. Asian Journal of Pharmaceutical Analysis. 2023; 13(2): 122-0. doi: 10.52711/2231-5675.2023.00021.
31. Zainab A. Bagalkote, Ganesh Gajeli. UV Spectrophotometric Method Development and Validation of Carbimazole in Bulk and Tablet Dosage form. Asian Journal of Pharmaceutical Research. 2021; 11(3): 163-6. doi: 10.52711/2231-5691.2021.00030.
32. Priya, ArchanaGahtori. UV Spectrophotometric Method Development and Validation for Amlodipine Besylate in Bulk and Tablet Dosage Form. Asian J. Pharm. Ana. 2022; 12(2): 94-8. doi: 10.52711/2231-5675.2022.00017
33. K. Vijaya Sri, S. Sravani, M. Shiva Kumar. Development and Validation of UV Spectrophotometric Method for Estimation of Lurasidone in Bulk and Pharmaceutical Formulations. Asian J. Pharm. Res. 2015; 5(2): 102-107. doi: 10.5958/2231-5691.2015.00015.5.
34. Rajashri R. Kulkarni, Dipti G. Phadtare, Ravindra B. Saudagar. UV Spectrophotometric Method Development and Validation of Fluticasone Propionate. Asian J. Res. Pharm. Sci. 2016; 6(2): 135-138. doi: 10.5958/2231-5659.2016.00019.9.
35. Anjaneyulu.Vinukonda, K. B. Chandra Sekhar, ShaikMuneer, B. Siva SaiKiran, Akkimi Padma, Pallavi A. Method Development and Validation for the Quantification of Cyamemazine tartrate (CYMT) in bulk and its marketed formulation by using UV Spectroscopy. Asian J. Pharm. Tech. 2019; 9(1): 08-10. doi: 10.5958/2231-5713.2019.00002.3.
36. Raveendra Babu Konduri, T Bhavani, P Srividya, M Chinna Eswaraiah Validated UV Spectroscopic Method for Determination of Domperidone in Bulk and Pharmaceutical Formulation UV Spectroscopic Method. Research J. Pharm. and Tech. Technology. 2025: 18(1): 317-320.