Author(s):
Susilowati, Ardy Prian Nirwana, Dwi Koko Pratoko, Nastiti Utami, Selsa Rizky Widya Ariyanto
Email(s):
susilowati@stikesnas.ac.id
DOI:
10.52711/0974-360X.2025.00749 
Address:
Susilowati, Ardy Prian Nirwana, Dwi Koko Pratoko, Nastiti Utami, Selsa Rizky Widya Ariyanto
Sekolah Tinggi Ilmu Kesehatan Nasional, Sukoharjo, Central Java, 57552, Indonesia
Faculty of Pharmacy, Universitas Jember, Jember, Indonesia, 68121
*Corresponding Author
Published In:
Volume - 18,
Issue - 11,
Year - 2025
ABSTRACT:
Palmarosa essential oil (PEO) was extracted from the leaves, stems, and herbs of Cymbopogon martinii using steam distillation. GC-MS analysis was used for identifying and quantifying the PEO components, cytotoxicity activity on the T-47D cell line was tested by MTT assay, and apoptosis induction was monitored by double staining (ethidium bromide-orange acridine). Docking simulations of PEO compounds were tested using the PyRx 0.8 program against estrogen receptor alpha (PDB ID: 1XP1) and progesterone receptor (PDB ID: 4OAR) proteins. PEO from each part of C. martinii showed different compositions, nevertheless, geraniol and geranyl acetate were recognized as major components. Furthermore, cytotoxicity activity (IC50) results showed that the leaves PEO has the strongest activity than stems and herbs. This PEO was able to induce apoptosis of the T-47D cell line marked by orange fluorescence in cell morphology. Our molecular docking study demonstrated that 4,11-Selinadiene and ß-Caryophyllene have a higher binding affinity than other PEO compounds. In addition, 4,11-Selinadiene was only found in leaves and ß-Caryophyllene was higher in leaves than stems and herbs PEO. It could be concluded that these two compounds were responsible as inhibitors against the T-47D breast cancer cell line.
Cite this article:
Susilowati, Ardy Prian Nirwana, Dwi Koko Pratoko, Nastiti Utami, Selsa Rizky Widya Ariyanto. Palmarosa Essential Oil Study: Composition, Cytotoxicity and Molecular Docking on T-47D. Research Journal Pharmacy and Technology. 2025;18(11):5191-0. doi: 10.52711/0974-360X.2025.00749
Cite(Electronic):
Susilowati, Ardy Prian Nirwana, Dwi Koko Pratoko, Nastiti Utami, Selsa Rizky Widya Ariyanto. Palmarosa Essential Oil Study: Composition, Cytotoxicity and Molecular Docking on T-47D. Research Journal Pharmacy and Technology. 2025;18(11):5191-0. doi: 10.52711/0974-360X.2025.00749 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2025-18-11-11
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