Author(s):
Hardik Jeshti, Kajal Vable, Krishna Kalsara, Mitali Dalwadi, Aakash Vasava, Umesh Upadhayay
Email(s):
vablekajal@gmail.com
DOI:
10.52711/0974-360X.2025.00750 
Address:
Hardik Jeshti, Kajal Vable*, Krishna Kalsara, Mitali Dalwadi, Aakash Vasava, Umesh Upadhayay
Department of Quality Assurance, Sigma Institute of Pharmacy, Sigma University, Vadodara - 390019, Gujarat, India.
*Corresponding Author
Published In:
Volume - 18,
Issue - 11,
Year - 2025
ABSTRACT:
Simultaneous measurement of GLIM and LINA in a synthetic mixture had been accomplished by validation and development of a UV spectrophotometric technique. Technique utilized overlain zero-order spectra with absorbance measurements at 242nm for Glimepiride and 225nm for Linagliptin in methanol. Linearity had been observed in range of 1 to 8µg/mL for Glimepiride and 5 to 40µg/mL for Linagliptin. Method showed excellent precision with %RSD values below 1% for both repeatability and interday precision. Recovery studies confirmed 100% recovery at selected concentrations, indicating accuracy. LOQ and LOD values had been discovered to be 0.28 to 0.30µg/ml and 0.38 to 0.42µg/ml, correspondingly. Assay outcomes of 97.69% for Glimepiride and 101.84% for Linagliptin confirmed the method’s applicability for analysis in synthetic mixtures.
Cite this article:
Hardik Jeshti, Kajal Vable, Krishna Kalsara, Mitali Dalwadi, Aakash Vasava, Umesh Upadhayay. Development and Validation of UV Spectroscopy Simultaneous Estimation of Glimepiride and Linagliptin in A Synthetic Mixture. Research Journal Pharmacy and Technology. 2025;18(11):5201-6. doi: 10.52711/0974-360X.2025.00750
Cite(Electronic):
Hardik Jeshti, Kajal Vable, Krishna Kalsara, Mitali Dalwadi, Aakash Vasava, Umesh Upadhayay. Development and Validation of UV Spectroscopy Simultaneous Estimation of Glimepiride and Linagliptin in A Synthetic Mixture. Research Journal Pharmacy and Technology. 2025;18(11):5201-6. doi: 10.52711/0974-360X.2025.00750 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2025-18-11-12
REFERENCE:
1. Patel KS, Damahe N, Narkhede SB, et al Development and Validation of UV Spectroscopic Method for Simultaneous Estimation of Glimepiride and Linagliptin in Their Synthetic Mixture. International Journal of Pharmaceutical Research and Applications. 2024; 9(3): 2536-2545. 10.35629/4494-090325362545
2. Atole D, Rajput H et al Ultraviolet Spectroscopy and Its Pharmaceutical Applications - A Brief Review. Asian Journal of Pharmaceutical and Clinical Research. 2018; 11(2): 59-66. http://dx.doi.org/10.22159/ajpcr.2018.v11i2.21361
3. Sacide A, Dilara T, et al Analysis of Glimepiride by Using Derivative UV Spectrophotometric Method. Journal of Pharmaceutical and Biomedical Analysis. 2001; 24(3): 507-515. https://doi.org/10.1016/S0731-7085(00)00445-3
4. Sakala B, Gopisetty S, Krishna D, Kota S et al. UV Spectrophotometric Method for Determination of Glimepiride in Pharmaceutical Dosage Forms. International Journal of Pharmaceutical Sciences Review and Research. 2013; 21(2): 131-133.
5. Rudy B, Mangali B, Herida S, et al. Development and Validation of a UV-Derivative Spectrophotometric Method for Determination of Glimepiride in Tablets. Journal of the Brazilian Chemical Society. 2011; 22(2): 292-299. 10.1590/S0103-50532011000200015
6. Santosh K, Sunayana M, Ramaling K et al. Novel First Order Derivative UV Spectrophotometric Method for Determining Glimepiride in Solid Dosage Forms. International Journal of Drug Delivery Technology. 2018; 8(3): 121-126. 10.25258/ijddt.8.3.2
7. Abdul A, Souad Z, et al Direct Spectrophotometric Determination of Glimepiride in Pure Form and Pharmaceutical Formulations Using Bromocresol Purple. Journal of Advances in Chemistry. 2018; 15(2): 6186-6198. 10.24297/jac.v15i2.7454
8. Abdul A, Hasana H, Zeino et al. Spectrophotometric Determination and Validation of Glimepiride in Pure and Tablet Dosage Forms Through Ion-Pair Complex Formation Using Bromocresol Green. International Journal of Pharmacy and Pharmaceutical Sciences. 2016; 8(6): 216-221. 10.5958/0974-360X.2018.00561.9
9. Nermine S, Emad H, Madonna A, Maha H et al. Spectrophotometric Methods for Determination of Glimepiride and Pioglitazone Hydrochloride Mixture and Application in Their Pharmaceutical Formulation. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 2022; 270: 120745. https://doi.org/10.1016/j.saa.2021.120745
10. Asmaa A, Ehab E, Hanan M, Mahmoud A et al. Simultaneous Spectrophotometric Determination of Glimepiride and Pioglitazone in Binary mixture and combined dosage form using chemometrics-assisted techniques. Spectrochimica acta part A: Molecular and biomolecular Spectroscopy. 2017; 182: 175-182. https://doi.org/10.1016/j.saa.2017.03.028
11. Thomas A, Sandip B, Lata K, Sumitra J et al. Simultaneous Spectrophotometric Estimation of Pioglitazone, Metformin HCl, and Glimepiride in Bulk and Formulation. Asian Journal of Chemistry. 2007; 19(5): 3821-3830.
12. Dalal A, Tegeli V, Waghmode R et al. Development and Validation of a Simple and Rapid UV Spectrophotometric Method for Linagliptin in Bulk and Marketed Dosage Form. Der Pharma Chemica. 2022; 14(2): 23-26.
13. Rane S, Chaudhari R, Patil V, Barde L et al. Development and Validation of UV Spectrophotometric Method for Simultaneous Estimation of Empagliflozin and Linagliptin in Bulk Drugs and Pharmaceutical Dosage Form. International Journal of Doctrines of Integrative Medicine, Pharmacy and Science. 2021; 01(01): 44-53.
14. Sen A, Pandey H, Maheshwari R, Zanwar A, Velmurugan R, Sen D, et al. Novel UV Spectroscopic Methods for Simultaneous Assessment of Empagliflozin, Linagliptin, and Metformin in Ternary Mixture. Indian Journal of Pharmaceutical Education and Research. 2022; 56(4): 669-681. 10.5530/ijper.56.4s.213
15. Mishra M, Verma G et al Analytical Method Development and Validation for Determination of Linagliptin in Bulk and Dosage Form by UV Spectroscopy. Journal of Emerging Technologies and Innovative Research. 2018; 5(7): 908-912.