Author(s):
Manvi Sharma, Aakansha, Madhulika Esther Prasad, Ajam Shaikh, Chinmoyee Maharana, Amit Gupta
Email(s):
amit.gupta@jammuuniversity.ac.in
DOI:
10.52711/0974-360X.2025.00756
Address:
Manvi Sharma1, Aakansha1, Madhulika Esther Prasad2, Ajam Shaikh2, Chinmoyee Maharana1, Amit Gupta1*
1Department of Zoology, University of Jammu, Jammu and Kashmir, India.
2Department of Biochemistry and Biotechnology, School of Life Sciences, Sardar Bhagwan Singh University, Dehradun, Uttarakhand, India.
3Department of Science, Faculty of Humanities Liberal Arts and Social Sciences, Alliance University, Bengaluru, Karnataka, India.
*Corresponding Author
Published In:
Volume - 18,
Issue - 11,
Year - 2025
ABSTRACT:
Plants are rich in bioactive compounds and play an important role in the development of novel medications. In this study, lemon samples had been collected from different regions in the Jammu area, and their antioxidant and antibacterial qualities, and additionally their phenolic and flavonoid content, were explored. The purpose of this study was to determine the phenolic, flavonoid, antioxidant and antimicrobial content of a lemon peel extract. In order to achieve this objective, the Folin-Ciocalteu and aluminium chloride approaches was employed for calculating the overall amount of phenolic and flavonoid compounds of the lemon peel. The DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging technique was used to measure the antioxidant activity. Furthermore, antibacterial activity of lemon peel was assessed using the disc diffusion technique. From these studies, the results showed that the content of phenols and flavonoid ranged from 218.12 to 678.22 mg GAE/g and 8.06 to 171.37 mg quercetin/g, respectively. Its antioxidant activity of lemon peel was found to be greater, ranging from 24.49 to 65.71 mg AAE/g sample. Additionally, they demonstrated equal efficacy to the reference antibiotic i.e. Gentamicin and lemon peel of variable concentrations which showed maximum inhibition zones for Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli.
Cite this article:
Manvi Sharma, Aakansha, Madhulika Esther Prasad, Ajam Shaikh, Chinmoyee Maharana, Amit Gupta. Evaluation of the Phenolic, Flavonoid, Antioxidant and Active ingredients in Lemon peel extract. Research Journal Pharmacy and Technology. 2025;18(11):5245-0. doi: 10.52711/0974-360X.2025.00756
Cite(Electronic):
Manvi Sharma, Aakansha, Madhulika Esther Prasad, Ajam Shaikh, Chinmoyee Maharana, Amit Gupta. Evaluation of the Phenolic, Flavonoid, Antioxidant and Active ingredients in Lemon peel extract. Research Journal Pharmacy and Technology. 2025;18(11):5245-0. doi: 10.52711/0974-360X.2025.00756 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2025-18-11-18
REFERENCES:
1. El Aboubi M, Bikri S, Hsaini A, Berrid N, Aouane M. The protective effect of Lemon Peel Essential Oil on Pancreas and Brain Oxidative Stress and Inflammation induced by Streptozotocin-Nicotinamide in rats: Behavioral and Biochemical Evidences. Research Journal of Pharmacy and Technology. 2023; 16(10): 4897-6.
2. Shah RR, Gayakar PP, Nitalikar MM. Lemon Peel as a Potential Source of Pectin and it's use as Application of Pectin as Binder in the Formulation of Tablets. Research Journal of Pharmacy and Technology. 2015; 8(12): 1669-1672.
3. Patil PM, Nayakal OS, Bhutkar M, Bhinge S, Randive D. Extraction of Pectin from Lemon Peel and its use as a Natural Binder in Aspirin Tablet. Asian Journal of Pharmacy and Technology. 2018; 8(3): 115-118.
4. Abdelwahab AS, Abouelyazeed A. Bioactive Compounds in Some Citrus Peels as Affected by Drying Processes and Quality Evaluation of Cakes Supplemented with Citrus Peels Powder. Journal of the Advances in Agricultural Researches. 2018; 23(1): 44-67.
5. Al-Qudah TS, Zahra U, Rehman R, Majeed MI, Sadique S, Nisar S, Tahtamouni RW. Lemon as a source of functional and medicinal ingredient: A review. International Journal of Chemical and Biomedical Sciences. 2018; 14: 55–61.
6. Gonzalez-Molina E, Domínguez-Perles R, Moreno DA, Garcia-Viguera C. Natural bioactive compounds of Citrus limon for food and health. Journal of Pharmaceutical and Biomedical Analysis. 2010; 51(2): 327–345.
7. Mohanapriya M, Ramaswamy L, Rajendran R. Health and medicinal properties of lemon (Citrus limonum) International Journal of Ayurvedic and Herbal Medicine 2013; 3(1):1095–1100.
8. Klimek-Szczykutowicz, Szopa, Ekiert. Citrus limon (Lemon) Phenomenon-A Review of the Chemistry, Pharmacological Properties, Applications in the Modern Pharmaceutical, Food, and Cosmetics Industries, and Biotechnological Studies. Plants. 2020; 9(1): 119.
9. Singh B, Singh JP, Kaur A, Singh N. Phenolic composition, antioxidant potential and health benefits of citrus peel. Food Research International. 2020; 132: 109114.
10. Dubey R, Rajhans S, Mankad AU. Preliminary Phytochemical Screening, Quantitative Estimation of Total Phenolic and Flavonoid Content of Jatropha gossypiifolia (L.). Research Journal of Pharmacognosy and Phytochemistry. 2020; 12(2): 83-86.
11. Erlia AS, Sanjayadi BL, Monika AC, Agustina DRN. Quick-identification of total phenolic and flavonoid content of Mangifera foetida, Lagerstroemia speciosa and Impatiens balsamina gathered from Riau, Sumatera. Research Journal of Pharmacy and Technology. 2022; 15(1): 311-4.
12. Himalian R, Singh MP. A Comparative account on Antioxidant activities, Total phenolic and Flavonoid contents of Punica granatum, Carica papaya, Foeniculum vulgare, Trigonella foenum-graecum, and Urtica dioica: An in vitro Evaluation. Research Journal of Pharmacy and Technology. 2022; 15(3): 1175-3.
13. Chopade AR, Sayyad FJ. Evaluation of membrane stabilizing and inhibition of protein denaturation activity of Phyllanthus fraternus Webster. Research Journal of Pharmacy and Technology. 2013; 6(3): 251-254.
14. Ezhumalai M, Hemalatha G, Poornima JP, Pugalendi KV. Inhibition of Lactobacillus growth by amino acids and phytochemicals in the fermentation of curd by disc diffusion method. Asian Journal of Pharmacy and Research. 2013; 3(4): 189-193.