Author(s):
Amitkumar J. Vyas, Anjali V. Ramani, Harshal M. Vadile, Ajay I. Patel, Ashvin V. Dudhrejiya, Sunny R. Shah, Urvi J. Chotaliya, Shweta Prajapati, Devang B. Sheth
Email(s):
amitvyas77@gmail.com
DOI:
10.52711/0974-360X.2025.00762 
Address:
Amitkumar J. Vyas1, Anjali V. Ramani1*, Harshal M. Vadile1, Ajay I. Patel1, Ashvin V. Dudhrejiya1, Sunny R. Shah2, Urvi J. Chotaliya1,Shweta Prajapati1, Devang B. Sheth3
1B. K. Mody Government Pharmacy College, Rajkot - 360003, Gujarat, India.
2Government Pharmacy College, Gandhinagar, Gujarat, India.
3L. M. College of Pharmacy, Ahmedabad, Gujarat, India.
*Corresponding Author
Published In:
Volume - 18,
Issue - 11,
Year - 2025
ABSTRACT:
This study developed an RP-HPLC method to simultaneously determine Silodosin and Tadalafil. Using a Thermo BDS C18 column with a mobile phase of dipotassium hydrogen phosphate buffer and acetonitrile (60:40), the method achieved retention times of 2.905 minutes for Silodosin and 5.082 minutes for Tadalafil. The method exhibited excellent linearity with a correlation coefficient of 0.999 for both drugs, with Silodosin ranging from 1.6-200µg/mL and Tadalafil from 1-125µg/mL. The accuracy was between 99.24% and 100.97% for Silodosin and 99.92% and 100.13% for Tadalafil. The limits of detection were 0.40µg/mL for Silodosin and 0.20µg/mL for Tadalafil, while the limits of quantification were 1.21µg/mL and 0.63µg/mL, respectively. This method is precise and accurate, making it suitable for routine analysis of these drugs.
Cite this article:
Amitkumar J. Vyas, Anjali V. Ramani, Harshal M. Vadile, Ajay I. Patel, Ashvin V. Dudhrejiya, Sunny R. Shah, Urvi J. Chotaliya, Shweta Prajapati, Devang B. Sheth. Stability Indicating RP-HPLC Method Development and Validation for Simultaneous Estimation of Silodosin and Tadalafil in Synthetic Mixture. Research Journal Pharmacy and Technology. 2025;18(11):5288-2. doi: 10.52711/0974-360X.2025.00762
Cite(Electronic):
Amitkumar J. Vyas, Anjali V. Ramani, Harshal M. Vadile, Ajay I. Patel, Ashvin V. Dudhrejiya, Sunny R. Shah, Urvi J. Chotaliya, Shweta Prajapati, Devang B. Sheth. Stability Indicating RP-HPLC Method Development and Validation for Simultaneous Estimation of Silodosin and Tadalafil in Synthetic Mixture. Research Journal Pharmacy and Technology. 2025;18(11):5288-2. doi: 10.52711/0974-360X.2025.00762 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2025-18-11-24
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